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Patent US4433051A successfully refreshed from Google Patents.
US4433051A
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Derivatives of α-difluoromethylornithine useful in analysis
Assignee: Agfa Gevaert AG
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Filing: 1981-10-14
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Granted: 1984-02-21
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Expires: 2001-10-14
Abstract
Abstract There are provided novel labelled derivatives, namely α-difluoromethylornithine tagged with rhodamine or with biotin. These are of value in analysis and in the cytochemical localization of enzymes.
Inventors
- 👤 Gad Gilad
- 👤 Varda Gilad
- 👤 Meir Wilchek
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 15:40
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/533; G01N2474/00; G01N2474/20; Y; Y10; Y10S; Y10S435/00; Y10S435/968
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
20 words
A labelled derivative of α-difluoromethylornithine (α-dFMO), namely α-dFMO tagged with rhodamine B or with biotin, respectively, of the formulas ##STR3##
Claim 2
Dependent Claim
20 words
A labelled derivative of α-difluoromethylornithine (α-dFMO), namely α-dFMO tagged with rhodamine B or with biotin, respectively, of the formulas ##STR3##
Claim 3
Dependent Claim
51 words
A process for tagging α-dFMO with rhodamine B which comprises dissolving the inhibitor in water, reacting same with an excess of cupric carbonate, adding rhodamine-B-isothiocyanate to the inhibitor-copper chelate at a pH of about 8.4, acidifying to pH 3, washing and lyophilizing the precipitate, dissolving same in Tris-HCl buffer, pH 7.2.
Claim 4
Dependent Claim
51 words
A process for tagging α-dFMO with rhodamine B which comprises dissolving the inhibitor in water, reacting same with an excess of cupric carbonate, adding rhodamine-B-isothiocyanate to the inhibitor-copper chelate at a pH of about 8.4, acidifying to pH 3, washing and lyophilizing the precipitate, dissolving same in Tris-HCl buffer, pH 7.2.
Claim 5
Dependent Claim
50 words
A process for preparing α-dFMO coupled to biotin which comprises preparing an enzyme-copper chelate as defined in claim 2, dissolving biotinyl-N-hydroxysuccinimide in a suitable solvent and admixing these two components in a carbonate/bicarbonate buffer pH 8.6, decanting after an adequate period of reaction, adding hydrogen sulfide and lyophilizing the precipitate.
Claim 6
Dependent Claim
71 words
A process for the cytochemical localization of ornithine decarboxylase (ODC) activity comprising: subjecting tissue samples to the action of an enzyme activated irreversible inhibitor of ODC which inhibitor has been labelled with specific molecules which render it visible by light or fluorescence microscopy; and visually localizing the labelled inhibitor by light or fluorescence microscopy, wherein said enzyme activated irreversible inhibitor of ODC is an α-dFMO derivative in accordance with claim 1.
References Cited (Backward Citations)
US2937186A
Cited
US4134792A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-02-20 | 1987-08-22 | 1988-02-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-02-20 | 1991-08-22 | 1992-02-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-02-20 | 1995-08-22 | 1996-02-21 | $7700.00 | Window Closed / Lapsed |