Patent US4430437A successfully refreshed from Google Patents.
US4430437A
Expired / Lapsed
US
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Test methods employing monoclonal antibodies against Herpes simplex virus types 1 and 2 nucleocapsids proteins
Assignee: United States Department of Health and Human Services
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Filing: 1980-08-27
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Granted: 1984-02-07
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Expires: 2000-08-27
Abstract
Abstract The method of producing clinical assays for use of monoclonal antibodies in the diagnosis of Herpes simplex virus (HSV) infections and the differentiation of Herpes Simplex virus types 1 and 2 as a diagnostic kit for differentiating HSV-1 and HSV-2 utilizing clone 1D4 against HSV-1 and clone 3E1 against HSV-2.
Inventors
- 👤 Berge Hampar
- 👤 Martin Zweig
- 👤 Harvey Rabin
- 👤 Conrad J. Heilman, Jr.
- 👤 Ralph F. Hopkins, III
- 👤 Russell H. Neubauer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 22:59
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/56983; G01N33/56994; Y; Y10; Y10S; Y10S435/00; Y10S435/975
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
61 words
In a method of clinical testing and differentiating antibodies to Herpes simplex virus, HSV-1 and HSV-2, employing a competition immunoprecipitation, test, the improvement which comprises employing in said test as reagents monoclonal antibodies to the respective viruses HSV-1 and HSV-2 nucleocapsid p. 40 and p. 45 proteins of which antibodies are produced by hybrid cell lines ID4 (HSV-1) and 3EI (HSV-2).
Claim 2
Dependent Claim
61 words
In a method of clinical testing and differentiating antibodies to Herpes simplex virus, HSV-1 and HSV-2, employing a competition immunoprecipitation, test, the improvement which comprises employing in said test as reagents monoclonal antibodies to the respective viruses HSV-1 and HSV-2 nucleocapsid p. 40 and p. 45 proteins of which antibodies are produced by hybrid cell lines ID4 (HSV-1) and 3EI (HSV-2).
Claim 3
Dependent Claim
12 words
The method of claim 1 wherein the test is competition radioimmuno precipitation.
Claim 4
Dependent Claim
59 words
In a method of clinical testing and differentiating antibodies to Herpes simplex virus, HSV-1 and HSV-2, employing the enzyme-linked immunoabsorbent assay, the improvement which comprises employing in said test as reagents monoclonal antibodies to the respective viruses HSV-1 and HSV-2 nucleocapsid p. 40 and p. 45 proteins which antibodies are produced by hybrid cell lines ID- (HSV-1) and 3EI(HSV-2).
Claim 5
Dependent Claim
59 words
In a method of clinical testing and differentiating antibodies to Herpes simplex virus, HSV-1 and HSV-2, employing the enzyme-linked immunoabsorbent assay, the improvement which comprises employing in said test as reagents monoclonal antibodies to the respective viruses HSV-1 and HSV-2 nucleocapsid p. 40 and p. 45 proteins which antibodies are produced by hybrid cell lines ID- (HSV-1) and 3EI(HSV-2).
Claim 6
Dependent Claim
10 words
The method of claim 3 wherein the enzyme is peroxidase.
Claim 7
Independent Claim
56 words
The method of testing and differentiating Herpes simplex virus, HSV-1 and HSV-2, in a test sample which comprises reacting the infected samples with monoclonal antibodies against the nucleocapsid p. 40 and p. 45 proteins of HSV-1 and HSV-2, respectively, and noting the reaction thereof by immuno-peroxidase assay, immunofluorescence assay, enzyme-linked immunoabsorbent assay immunoprecipitation assay, or radioimmunoassay.
Claim 8
Independent Claim
56 words
The method of testing and differentiating Herpes simplex virus, HSV-1 and HSV-2, in a test sample which comprises reacting the infected samples with monoclonal antibodies against the nucleocapsid p. 40 and p. 45 proteins of HSV-1 and HSV-2, respectively, and noting the reaction thereof by immuno-peroxidase assay, immunofluorescence assay, enzyme-linked immunoabsorbent assay immunoprecipitation assay, or radioimmunoassay.
Claim 9
Dependent Claim
12 words
The method of claim 5 wherein the test is an immunoperoxidase assay.
Claim 10
Dependent Claim
11 words
The method of claim 5 wherein the test is an radioimmunoassay.
Claim 11
Dependent Claim
13 words
The method of claim 5 wherein the test is an enzyme-linked immunoabsorbent assay.
Claim 12
Dependent Claim
12 words
The method of claim 5 wherein the test is an immunofluorescence assay.
Claim 13
Dependent Claim
12 words
The method of claim 5 wherein the test is an immunoprecipitation assay.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-02-06 | 1987-08-08 | 1988-02-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-02-06 | 1991-08-08 | 1992-02-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-02-06 | 1995-08-08 | 1996-02-07 | $7700.00 | Window Closed / Lapsed |