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US4444317A
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Observation of immunofluorescene for distinguishing between specific and nonspecific binding of conjugates
Assignee: Individual
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Filing: 1981-08-26
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Granted: 1984-04-24
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Expires: 2001-08-26
Abstract
Abstract Disclosed are a method and a device for distinguishing particles or regions therein to which fluorochrome-labeled antibodies or antigens, termed conjugates, are bound by the specific immune reaction of an antibody with its homologous antigen from particles or regions to which the conjugates are nonspecifically bound. The method is based on the different bleaching behavior caused by the different types of binding. Use is made of a microscope whose entire field of view is conventionally illuminated, a central laser beam serving for bleaching analysis.
Inventors
- 👤 Georg Wick
- 👤 Gunther Bock
- 👤 K. Schauenstein
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 11:44
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N33/582; G01N15/00; G01N15/10; G01N15/14; G01N15/1456; G01N15/1459; G01N15/149; G01N21/00; G01N21/62; G01N21/63; G01N21/64; G01N21/6428; G01N21/645; G01N21/6456; G01N21/6458; Y; Y10; Y10S; Y10S209/00; Y10S209/906
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
109 words
A method for distinguishing particles or regions therein to which fluorochrome-labeled antibodies or antigens, termed conjugates, are bound by the specific immune reaction of an antibody with its homologous antigen from particles or regions to which the conjugates are nonspecifically bound, comprising illuminating of the particles or regions with a laser, measuring the resulting fluorescence at three or more moments within the first three milliseconds after the beginning of illumination while illumination continues, comparing the observed bleaching behavior of the particle or region with the characteristic curves of specific and nonspecific binding previously obtained by observation of other particles, and producing a signal indicating the type of binding observed.
Claim 2
Independent Claim
109 words
A method for distinguishing particles or regions therein to which fluorochrome-labeled antibodies or antigens, termed conjugates, are bound by the specific immune reaction of an antibody with its homologous antigen from particles or regions to which the conjugates are nonspecifically bound, comprising illuminating of the particles or regions with a laser, measuring the resulting fluorescence at three or more moments within the first three milliseconds after the beginning of illumination while illumination continues, comparing the observed bleaching behavior of the particle or region with the characteristic curves of specific and nonspecific binding previously obtained by observation of other particles, and producing a signal indicating the type of binding observed.
Claim 3
Dependent Claim
32 words
A method according to claim 1 wherein the particles move at an angle through the laser beam, the signal obtained triggering a sorting mechanism separating specifically bound particles from nonspecifically bound ones.
References Cited (Backward Citations)
US3710933A
Cited
US4081530A
Cited
US4110043A
Cited
US4195930A
Cited
US4173415A
Cited
US4136950A
Cited
US4279345A
Cited
US4407964A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-04-24 | 1987-10-24 | 1988-04-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-04-24 | 1991-10-24 | 1992-04-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-04-24 | 1995-10-24 | 1996-04-24 | $7700.00 | Window Closed / Lapsed |