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US4446234A
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Vitro cellular interaction with amnion membrane substrate
Assignee: United States Department of Health and Human Services
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Filing: 1981-10-23
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Granted: 1984-05-01
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Expires: 2001-10-23
Abstract
Abstract Amnion membranes derived from mammalian placentas are employed as the substrate tissue in in vitro assay methods for evaluating cellular interaction with natural barrier tissues. The amnion is also employed as a growth substrate, particularly for the culture of cells difficult to culture on conventional substrates. A diagnostic apparatus including an amnion membrane is also disclosed.
Inventors
- 👤 Raimondo Russo
- 👤 Lance A. Liotta
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 23:20
CPC Classifications
A; A61; A61L; A61L27/00; A61L27/36; A61L27/3604; A61L27/38; A61L27/3804; C; C12; C12N; C12N5/00; C12N5/0068; C12N5/06; C12N5/0602; C12N5/0603; C12N5/0605; C12N5/067; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/5005; G01N33/5008; G01N33/5011; G01N33/5091; A61L2430/00; A61L2430/40; C12N2533/00; C12N2533/90; C12N2533/92
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
40 words
In an in vitro assay method for evaluating cellular interaction with natural barrier tissue of the type wherein living cells are interacted in vitro with a substrate tissue, the improvement comprising employing a mammalian amnion membrane as the substrate tissue.
Claim 2
Dependent Claim
40 words
In an in vitro assay method for evaluating cellular interaction with natural barrier tissue of the type wherein living cells are interacted in vitro with a substrate tissue, the improvement comprising employing a mammalian amnion membrane as the substrate tissue.
Claim 3
Dependent Claim
22 words
The invention of claim 1, wherein the cellular interaction to be evaluated comprises cellular invasion or penetration of the natural barrier tissue.
Claim 4
Dependent Claim
14 words
The invention of claim 1, wherein the amnion is derived from a human placenta.
Claim 5
Dependent Claim
24 words
The invention of claim 3, wherein the cells are inflammatory cells and are interacted in the presence of a substance from a biological source.
Claim 6
Dependent Claim
11 words
The invention of claim 4, wherein the cells are polymorphonuclear leucocytes.
Claim 7
Dependent Claim
13 words
The invention of claim 1, wherein the living cells are malignant tumor cells.
Claim 8
Dependent Claim
18 words
The invention of claim 6, wherein the tumor cells are interacted in the presence of a pharmaceutical composition.
Claim 9
Dependent Claim
12 words
The invention of claim 1, wherein the cells are normal migratory cells.
Claim 10
Dependent Claim
18 words
The invention of claims 6 or 8, wherein the cells are interacted in the presence of a chemoattractant.
Claim 11
Dependent Claim
14 words
The invention of claim 1 wherein the amnion connective tissue components are radioactively labeled.
Claim 12
Independent Claim
18 words
A method for culturing cells in vitro comprising interacting living cells with a mammalian amnion membrane growth substrate.
Claim 13
Independent Claim
18 words
A method for culturing cells in vitro comprising interacting living cells with a mammalian amnion membrane growth substrate.
Claim 14
Dependent Claim
22 words
The invention of claim 11, wherein the cells are interacted with the growth substrate in the presence of a serum-free culture medium.
Claim 15
Dependent Claim
10 words
The invention of claim 12, wherein the cells are hepatocytes.
Claim 16
Dependent Claim
30 words
A diagnostic apparatus comprising a chamber including a mammalian amnion membrane dividing the chamber into upper and lower compartments, and support means for supporting the amnion membrane within the chamber.
Claim 17
Dependent Claim
30 words
A diagnostic apparatus comprising a chamber including a mammalian amnion membrane dividing the chamber into upper and lower compartments, and support means for supporting the amnion membrane within the chamber.
Claim 18
Dependent Claim
45 words
The invention of claim 14, wherein the support means comprises an upper ring, a lower ring, and securing means for securing the rings to form said chamber, and wherein the amnion is clamped between said rings to divide the chamber into upper and lower compartments.
Claim 19
Dependent Claim
27 words
The invention of claims 1, 2, 3, 6, 8, 7, 4, 14, or 15, wherein the amnion membrane is wholly or partially denuded of its associated epithelium.
References Cited (Backward Citations)
US4308351A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-05-01 | 1987-10-31 | 1988-05-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-05-01 | 1991-10-31 | 1992-05-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-05-01 | 1995-10-31 | 1996-05-01 | $7700.00 | Window Closed / Lapsed |