US4276140A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Electrodialysis apparatus and process for fractionating protein mixtures
Assignee: Veolia WTS Systems USA Inc
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Filing: 1980-01-10
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Granted: 1981-06-30
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Expires: 2000-01-10
Abstract
Abstract Apparatus and process for the fractionation of liquid solutions of protein mixtures which includes the steps of subjecting such solutions to electrodialysis (ED) while in the pH range of about between 5-8 until a partial or a substantially complete desalting takes place, the ED preferably being conducted between about 15° C. to 40° C., separating the fraction of protein which precipitates, passing the resulting supernatant salt depleted protein mixture into the concentrating compartments of the ED apparatus whereby the salts entering the salt concentrating chambers from the adjacent salt diluting chambers will substantially restore the original salt content of the desalted protein mixture.
Inventors
- 👤 Surendar M. Jain
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 07:40
CPC Classifications
B; B01; B01D; B01D61/00; B01D61/42; B01D61/44; A; A23; A23C; A23C9/00; A23C9/14; A23C9/144; A23J; A23J1/00; A61; A61M; A61M1/00; A61M1/34; A61M1/3472; A61M1/3482; A61M1/3486; B01D61/46; B01D61/463; C; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/555; B01D2321/00; B01D2321/22; B01D2321/223; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
145 words
A process for fractionating liquid protein mixtures containing dissolved salts, by employing an electrodialysis apparatus having one or more pairs of concentrating and diluting chambers, the chambers being defined by alternating anion and cation exchange membranes located between terminally positioned anode and cathode electrodes, said process comprising passing the said protein mixture into the diluting chambers, impressing a direct current across said electrodes to reduce the salt content of said protein mixture by causing the passage of salt from the diluting chambers into the concentrating chambers, collecting the desalted protein mixture from said diluting chambers, separating and removing one or more protein components from the said desalted protein mixture and thereafter passing the resulting salt depleted mixture into said concentrating chambers whereby said salts entering said concentrating chambers from the adjacent diluting chambers will substantially restore the original salt content of said desalted protein mixture.
Claim 2
Dependent Claim
145 words
A process for fractionating liquid protein mixtures containing dissolved salts, by employing an electrodialysis apparatus having one or more pairs of concentrating and diluting chambers, the chambers being defined by alternating anion and cation exchange membranes located between terminally positioned anode and cathode electrodes, said process comprising passing the said protein mixture into the diluting chambers, impressing a direct current across said electrodes to reduce the salt content of said protein mixture by causing the passage of salt from the diluting chambers into the concentrating chambers, collecting the desalted protein mixture from said diluting chambers, separating and removing one or more protein components from the said desalted protein mixture and thereafter passing the resulting salt depleted mixture into said concentrating chambers whereby said salts entering said concentrating chambers from the adjacent diluting chambers will substantially restore the original salt content of said desalted protein mixture.
Claim 3
Dependent Claim
34 words
The process of claim 1 wherein the liquid the liquid protein mixture is selected from at least one of blood plasma and serum wherein the protein components removed are selected from globulins and euglobulins.
Claim 4
Dependent Claim
179 words
A process for fractionating liquid protein mixtures containing dissolved salts, by employing an electrodialysis apparatus having one or more pairs of concentrating and diluting chambers, the chambers being defined by alternating anion and cation exchange membranes located between terminally positioned anode and cathode electrodes, said process comprising passing the said protein mixture into the diluting chambers, impressing a direct current across said electrodes to reduce the salt content of said protein mixture by causing the passage of salt from the diluting chambers into the concentrating chambers, collecting the desalted protein mixture from said diluting chambers, separating and removing one or more protein components from the said desalted protein mixture, thereafter recycling the resulting salt depleted mixture back into said diluting chambers, and reversing the polarity of the direct current so that the said diluting chambers containing the salt depleted mixture now become salt concentrating chambers and the concentrating chambers containing salts become diluting chambers, whereby said salts will enter said concentrating chamber from the adjacent diluting chambers to substantially restore the original salt content of said desalted protein mixture.
Claim 5
Dependent Claim
179 words
A process for fractionating liquid protein mixtures containing dissolved salts, by employing an electrodialysis apparatus having one or more pairs of concentrating and diluting chambers, the chambers being defined by alternating anion and cation exchange membranes located between terminally positioned anode and cathode electrodes, said process comprising passing the said protein mixture into the diluting chambers, impressing a direct current across said electrodes to reduce the salt content of said protein mixture by causing the passage of salt from the diluting chambers into the concentrating chambers, collecting the desalted protein mixture from said diluting chambers, separating and removing one or more protein components from the said desalted protein mixture, thereafter recycling the resulting salt depleted mixture back into said diluting chambers, and reversing the polarity of the direct current so that the said diluting chambers containing the salt depleted mixture now become salt concentrating chambers and the concentrating chambers containing salts become diluting chambers, whereby said salts will enter said concentrating chamber from the adjacent diluting chambers to substantially restore the original salt content of said desalted protein mixture.
Claim 6
Dependent Claim
33 words
The process of claim 3 wherein the liquid protein mixture is selected from at least one of blood plasma and serum and wherein the protein components removed are selected from globulins and euglobulins.
Claim 7
Dependent Claim
32 words
The process of claims 1 or 3 wherein the liquid protein mixture is whole blood that has been treated with anticoagulants followed by separation of the suspended blood cells prior to desalting.
Claim 8
Independent Claim
154 words
An apparatus for fractionating liquid protein mixtures containing dissolved salts, comprising in combination an electrodialysis apparatus having one or more pairs of concentrating and diluting chambers, the chambers being defined by alternating anion and cation exchange membranes located between terminally positioned anode and cathode electrodes, means for passing the said protein mixture into the diluting chambers, means for impressing a direct current across said electrodes thereby reducing the salt content of said protein mixture by causing the passage of salt from the diluting chambers into the concentrating chambers, means for collecting the desalted protein mixture from said diluting chambers, means for separating and removing one or more protein components from the said desalted protein mixture and conduit means for passing the resulting salt depleted mixture into said concentrating chambers whereby said salts entering said concentrating the chamber from the adjacent diluting chambers will substantially restore the original salt content of said desalted protein mixture.
Claim 9
Independent Claim
154 words
An apparatus for fractionating liquid protein mixtures containing dissolved salts, comprising in combination an electrodialysis apparatus having one or more pairs of concentrating and diluting chambers, the chambers being defined by alternating anion and cation exchange membranes located between terminally positioned anode and cathode electrodes, means for passing the said protein mixture into the diluting chambers, means for impressing a direct current across said electrodes thereby reducing the salt content of said protein mixture by causing the passage of salt from the diluting chambers into the concentrating chambers, means for collecting the desalted protein mixture from said diluting chambers, means for separating and removing one or more protein components from the said desalted protein mixture and conduit means for passing the resulting salt depleted mixture into said concentrating chambers whereby said salts entering said concentrating the chamber from the adjacent diluting chambers will substantially restore the original salt content of said desalted protein mixture.
Claim 10
Independent Claim
189 words
An apparatus for fractionating liquid protein mixtures containing dissolved salts, comprising in combination an electrodialysis apparatus having one or more pairs of concentrating and diluting chambers, the chambers being defined by alternating anion and cation exchange membranes located between terminally positioned anode and cathode electrodes, means for passing the said protein mixture into the diluting chambers, means for impressing a direct current across said electrodes thereby reducing the salt content of said protein mixture by causing the passage of salt from the diluting chambers into the concentrating chambers, means for collecting the desalted protein mixture from said diluting chambers, means for separating and removing one or more protein components from the said desalted protein mixture, conduit means for recycling the resulting salt depleted mixture back into said diluting chambers, and means for reversing the polarity of the direct current so that said diluting chambers containing the salt depleted mixture now become salt concentrating chambers and said concentrating chambers containing salts now become diluting chambers, whereby said salts will enter said concentrating chambers from the adjacent diluting chambers to substantially restore the original salt content of said desalted protein mixture.
Claim 11
Independent Claim
189 words
An apparatus for fractionating liquid protein mixtures containing dissolved salts, comprising in combination an electrodialysis apparatus having one or more pairs of concentrating and diluting chambers, the chambers being defined by alternating anion and cation exchange membranes located between terminally positioned anode and cathode electrodes, means for passing the said protein mixture into the diluting chambers, means for impressing a direct current across said electrodes thereby reducing the salt content of said protein mixture by causing the passage of salt from the diluting chambers into the concentrating chambers, means for collecting the desalted protein mixture from said diluting chambers, means for separating and removing one or more protein components from the said desalted protein mixture, conduit means for recycling the resulting salt depleted mixture back into said diluting chambers, and means for reversing the polarity of the direct current so that said diluting chambers containing the salt depleted mixture now become salt concentrating chambers and said concentrating chambers containing salts now become diluting chambers, whereby said salts will enter said concentrating chambers from the adjacent diluting chambers to substantially restore the original salt content of said desalted protein mixture.
Claim 12
Independent Claim
39 words
The apparatus of claims 5 or 7, the liquid protein mixture is whole blood and wherein means for anticoagulating and thereafter separating the suspended blood cells are provided prior to passing the said protein mixture into the diluting chamber.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-06-29 | 1984-12-29 | 1985-06-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-06-29 | 1988-12-29 | 1989-06-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-06-29 | 1992-12-29 | 1993-06-30 | $7700.00 | Window Closed / Lapsed |