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US4446233A
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Homogeneous immunoassay using covalent hybrid antibodies
Assignee: Dade Behring Inc
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Filing: 1982-05-05
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Granted: 1984-05-01
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Expires: 2002-05-05
Abstract
Abstract An immunoassay for analytes such as antigens or haptens, which utilizes covalent hybrid antibodies to modulate the activity of indicators. The hybrid antibody has binding sites for the analyte and the indicator. Final activity of the indicator is proportional to analyte concentration.
Inventors
- 👤 Karen Auditore-Hargreaves
- 👤 Frederick M. Miesowicz
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 17:55
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/68; G01N33/6854; A; A61; A61K; A61K47/00; A61K47/50; A61K47/51; A61K47/68; G01N33/53; G01N33/531; G01N33/577; G01N33/94; Y; Y10; Y10S; Y10S435/00; Y10S435/966; Y10S435/972; Y10S436/00; Y10S436/815; Y10S436/817
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
99 words
A homogeneous immunoassay for the measurement of analyte comprising the steps of incubating sequentially or simultaneously: (A) a substantially pure covalent hybrid antibody consisting essentially of two different heavy chain-light chain half-molecules, wherein the first of said half-molecules provides a binding site for a first antigen and the second of said half-molecules provides a chemically different binding site for the first or a second antigen; wherein said half-molecules are bonded to each other through disulfide linkage; (B) a biological sample containing analyte; (C) an indicator capable of being bound by said hybrid antibody; and (D) reagents for signal-generating reaction.
Claim 2
Dependent Claim
99 words
A homogeneous immunoassay for the measurement of analyte comprising the steps of incubating sequentially or simultaneously: (A) a substantially pure covalent hybrid antibody consisting essentially of two different heavy chain-light chain half-molecules, wherein the first of said half-molecules provides a binding site for a first antigen and the second of said half-molecules provides a chemically different binding site for the first or a second antigen; wherein said half-molecules are bonded to each other through disulfide linkage; (B) a biological sample containing analyte; (C) an indicator capable of being bound by said hybrid antibody; and (D) reagents for signal-generating reaction.
Claim 3
Dependent Claim
21 words
The homogeneous immunoassay of claim 1 wherein the indicator is an enzyme and the reagents for signal-generating reaction include enzyme substrates.
Claim 4
Dependent Claim
21 words
The homogeneous immunoassay of claim 1 wherein the indicator is an aggregating substance and the reagents for signal-generating reaction include polyanalytes.
Claim 5
Dependent Claim
34 words
The homogeneous immunoassay as in one of claims 1-3 wherein the analyte is selected from the group consisting of protein, hormone, drug, vitamin, enzyme, antibody, polysaccharide, bacteria, protozoa, fungi, virus, cell and tissue antigens.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-05-01 | 1987-10-31 | 1988-05-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-05-01 | 1991-10-31 | 1992-05-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-05-01 | 1995-10-31 | 1996-05-01 | $7700.00 | Window Closed / Lapsed |