US4434156A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Monoclonal antibodies specific for the human transferrin receptor glycoprotein
Assignee: Salk Institute for Biological Studies
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Filing: 1981-10-26
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Granted: 1984-02-28
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Expires: 2001-10-26
Abstract
Abstract Monoclonal antibodies are produced specific for the cell surface transferrin receptor of human cells. Animals are inoculated with purified human transferrin receptor glycoprotein, human hematopoietic cells or fragments thereof, and spleen cells obtained from the animals are fused with myeloma cells to produce hybridomas. The hybridomas are cultured as clones, and antibodies obtained from the individual clones are tested for their specificty for human transferrin receptor. Clones which produce antibodies specific for the receptor and that interfere with or block transferrin binding are selected for further culturing to produce the antibody, and the antibody is obtained from the culture growth medium or from ascitic fluid of an animal bearing a tumor of the hybridoma. Monoclonal antibodies which block transferrin binding are useful in regulating cell growth and for other therapeutic uses.
Inventors
- 👤 Ian S. Trowbridge
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 16:56
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/18; C07K16/28; C07K16/2881; A; A61; A61P; A61P35/00; A61K; A61K38/00; C07K2317/00; C07K2317/70; C07K2317/73
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
90 words
A method of producing monoclonal antibodies specific for the transferrin binding site of human transferrin receptor glycoprotein and which block binding of transferrin to the glycoprotein, the method comprising inoculating a rodent animal with material containing said transferrin receptor glycoprotein, obtaining antibody-producing cells from said animal, fusing said antibody-producing cells and neoplastic cells to produce hybridomas, selecting hybridomas that produce an antibody which reacts with said transferrin binding site and blocks subsequent binding of transferrin to the glycoprotein, producing clones of said selected hybridomas, and collecting the antibodies produced thereby.
Claim 2
Independent Claim
90 words
A method of producing monoclonal antibodies specific for the transferrin binding site of human transferrin receptor glycoprotein and which block binding of transferrin to the glycoprotein, the method comprising inoculating a rodent animal with material containing said transferrin receptor glycoprotein, obtaining antibody-producing cells from said animal, fusing said antibody-producing cells and neoplastic cells to produce hybridomas, selecting hybridomas that produce an antibody which reacts with said transferrin binding site and blocks subsequent binding of transferrin to the glycoprotein, producing clones of said selected hybridomas, and collecting the antibodies produced thereby.
Claim 3
Dependent Claim
15 words
A method according to claim 1 wherein said antibody-producing cells are BALB/C mouse spleen cells.
Claim 4
Dependent Claim
26 words
A method according to claim 1 wherein said transferrin receptor glycoprotein is obtained from the group of human hematopoietic cells and human hematopoietic cell membrane material.
Claim 5
Dependent Claim
14 words
A method according to claim 3 wherein said human hematopoietic cell line is CCRF-CEM.
Claim 6
Dependent Claim
20 words
A method according to claim 1 wherein said neoplastic tissue cells are of the non producer myeloma cell line, S194/5.XXO.BU.1.
Claim 7
Dependent Claim
35 words
A method according to claim 1 wherein said clones are grown in host animals, and said antibody is obtained from the group consisting of the host animal's blood serum and the host animal's ascitic fluid.
Claim 8
Dependent Claim
44 words
A cell line produced by the fusion of an antibody-producing rodent animal cell and a neoplastic cell, which produces a rodent antibody specific for the transferrin binding site of the human transferrin receptor glycoprotein and which blocks binding of transferrin to the receptor glycoprotein.
Claim 9
Dependent Claim
44 words
A cell line produced by the fusion of an antibody-producing rodent animal cell and a neoplastic cell, which produces a rodent antibody specific for the transferrin binding site of the human transferrin receptor glycoprotein and which blocks binding of transferrin to the receptor glycoprotein.
Claim 10
Dependent Claim
17 words
A cell line according to claim 7 wherein said antibody-producing cell is derived from the murine genus.
Claim 11
Dependent Claim
25 words
A cell line according to claim 7 wherein said antibody-producing cells are selected from the group of murine spleen cells and murine lymph node cells.
Claim 12
Dependent Claim
16 words
A cell line according to claim 7 wherein said antibody-producing cells are BALB/c mouse spleen cells.
Claim 13
Dependent Claim
15 words
A cell line according to claim 7 wherein said neoplastic cell is non antibody producing.
Claim 14
Dependent Claim
19 words
A cell line according to claim 7 selected from the group consisting of HB-8094 hybridoma and antibody-producing reclones thereof.
Claim 15
Dependent Claim
24 words
Rodent monoclonal antibodies specific for the transferrin binding site of human transferrin receptor glycoprotein and which block binding of transferrin to the receptor glycoprotein.
Claim 16
Dependent Claim
24 words
Rodent monoclonal antibodies specific for the transferrin binding site of human transferrin receptor glycoprotein and which block binding of transferrin to the receptor glycoprotein.
Claim 17
Dependent Claim
21 words
Monoclonal antibodies according to claim 13 obtained from cultures selected from the group consisting of HB-8094 hybridoma and antibody-producing reclones thereof.
Claim 18
Independent Claim
41 words
A method of regulating cell growth in a mammal comprising administering a therapeutically effective does of a rodent monoclonal antibody that is specific to the transferrin binding site of human transferrin receptor glycoprotein and blocks transferrin binding to the receptor glycoprotein.
Claim 19
Independent Claim
41 words
A method of regulating cell growth in a mammal comprising administering a therapeutically effective does of a rodent monoclonal antibody that is specific to the transferrin binding site of human transferrin receptor glycoprotein and blocks transferrin binding to the receptor glycoprotein.
Claim 20
Dependent Claim
18 words
A method according to claim 15 wherein said antibody is obtained from HB-8094 hybridoma or antibody-producing reclones thereof.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-02-27 | 1987-08-29 | 1988-02-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-02-27 | 1991-08-29 | 1992-02-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-02-27 | 1995-08-29 | 1996-02-28 | $7700.00 | Window Closed / Lapsed |