Patent US4448764A successfully refreshed from Google Patents.
US4448764A
Expired / Lapsed
US
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Direct-acting iodinating reagent
Assignee: Pierce Chemical Co
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Filing: 1982-07-06
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Granted: 1984-05-15
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Expires: 2002-07-06
Abstract
Abstract There is disclosed an effective direct-acting iodinating reagent for biological specimens which, because it is direct, avoids the necessity of having present at the same time both an oxidizing agent and sodium iodide. The reagent is a conjugate of an iodous ion and a water insoluble support. The manner of conjugation is such that the ion is complexed onto the surface of the support but is still free to react with groups in proteins susceptible to iodination and thus be transferred to the biological specimen being iodinated. Apart from the advantage of being direct-acting, the reagent has those desirable attributes which are associated with water insoluble oxidants.
Inventors
- 👤 Paul K. Smith
- 👤 Dennis C. Klenk
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 23:52
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/534; A; A61; A61K; A61K51/00; A61K51/02; A61K51/04; A61K51/0489; A61K2123/00; Y; Y10; Y10T; Y10T428/00; Y10T428/29; Y10T428/2982; Y10T428/2991; Y10T428/2998
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
83 words
The process of making a reagent useful for the direct iodination of a biological specimen comprising reacting, in an aqueous medium, sodium iodide with an oxidant comprised of a water insoluble suppot having covalently attached to its surface molecules containing a chloramine group and recovering the support from said aqueous medium, said reaction being such that iodous ions are complexed onto the support but are free to react with groups susceptible to iodination in proteins and be transferred to the specimen being iodinated.
Claim 2
Dependent Claim
83 words
The process of making a reagent useful for the direct iodination of a biological specimen comprising reacting, in an aqueous medium, sodium iodide with an oxidant comprised of a water insoluble suppot having covalently attached to its surface molecules containing a chloramine group and recovering the support from said aqueous medium, said reaction being such that iodous ions are complexed onto the support but are free to react with groups susceptible to iodination in proteins and be transferred to the specimen being iodinated.
Claim 3
Dependent Claim
9 words
The reagent prepared by the process of claim 1.
Claim 4
Dependent Claim
43 words
The reagent of claim 2 wherein the insoluble support is in the form of a non-porous bead having negligible affinity for said specimen and having a size and shape permitting easy physical separation of the reagent from a solution of said biological specimen.
Claim 5
Dependent Claim
22 words
The reagent of claim 3 wherein the bead is spherical and has a diameter of about 0.1 inch to about 0.25 inch.
Claim 6
Dependent Claim
13 words
The reagent of claim 3, 4, or 2 wherein the bead is polystyrene.
Claim 7
Dependent Claim
22 words
The process of claim 1 wherein the oxidant has an oxidizing capacity of at least 1 μeq/in 2 of support surface area.
Claim 8
Dependent Claim
22 words
The process of claim 6 wherein the oxidant has an oxidizing capacity of at least 10 μeq/in 2 of support surface area.
Claim 9
Dependent Claim
32 words
The process of claim 7 wherein the chloramine group of the oxidant is an N-chloroarylsulfonamide group, the support is a non-porous bead, and the bead has negligible affinity for said biological specimen.
Claim 10
Dependent Claim
21 words
The process of claim 1, 6, 7, or 8 wherein the support is polystyrene and the N-chloroarysulfonamide groups are chloramine groups.
Claim 11
Dependent Claim
37 words
The use of the reagent of claim 2 in iodinating a biological specimen comprising contacting, in an aqueous medium, said reagent with the biological specimen to be iodinated and then removing said reagent from said aqueous medium.
Claim 12
Dependent Claim
37 words
The use of the reagent of claim 2 in iodinating a biological specimen comprising contacting, in an aqueous medium, said reagent with the biological specimen to be iodinated and then removing said reagent from said aqueous medium.
Claim 13
Dependent Claim
41 words
The use of the reagent prepared by the process of claim 1 in iodinating a biological specimen comprising contacting, in an aqueous medium, said reagent with the biological specimen to be iodinated and then removing said reagent from said aqueous medium.
Claim 14
Dependent Claim
9 words
The reagent prepared by the process of claim 8.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-05-15 | 1987-11-14 | 1988-05-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-05-15 | 1991-11-14 | 1992-05-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-05-15 | 1995-11-14 | 1996-05-15 | $7700.00 | Window Closed / Lapsed |