US4427646A
Expired / Lapsed
US
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Use of radiolabeled peptide derived from crosslinked fibrin to locate thrombi in vivo
Assignee: Temple University
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Filing: 1981-04-02
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Granted: 1984-01-24
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Expires: 2001-04-02
Abstract
Abstract A method of locating thrombi in vivo comprising the steps of administering a radiolabeled peptide selected from the group consisting of Fragment E1 isolated from cross-linked fibrin, Fragment E2 isolated from crosslinked fibrin, and peptides having an amino acid sequence intermediate between Fragments E1 and E2 derived from crosslinked fibrin by plasmin digestion to a human or animal wherein the peptide is selectively incorporated or bound by forming a pre-formed thrombi and externally detecting the radiation emitted by the radiolabeled peptide. A composition containing the peptide used for locating thrombi is also disclosed.
Inventors
- 👤 Stephanie A. Olexa
- 👤 Linda C. Knight
- 👤 Andrei Z. Budzynski
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 02:19
CPC Classifications
A; A61; A61K; A61K31/00; A61K31/70; A61K31/715; A61K38/00; A61K38/16; A61K38/17; A61K38/39; A61K51/00; A61K51/02; A61K51/04; A61K51/08; C; C07; C07K; C07K14/00; C07K14/435; C07K14/78; A61K2123/00
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
63 words
A method of locating a thrombus, comprising the steps of: administering a radiolabeled peptide selected from the group consisting of Fragment E 1 isolated from cross-linked fibrin, Fragment E 2 isolated from cross-linked fibrin, and peptides having an amino acid sequence intermediate between Fragments E 1 and E 2 to a human or animal, and externally detecting radiation emitted by said radiolabeled peptide.
Claim 2
Independent Claim
63 words
A method of locating a thrombus, comprising the steps of: administering a radiolabeled peptide selected from the group consisting of Fragment E 1 isolated from cross-linked fibrin, Fragment E 2 isolated from cross-linked fibrin, and peptides having an amino acid sequence intermediate between Fragments E 1 and E 2 to a human or animal, and externally detecting radiation emitted by said radiolabeled peptide.
Claim 3
Dependent Claim
19 words
The method of claim 1, wherein said peptide is labeled with a radioactive isotope of iodine, technetium, or indium.
Claim 4
Dependent Claim
16 words
The method of claim 2, wherein said peptide is radiolabeled with 125 I or 99m Tc.
Claim 5
Dependent Claim
12 words
The method of claim 1, wherein said administering is by intravenous injection.
Claim 6
Dependent Claim
17 words
The method of claim 4, wherein said administering is in the presence of a pharmaceutically acceptable carrier.
Claim 7
Dependent Claim
18 words
The method of claim 1, wherein said detecting is by scintillation scanning, gamma camera imaging or hand-held probe.
Claim 8
Dependent Claim
15 words
The method of claim 1, wherein radiolabeled Fragment E 1 is administered to a human.
Claim 9
Dependent Claim
68 words
The method of claim 1, wherein said Fragment E 1 has an amino acid sequence identical to that of human fibrinogen amino acids α, 17-78; α, 17-78; β, 15-122; β, 15-122; γ, 1-62; and γ, 1-62 and wherein said Fragment E 2 has an amino acid sequence identical to that of human fibrinogen amino acids α, 17-78; α, 17-78; β, 15-121; β, 54-121; γ, 1-62; and γ, 1-62.
Claim 10
Dependent Claim
67 words
A composition used for locating a thrombus, comprising: radiolabeled peptide selected from the group consisting of Fragment E 1 isolated from cross-linked fibrin, Fragment E 2 isolated from cross-linked fibrin, and peptides having an amino acid sequence intermediate between Fragments E 1 and E 2 in an amount sufficient to enable external detection and location of said thrombus, and a pharmaceutically acceptable carrier suitable for intravenous injection.
Claim 11
Dependent Claim
67 words
A composition used for locating a thrombus, comprising: radiolabeled peptide selected from the group consisting of Fragment E 1 isolated from cross-linked fibrin, Fragment E 2 isolated from cross-linked fibrin, and peptides having an amino acid sequence intermediate between Fragments E 1 and E 2 in an amount sufficient to enable external detection and location of said thrombus, and a pharmaceutically acceptable carrier suitable for intravenous injection.
Claim 12
Dependent Claim
19 words
The composition of claim 9, wherein said peptide is labeled with a radioactive isotope of iodine, technetium, or indium.
Claim 13
Dependent Claim
22 words
The composition of claim 9, wherein said peptide is labeled with 111 In, 99m Tc, 125 I, 131 I or 123 I.
Claim 14
Dependent Claim
21 words
The composition of claim 9, wherein said carrier is a non-toxic isotonic aqueous solution of a salt or a non-ionic compound.
Claim 15
Dependent Claim
18 words
The composition of claim 12, wherein said carrier is a non-toxic isotonic aqueous solution of NaCl or glucose.
Claim 16
Dependent Claim
11 words
The composition of claim 9 wherein said composition further comprises heparin.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-01-23 | 1987-07-25 | 1988-01-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-01-23 | 1991-07-25 | 1992-01-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-01-23 | 1995-07-25 | 1996-01-24 | $7700.00 | Window Closed / Lapsed |