Patent US4431737A successfully refreshed from Google Patents.
US4431737A
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Process for the production of alpha-galactosidase and uses of the enzyme thus obtained
Assignee: ENICHEM SYNTHESIS SpA AN ITALIAN Co
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Filing: 1982-11-30
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Granted: 1984-02-14
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Expires: 2002-11-30
Abstract
Abstract The enzyme alpha-galactosidase is prepared from a constitutive mutant of the strain Saccharomyces cerevisiae NRRL-Y-12057, said mutant being marked by the identification number NRRL-Y-12533.
Inventors
- 👤 Roberto Olivieri
- 👤 Paolo Pansolli
- 👤 Eugenio Fascetti
- 👤 Pierluigi Ciuffolotti
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 08:25
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2465; A; A23; A23L; A23L11/00; A23L11/30; A23L11/33; C12N1/00; C12N1/14; C12N1/16; C12N1/18; C12N1/185; C12R; C12R2001/00; C12R2001/645; C12R2001/85; C12R2001/865; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/911; Y10S435/94; Y10S435/942
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
21 words
A biologically pure culture of Saccharomyces cerevisiae NNRL-Y 12533, said culture being capable of producing alpha-galactosidase in the absence of galactose.
Claim 2
Dependent Claim
21 words
A biologically pure culture of Saccharomyces cerevisiae NNRL-Y 12533, said culture being capable of producing alpha-galactosidase in the absence of galactose.
Claim 3
Dependent Claim
35 words
A method for the preparation of the microorganism claimed in claim 1, consisting in subjecting to the action of ultraviolet radiations the microorganism of the Saccharomyces cerevisiae genus, variety oleaginosus identified by the symbol NRRL-Y-12057.
Claim 4
Dependent Claim
29 words
Method for the preparation of alpha-galactosidase comprising the step of culturing the Saccharomyces cerevisiae NRRL-Y-12533 strain in a culturing medium containing enzyme-inducing substances and recovering the alpha-galactosidase thus produced.
Claim 5
Dependent Claim
29 words
Method for the preparation of alpha-galactosidase comprising the step of culturing the Saccharomyces cerevisiae NRRL-Y-12533 strain in a culturing medium containing enzyme-inducing substances and recovering the alpha-galactosidase thus produced.
Claim 6
Dependent Claim
21 words
Method according to claim 3, wherein the inducing substances are members selected from the group consisting of glucose, melibiose and galactose.
Claim 7
Dependent Claim
20 words
Method according to claim 3, wherein the fermentation is carried out in a pH range of from 4 to 7.
Claim 8
Dependent Claim
23 words
Method according to claim 3, characterized in that the fermentation is carried out at a temperature comprised between 20° C. and 40° C.
Claim 9
Dependent Claim
33 words
Method for the preparation of alpha-galactosidase comprising the step of culturing strain NRRL-Y-12533 of Saccharomyces cerevisiae in a culturing medium that is free of an inductor and thereafter recovering the alpha-galactosidase thus produced.
Claim 10
Dependent Claim
33 words
Method for the preparation of alpha-galactosidase comprising the step of culturing strain NRRL-Y-12533 of Saccharomyces cerevisiae in a culturing medium that is free of an inductor and thereafter recovering the alpha-galactosidase thus produced.
Claim 11
Dependent Claim
8 words
A biologically pure culture of Saccharomyces cerevisiae NRRL-Y-12533.
Claim 12
Dependent Claim
8 words
A biologically pure culture of Saccharomyces cerevisiae NRRL-Y-12533.
References Cited (Backward Citations)
US4008334A
Cited
US4216235A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-02-13 | 1987-08-15 | 1988-02-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-02-13 | 1991-08-15 | 1992-02-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-02-13 | 1995-08-15 | 1996-02-14 | $7700.00 | Window Closed / Lapsed |