US4435318A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Electrodialysis preparation of purified AHF concentrate
Assignee: IONICS INCORPORATED 65 GROVE ST WATERTOWN 02172
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Filing: 1981-05-22
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Granted: 1984-03-06
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Expires: 2001-05-22
Abstract
Abstract A process is described for the preparation of a novel protein product of a highly purified antihemophlic factor (AHF) concentrate. The product is prepared by use of a semi-permeable membrane apparatus that desalts the plasma to between 45 to 80% of its salt content while constantly maintaining the plasma at substantially its original starting pH level. Under these conditions the AHF portion of the plasma will precipitate.
Inventors
- 👤 Patrea L. Pabst
- 👤 David H. Bing
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 06:32
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/745; C07K14/755; A; A61; A61K; A61K38/00; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
140 words
A process for the separation and recovery of Factor VIII, von Willebrand's factor, and Factor V from plasma and plasma derivative streams having a pH normally between about 6 to 8.5 comprising removing from said blood stream when present substantially all initial turbidity therein, subsequently passing said blood plasma into and out of an apparatus containing one or more semi-permeable membranes which separate said plasma stream from a salt receiving stream thereby decreasing the salt content of said plasma stream between about 45 to 80% to cause the formation of a protein turbidity enriched in Factor VIII, von Willebrand's factor and Factor V, subsequently removing substantially all of said turbidity and maintaining the temperature of said plasma stream during said separation and recovery process in the range of between about 4°-40° C., and at substantially its original starting pH level.
Claim 2
Dependent Claim
140 words
A process for the separation and recovery of Factor VIII, von Willebrand's factor, and Factor V from plasma and plasma derivative streams having a pH normally between about 6 to 8.5 comprising removing from said blood stream when present substantially all initial turbidity therein, subsequently passing said blood plasma into and out of an apparatus containing one or more semi-permeable membranes which separate said plasma stream from a salt receiving stream thereby decreasing the salt content of said plasma stream between about 45 to 80% to cause the formation of a protein turbidity enriched in Factor VIII, von Willebrand's factor and Factor V, subsequently removing substantially all of said turbidity and maintaining the temperature of said plasma stream during said separation and recovery process in the range of between about 4°-40° C., and at substantially its original starting pH level.
Claim 3
Dependent Claim
16 words
A process according to claim 1 wherein the said plasma stream is anticoagulated using sodium citrate.
Claim 4
Dependent Claim
17 words
A process according to claim 1 wherein the said plasma stream is thawed fresh frozen citrated plasma.
Claim 5
Dependent Claim
18 words
A process according to claim 1 wherein said semi-permeable membrane process for decreasing said salt content comprises electrodialysis.
Claim 6
Dependent Claim
18 words
A process according to claim 1 wherein said semi-permeable membrane process for decreasing said salt content comprises dialysis.
Claim 7
Dependent Claim
19 words
A process according to claim 1 wherein said semi-permeable membrane process for decreasing said salt content comprises ultra filtration.
Claim 8
Dependent Claim
21 words
A process according to claim 1 wherein the original pH of the plasma is maintained substantially constant within ±0.2 pH units.
Claim 9
Dependent Claim
42 words
A process according to claim 1 wherein a vitamin K dependent protein when present in said protein turbidity is selectively removed therefrom by adsorbtion onto a salt selected from the group consisting of barium citrate, barium sulfate and mixtures of the same.
Claim 10
Dependent Claim
15 words
A process according to claim 8 wherein said vitamin K dependent protein is Factor IX.
Claim 11
Dependent Claim
84 words
A process according to claim 1 wherein the recovered protein turbidity comprises between about 0.5 to 2 units of Factor VIII clotting activity per mg of recovered protein, which is equivalent to between about 30 to 50 units per milliliter of protein, vWF antigenic activity in an amount whereby there is a ratio of substantially 1 to 1 vWF antigen activity to units of Factor VIII clotting activity and up to about 2 units of Factor V clotting activity per mg of recovered protein.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-03-06 | 1987-09-05 | 1988-03-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-03-06 | 1991-09-05 | 1992-03-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-03-06 | 1995-09-05 | 1996-03-06 | $7700.00 | Window Closed / Lapsed |