Patent US4431507A successfully refreshed from Google Patents.
US4431507A
Expired / Lapsed
US
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Enzyme electrode
Assignee: Panasonic Holdings Corp
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Filing: 1982-01-12
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Granted: 1984-02-14
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Expires: 2002-01-12
Abstract
Abstract The disclosure is directed to an improved enzyme electrode which includes a first electrode having at least one kind of an enzyme immobilized on it for electrochemically detecting a substance to be produced in association with a reaction based on the enzyme, and a second electrode for electrochemically removing materials which interfere with the detection by the first electrode. The second electrode is disposed at the side of a test solution containing a substrate of the enzyme with respect to the first electrode.
Inventors
- 👤 Shiro Nankai
- 👤 Akihiro Imai
- 👤 Takashi Iijima
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 18:28
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
74 words
An enzyme electrode assembly which comprises a first electrode including at least one enzyme immobilized thereon for electrochemically detecting a substance to be produced in association with a reaction based on said enzyme, and a second electrode for electrochemically removing materials which interfere with said detection by said first electrode, said second electrode being disposed at the side of a test solution containing a substrate of said enzyme with respect to said first electrode.
Claim 2
Dependent Claim
74 words
An enzyme electrode assembly which comprises a first electrode including at least one enzyme immobilized thereon for electrochemically detecting a substance to be produced in association with a reaction based on said enzyme, and a second electrode for electrochemically removing materials which interfere with said detection by said first electrode, said second electrode being disposed at the side of a test solution containing a substrate of said enzyme with respect to said first electrode.
Claim 3
Dependent Claim
31 words
An enzyme electrode assembly as claimed in claim 1, wherein said second electrode is mode of a thin layer formed selectively of a metal or metal oxide on a porous membrane.
Claim 4
Dependent Claim
45 words
An enzyme electrode assembly as claimed in claim 1 or 2, wherein said first electrode is made of by a porous membrane having a thin layer of a metal or metal oxide formed on its surface, with the enzyme being immobilized on said porous membrane.
Claim 5
Dependent Claim
21 words
An enzyme electrode assembly as claimed in claim 3, wherein said first electrode and second electrode are laminated one upon another.
Claim 6
Dependent Claim
36 words
An enzyme electrode assembly as claimed in claim 3, wherein said first electrode is formed at one side of the porous membrane, with said second electrode being formed at the other side of said porous membrane.
Claim 7
Dependent Claim
145 words
An electrode assembly comprising a first electrode including at least one enzyme immobilized thereon for electrochemically detecting a substance to be produced in association with a reaction based on said enzyme, said enzyme electrode being made of a porous membrane having a thin layer of a metal or metal oxide layer on one surface and at least one enzyme immobilized on its opposite surface and a second electrode for electrochemically removing materials which interfere with said detection by said first electrode, said second electrode being also composed of a porous membrane with a thin layer of a metal or metal oxide on one side of the porous membrane; said first and second electrodes being laminated such that the immobilized enzyme(s) is sandwiched between between the two porous membranes and the metal or metal oxide layers are located on the outer portions of the laminated structure.
Claim 8
Dependent Claim
145 words
An electrode assembly comprising a first electrode including at least one enzyme immobilized thereon for electrochemically detecting a substance to be produced in association with a reaction based on said enzyme, said enzyme electrode being made of a porous membrane having a thin layer of a metal or metal oxide layer on one surface and at least one enzyme immobilized on its opposite surface and a second electrode for electrochemically removing materials which interfere with said detection by said first electrode, said second electrode being also composed of a porous membrane with a thin layer of a metal or metal oxide on one side of the porous membrane; said first and second electrodes being laminated such that the immobilized enzyme(s) is sandwiched between between the two porous membranes and the metal or metal oxide layers are located on the outer portions of the laminated structure.
Claim 9
Dependent Claim
95 words
An electrode assembly comprising a porous membrane having first a metal or metal oxide electrode layer located on one side with an enzyme immobilized on said layer and within the pores of the membrane to constitute a first electrode and a second electrode metal and metal oxide layer located in the opposite side of the porous membrane, said first electrode-functioning to detect a substance to be produced in association with an enzyme reaction, based on said enzyme and said second electrode functioning to electrochemically remove materials which interfere with said detection by the first electrode.
Claim 10
Dependent Claim
95 words
An electrode assembly comprising a porous membrane having first a metal or metal oxide electrode layer located on one side with an enzyme immobilized on said layer and within the pores of the membrane to constitute a first electrode and a second electrode metal and metal oxide layer located in the opposite side of the porous membrane, said first electrode-functioning to detect a substance to be produced in association with an enzyme reaction, based on said enzyme and said second electrode functioning to electrochemically remove materials which interfere with said detection by the first electrode.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-02-13 | 1987-08-15 | 1988-02-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-02-13 | 1991-08-15 | 1992-02-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-02-13 | 1995-08-15 | 1996-02-14 | $7700.00 | Window Closed / Lapsed |