US4450233A
Expired / Lapsed
US
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Immobilization of microorganisms in a polymer gel
Assignee: Asahi Kasei Corp
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Filing: 1981-12-15
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Granted: 1984-05-22
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Expires: 2001-12-15
Abstract
Abstract Microorganisms are immobilized by adding microorganism cells to an aqueous solution of a mixture of a polymerizable starch and a polymerizable monomer, and, thereafter, polymerizing the polymerizable starch and polymerizable monomer, to prepare a polymer gel with microorganism cells enclosed therein. The polymerizable starch is prepared by introducing an acrylamidomethyl group into starch. The polymer gel has high mechanical strength and can be used repeatedly over long periods of the time while maintaining at high levels the reactivity of the microorganism enclosed therein.
Inventors
- 👤 Akio Mimura
- 👤 Katsumi Yuasa
- 👤 Mitsuru Shibukawa
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 18:28
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C12N11/10; C12N11/04
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
83 words
A process for producing an immobilized microorganism, comprising adding microorganism cells to an aqueous solution of a mixture of a polymerizable starch and a polymerizable vinyl monomer, said polymerizable starch being prepared by introducing an acrylamidomethyl group into starch and said polymerizable starch being present in an amount of about 5 to 80 weight percent based on the total weight of the polymerizable starch and the polymerizable monomer, and thereafter, polymerizing the mixture, to prepare a polymer gel with microorganism cells enclosed therein.
Claim 2
Dependent Claim
83 words
A process for producing an immobilized microorganism, comprising adding microorganism cells to an aqueous solution of a mixture of a polymerizable starch and a polymerizable vinyl monomer, said polymerizable starch being prepared by introducing an acrylamidomethyl group into starch and said polymerizable starch being present in an amount of about 5 to 80 weight percent based on the total weight of the polymerizable starch and the polymerizable monomer, and thereafter, polymerizing the mixture, to prepare a polymer gel with microorganism cells enclosed therein.
Claim 3
Dependent Claim
29 words
A process as in claim 1, wherein the polymerizable monomer is at least one vinyl monomer selected from the group consisting of acrylamide, acrylic acid, hydroxymethylacrylic acid, and N,N'-methylenebisacrylamide.
Claim 4
Dependent Claim
86 words
A polymer gel containing immobilized microorganisms made by a process comprising adding microorganism cells to an aqueous solution of a mixture of a polymerizable starch and a polymerizable vinyl monomer, said polymerizable starch being prepared by introducing an acrylamidomethyl group into starch and said polymerizable starch being present in an amount of about 5 to 80 weight percent based on the total weight of the polymerizable starch and the polymerizable monomer, and, thereafter, polymerizing the mixture to prepare a polymer gel with microorganism cells enclosed therein.
Claim 5
Dependent Claim
86 words
A polymer gel containing immobilized microorganisms made by a process comprising adding microorganism cells to an aqueous solution of a mixture of a polymerizable starch and a polymerizable vinyl monomer, said polymerizable starch being prepared by introducing an acrylamidomethyl group into starch and said polymerizable starch being present in an amount of about 5 to 80 weight percent based on the total weight of the polymerizable starch and the polymerizable monomer, and, thereafter, polymerizing the mixture to prepare a polymer gel with microorganism cells enclosed therein.
Claim 6
Dependent Claim
30 words
A polymer gel as in claim 3, wherein the polymerizable monomer is at least one vinyl monomer selected from the group consisting of acrylamide, acrylic acid, hydroxymethylacrylic acid, and N,N'-methylenebisacrylamide.
Claim 7
Dependent Claim
13 words
The process of claim 1 wherein said polymerizing is initiated by a peroxide.
Claim 8
Dependent Claim
14 words
The polymer gel of claim 3 wherein said polymerizing is initiated by a peroxide.
References Cited (Backward Citations)
US4115332A
Cited
US4338401A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-05-22 | 1987-11-21 | 1988-05-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-05-22 | 1991-11-21 | 1992-05-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-05-22 | 1995-11-21 | 1996-05-22 | $7700.00 | Window Closed / Lapsed |