Abstract Methods featuring, in one aspect, a method of preparing an antigen or mixture of antigens substantially free of antigens specific to at least two monoclonal antibodies, said method involving contacting an antigen mixture with one or more previously-isolated monoclonal antibodies to form complexes between those antibodies and antigens present in the mixture specific to the antibodies, removing the complexes from the antigen mixture to yield a partially purified antigen mixture, immunizing an animal with the partially purified antigen mixture, fusing spleen cells from the immunized animal to myeloma cells to form hybridomas capable of producing additional monoclonal antibodies, culturing said hybridomas to produce said additional monoclonal antibodies, contacting a sample of the partially purified antigen mixture with said additional monoclonal antibodies to form complexes between said additional monoclonal antibodies and antigens present in the antigen mixture specific to the additional monoclonal antibodies, and removing the complexes from the antigen mixture.
A method of preparing one or more monoclonal antibodies, said method comprising the steps of (a) providing a cell-free liquid containing a plurality of different antigens, (b) contacting a sample of said liquid with one or more monoclonal antibodies each specific to a said antigen to form complexes between said monoclonal antibodies and antigens present in said liquid and then removing said complexes from said liquid to yield a partially purified liquid substantially free of antigens specific to said one or more monoclonal antibodies, (c) immunizing an animal with said partially purified liquid, (d) fusing spleen cells from said immunized animal to myeloma cells to form hybridomas capable of producing monoclonal antibodies, (e) culturing said hybridomas to produce said monoclonal antibodies, (f) isolating one or more of said monoclonal antibodies, (g) repeating steps (b) through (f) in sequence, at least once, using in step (b) at least one monoclonal antibody of step (f) in the previous sequence, and (h) terminating said method when, or before, said liquid contains one antigen.
Claim 2
Independent Claim
170 words
A method of preparing one or more monoclonal antibodies, said method comprising the steps of (a) providing a cell-free liquid containing a plurality of different antigens, (b) contacting a sample of said liquid with one or more monoclonal antibodies each specific to a said antigen to form complexes between said monoclonal antibodies and antigens present in said liquid and then removing said complexes from said liquid to yield a partially purified liquid substantially free of antigens specific to said one or more monoclonal antibodies, (c) immunizing an animal with said partially purified liquid, (d) fusing spleen cells from said immunized animal to myeloma cells to form hybridomas capable of producing monoclonal antibodies, (e) culturing said hybridomas to produce said monoclonal antibodies, (f) isolating one or more of said monoclonal antibodies, (g) repeating steps (b) through (f) in sequence, at least once, using in step (b) at least one monoclonal antibody of step (f) in the previous sequence, and (h) terminating said method when, or before, said liquid contains one antigen.