Patent US4430496A successfully refreshed from Google Patents.
US4430496A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Strong anion exchange composition and methods
Assignee: Varian Inc
•
Filing: 1982-03-16
•
Granted: 1984-02-07
•
Expires: 2002-03-16
Abstract
Abstract A strong anion exchange composition useful in monophosphate nucleotide isomer separations is comprised of an inert porous particle having a silyl alkyl ammonium compound fixedly attached by a covalent silicon-oxygen-silicon bond to the surface thereof. A process for synthesizing the strong anion exchange composition in an aqueous solvent is disclosed together with methods for separation of monophosphate nucleotide isomers, highly phophorylated nucleotides and aldopentose carbohydrates using a column packed with this strong anion exchange composition.
Inventors
- 👤 Seth R. Abbott
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 18:57
CPC Classifications
B; B01; B01J; B01J41/00; B01J41/08; B01J41/10; B01J20/00; B01J20/22; B01J20/26; B01J20/264; B01J20/265; B01J20/267; B01J20/28; B01J20/28002; B01J20/28004; B01J20/28054; B01J20/28057; B01J20/28078; B01J20/28083; B01J20/281; B01J20/286; B01J20/30; B01J20/32; B01J20/3202; B01J20/3204; B01J20/3206; B01J20/3208; B01J20/321; B01J20/3212; B01J20/3214; B01J20/3217; B01J20/3231; B01J20/3242; B01J20/3244; B01J20/3246; B01J20/3257; B01J41/04; B01J41/05; B01J41/09; B01J41/20; B01J2220/00; B01J2220/50; B01J2220/54; B01J2220/58
Claims Analysis
Total Claims: 33
Claim 1
Independent Claim
97 words
A method for the separation of components contained in a biological composition, the steps of the method comprising: (a) packing a column with a strong anion exchange composition comprising an inert porous particle having a particle size of about 3 to about 20 microns which has covalently attached to the silica surface a silyl alkyl ammonium compound having the general formula: ##STR4## wherein R is methyl or ethyl; n is an integer ranging from 2 to 5; and X is an exchangeable anion; (b) passing said biological composition through said column; and, (c) recovering the separated components.
Claim 2
Independent Claim
97 words
A method for the separation of components contained in a biological composition, the steps of the method comprising: (a) packing a column with a strong anion exchange composition comprising an inert porous particle having a particle size of about 3 to about 20 microns which has covalently attached to the silica surface a silyl alkyl ammonium compound having the general formula: ##STR4## wherein R is methyl or ethyl; n is an integer ranging from 2 to 5; and X is an exchangeable anion; (b) passing said biological composition through said column; and, (c) recovering the separated components.
Claim 3
Dependent Claim
14 words
The method of claim 1 wherein said column is a high-pressure liquid chromatography column.
Claim 4
Dependent Claim
32 words
The method of claim 2 wherein the particle of the strong anion exchange composition is selected from a group consisting of microparticulate silica, alumina, a cross-linked dextran, and a cross-linked polystyrene-divinylbenzene resin.
Claim 5
Dependent Claim
20 words
The method of claim 3 wherein the particle of which said strong anion exchange composition is comprised is microparticulate silica.
Claim 6
Dependent Claim
16 words
The method of claim 4 wherein said silica is a high-pressure liquid chromatography grade silica gel.
Claim 7
Dependent Claim
34 words
The method of claim 2 wherein the said biological composition separated by said strong anion exchange composition is comprised of members of a group consisting of nucleic acid components, amino acids, and dicarboxylic acids.
Claim 8
Dependent Claim
24 words
The method of claim 6 wherein the said biological composition separated by said strong anion exchange composition is a mixture of nucleic acid components.
Claim 9
Dependent Claim
13 words
The method of claim 1 wherein said particle size is about 3-10 microns.
Claim 10
Dependent Claim
9 words
The method of claim 1 wherein n is 3.
Claim 11
Dependent Claim
9 words
The method of claim 9 wherein R is methyl.
Claim 12
Dependent Claim
13 words
The method of claim 1 wherein said ammonium compound is N-trimethoxy silyl-propyl-N,N,N-trimethylammonium chloride.
Claim 13
Dependent Claim
99 words
A liquid chromatographic column comprising an elongated tube, a strong anion exchange composition packed into said tube, said strong anion exchange composition comprising an inert porous particle having a size of about 5 to 20 microns and having attached to the surface of said inert porous particle by covalent bonding a silyl alkyl ammonium compound having the general formula: ##STR5## wherein R is methyl or ethyl; n is an integer ranging from 2 to 5; and X is an exchangeable anion, said strong anion exchange composition being capable of simultaneous separation of mixtures of polyfunctional compounds and isomers thereof.
Claim 14
Dependent Claim
99 words
A liquid chromatographic column comprising an elongated tube, a strong anion exchange composition packed into said tube, said strong anion exchange composition comprising an inert porous particle having a size of about 5 to 20 microns and having attached to the surface of said inert porous particle by covalent bonding a silyl alkyl ammonium compound having the general formula: ##STR5## wherein R is methyl or ethyl; n is an integer ranging from 2 to 5; and X is an exchangeable anion, said strong anion exchange composition being capable of simultaneous separation of mixtures of polyfunctional compounds and isomers thereof.
Claim 15
Dependent Claim
14 words
The column of claim 12 wherein said column is a high-pressure liquid chromatography column.
Claim 16
Dependent Claim
32 words
The column of claim 13 wherein the particle of the strong anion exchange composition is selected from a group consisting of microparticulate silica, alumina, a cross-linked dextran, and a cross-linked polystyrene-divinylbenzene resin.
Claim 17
Dependent Claim
20 words
The column of claim 14 wherein the particle of which said strong anion exchange composition is comprised is microparticulate silica.
Claim 18
Dependent Claim
16 words
The column of claim 15 wherein said silica is a high-pressure liquid chromatography grade silica gel.
Claim 19
Dependent Claim
13 words
The column of claim 14 wherein said particle size is about 5-10 microns.
Claim 20
Dependent Claim
13 words
The column of claim 12 wherein n is 3 and R is methyl.
Claim 21
Dependent Claim
13 words
The column of claim 12 wherein said ammonium compound is N-trimethoxy silyl-propyl-N,N,N-trimethylammonium chloride.
Claim 22
Independent Claim
129 words
A method for performing liquid chromatography comprising placing a solution of a composition comprising a mixture of polyfunctional compounds and isomers thereof on the head of a chromatographic column, flowing a solvent system through said chromatographic column, wherein said chromatographic column is packed with a packing material comprising a strong anion exchange composition, said strong anion exchange composition comprising an inert porous particle having a size from about 3 to 20 microns which has covalently attached to its surface a silyl alkyl ammonium compound having the general formula: ##STR6## wherein R is methyl or ethyl; n is an integer ranging from 2 to 5; and X is an exchangeable anion; wherein said strong anion exchange composition is capable of simultaneous separation of mixtures of polyfunctional compounds and isomers thereof.
Claim 23
Independent Claim
129 words
A method for performing liquid chromatography comprising placing a solution of a composition comprising a mixture of polyfunctional compounds and isomers thereof on the head of a chromatographic column, flowing a solvent system through said chromatographic column, wherein said chromatographic column is packed with a packing material comprising a strong anion exchange composition, said strong anion exchange composition comprising an inert porous particle having a size from about 3 to 20 microns which has covalently attached to its surface a silyl alkyl ammonium compound having the general formula: ##STR6## wherein R is methyl or ethyl; n is an integer ranging from 2 to 5; and X is an exchangeable anion; wherein said strong anion exchange composition is capable of simultaneous separation of mixtures of polyfunctional compounds and isomers thereof.
Claim 24
Dependent Claim
14 words
The method of claim 20 wherein said column is a high-pressure liquid chromatography column.
Claim 25
Dependent Claim
32 words
The method of claim 21 wherein the particle of the strong anion exchange composition is selected from a group consisting of microparticulate silica, alumina, a cross-linked dextran, and a cross-linked polystyrene-divinylbenzene resin.
Claim 26
Dependent Claim
19 words
The method of claim 22 wherein the particle of said strong anion exchange composition is comprised is microparticulate silica.
Claim 27
Dependent Claim
16 words
The method of claim 23 wherein said silica is a high-pressure liquid chromatography grade silica gel.
Claim 28
Independent Claim
98 words
A method for the separation of nucleotide isomers on a chromatographic column, the steps of the method comprising: (a) packing a column with a strong anion exchange composition comprising a microparticulate silica having a particle size of about 3 to about 20 microns which has covalently attached to the silica surface a silyl alkyl ammonium compound having the general formula: ##STR7## wherein R is methyl or ethyl; n is an integer ranging from 2 to 5; and X is an exchangeable anion; (b) passing said nucleotide isomer composition through said chromatographic column; and (c) recovering the separated components.
Claim 29
Independent Claim
98 words
A method for the separation of nucleotide isomers on a chromatographic column, the steps of the method comprising: (a) packing a column with a strong anion exchange composition comprising a microparticulate silica having a particle size of about 3 to about 20 microns which has covalently attached to the silica surface a silyl alkyl ammonium compound having the general formula: ##STR7## wherein R is methyl or ethyl; n is an integer ranging from 2 to 5; and X is an exchangeable anion; (b) passing said nucleotide isomer composition through said chromatographic column; and (c) recovering the separated components.
Claim 30
Dependent Claim
34 words
The method of claim 25 wherein the said nucleotide isomers separated by said strong anion exchange composition are comprised of members of a group consisting of nucleic acid components, amino acids, and dicarboxylic acids.
Claim 31
Dependent Claim
17 words
The method of claim 26 wherein the said nucleotide isomers comprise a mixture of nucleic acid components.
Claim 32
Dependent Claim
13 words
The method of claim 25 wherein n is 3 and R is methyl.
Claim 33
Dependent Claim
13 words
The method of claim 25 wherein said ammonium compound is N-trimethoxy silyl-propyl-N,N,N-trimethylammonium chloride.
References Cited (Backward Citations)
US4290892A
Cited
US4340496A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-02-06 | 1987-08-08 | 1988-02-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-02-06 | 1991-08-08 | 1992-02-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-02-06 | 1995-08-08 | 1996-02-07 | $7700.00 | Window Closed / Lapsed |