Analytical element

Claim 2

An analytical element for determining the presence of a component in a liquid sample comprising: (a) a light-transmissive and liquid-impermeable support; (b) at least one reagent layer containing at least one reagent reactive with said component and containing a hydrophilic colloid; and (c) at least one hydrophobic developing layer having a fibrous structure and being positioned on the side of said reagent layer opposite to said support whereby the component in said liquid sample permeates through said developing layer toward said reagent layer, said developing layer formed by coating said reagent layer with a homogeneous dispersion comprising a hydrophobic organic macromolecular polymer having reactive groups and a fibrous material.

Claim 3

The analytical element of claim 1, wherein said dispersion contains said hydrophobic organic macromolecular polymer having reactive groups in an amount of 0.005 to 50% by weight based on said fibrous material.

Claim 4

The analytical element of claim 1, wherein said reactive groups of the hydrophobic organic macromolecular polymer are selected from the group consisting of epoxy, aziridyl, formyl, hydroxymethyl, isocyanate, thiol and carbamoyl groups.

Claim 5

The analytical element of claim 1, wherein said fibrous material for the developing layer is selected from the group consisting of natural celluloses, natural cellulose derivatives, polyethylenes, polypropylenes and polyamides.

Claim 6

The analytical element of claim 1, wherein said developing layer has a thickness of 30 to 600 microns.

Claim 7

The analytical element of claim 1, wherein said dispersion contains at least one surfactant.

Claim 8

The analytical element of claim 6, wherein said dispersion contains said surfactant in an amount of 0.005 to 30% by weight based on said fibrous material.

Claim 9

The analytical element of claim 1, wherein said support has a thickness of about 50 to 250 microns.

Claim 10

The analytical element of claim 8, wherein said support comprises a compound selected from cellulose acetates, polyethyleneterephthalates, polycarbonates and polystyrenes.

Claim 11

The analytical element of claim 1, wherein said hydrophilic colloid is selected from the group consisting of gelatins, gelatin derivatives, polyvinyl alcohols and polyvinyl pyrrolidones.

Claim 12

The analytical element of claim 10, wherein said hydrophilic colloid is gelatin.

Claim 13

The analytical element of claim 10, wherein said hydrophilic colloid has a film thickness of at least about 5 microns.

Claim 14

The analytical element of claim 1, wherein said hydrophobic organic macromolecular polymer consists essentially of a copolymer having at least one monomer having reactive groups and at least one monomer selected from the group consisting of: ##STR2## wherein each of R 1 and R 2 , which can be the same or different, is a non-interfering substituent selected from a hydrogen atom, a halogen atom, and a substituted or unsubstituted amino-free alkyl or aryl group having 1 to 10 carbon atoms and R 3 is selected from a hydrogen atom, a halogen atom, or a substituted or unsubstituted, amino-free aliphatic or aromatic group having 1 to 10 carbon atoms; (II) CHR.sup.6 ═CR.sup.4 --COOR.sup.5 wherein R 6 has the same meaning as R 1 in formula (I), R 4 is selected from the group consisting of a hydrogen atom and a methyl group and R 5 is selected from the group consisting of an aryl, an alkyl, an alkaryl and aralkyl group, each having 1 to 10 carbon atoms; (III) polymerizable unsaturated nitrile monomers; and (IV) interparticle crosslinking monomers having two addition-polymerizable groups.

Claim 15

The analytical element of claim 13, wherein the copolymer is comprised of 0.1 to 30% by weight of the monomer having reactive groups, 0 to 99.5% by weight of the monomer (I), 0 to 99.5% by weight of the monomer (II), 0 to 99.5% by weight of the monomer (III) and 0 to 10% by weight of the monomer (IV).

Claim 16

The analytical element of claim 13, wherein the monomer having reactive groups is present in an amount of 0.1 to 30% by weight based on said hydrophobic organic macromolecular polymer.

Claim 17

The analytical element of claim 15, wherein the monomer having reactive groups is present in an amount of 0.5 to 20% by weight based on said hydrophobic organic macromolecular polymer.

Claim 18

An analytical element for determining the presence of a component in a liquid sample comprising: (a) a light-transmissive and liquid-impermeable support selected from cellulose acetates, polyethyleneterephthalates, polycarbonates and polystyrenes; (b) at least one reagent layer containing at least one reagent reactive with said component and containing a hydrophilic colloid selected from the group consisting of gelatins, gelatin derivatives, polyvinyl alcohols and polyvinyl pyrrolidones, said hydrophilic colloid having a film thickness of at least about 5 microns; and (c) at least one hydrophobic developing layer having a fibrous structure and being positioned on the side of said reagent layer opposite to said support whereby the component in said liquid sample permeates through said developing layer toward said reagent layer, said developing layer formed by coating said reagent layer with a homogeneous dispersion comprising a hydrophobic organic macromolecular polymer having reactive groups and a fibrous material, said hydrophobic organic macromolecular polymer consisting essentially of a copolymer having at least one monomer having reactive groups and at least one monomer selected from the group consisting of: ##STR3## wherein each of R 1 and R 2 , which can be the same or different, is a non-interfering substituent selected from a hydrogen atom, a halogen atom, and a substituted or unsubstituted amino-free alkyl or aryl group having 1 to 10 carbon atoms and R 3 is selected from a hydrogen atom, a halogen atom, or a substituted or unsubstituted, amino-free aliphatic or aromatic group having 1 to 10 carbon atoms; (II) CHR.sup.6 ═CR.sup.4 -COOR.sup.5 wherein R 6 has the same meaning as R 1 in formula (I), R 4 is selected from the group consisting of a hydrogen atom and a methyl group and R 5 is selected from the group consisting of an aryl, an alkyl, an alkaryl and aralkyl group, each having 1 to 10 carbon atoms; (III) polymerizable unsaturated nitrile monomers; and (IV) interparticle crosslinking monomers having two addition-polymerizable groups, wherein the monomer having reactive groups is present in an amount of 0.1 to 30% by weight based on said hydrophobic organic macromolecular polymer.

Claim 19

An analytical element for determining the presence of a component in a liquid sample comprising: (a) a light-transmissive and liquid-impermeable support selected from cellulose acetates, polyethyleneterephthalates, polycarbonates and polystyrenes; (b) at least one reagent layer containing at least one reagent reactive with said component and containing a hydrophilic colloid selected from the group consisting of gelatins, gelatin derivatives, polyvinyl alcohols and polyvinyl pyrrolidones, said hydrophilic colloid having a film thickness of at least about 5 microns; and (c) at least one hydrophobic developing layer having a fibrous structure and being positioned on the side of said reagent layer opposite to said support whereby the component in said liquid sample permeates through said developing layer toward said reagent layer, said developing layer formed by coating said reagent layer with a homogeneous dispersion comprising a hydrophobic organic macromolecular polymer having reactive groups and a fibrous material, said hydrophobic organic macromolecular polymer consisting essentially of a copolymer having at least one monomer having reactive groups and at least one monomer selected from the group consisting of: ##STR3## wherein each of R 1 and R 2 , which can be the same or different, is a non-interfering substituent selected from a hydrogen atom, a halogen atom, and a substituted or unsubstituted amino-free alkyl or aryl group having 1 to 10 carbon atoms and R 3 is selected from a hydrogen atom, a halogen atom, or a substituted or unsubstituted, amino-free aliphatic or aromatic group having 1 to 10 carbon atoms; (II) CHR.sup.6 ═CR.sup.4 -COOR.sup.5 wherein R 6 has the same meaning as R 1 in formula (I), R 4 is selected from the group consisting of a hydrogen atom and a methyl group and R 5 is selected from the group consisting of an aryl, an alkyl, an alkaryl and aralkyl group, each having 1 to 10 carbon atoms; (III) polymerizable unsaturated nitrile monomers; and (IV) interparticle crosslinking monomers having two addition-polymerizable groups, wherein the monomer having reactive groups is present in an amount of 0.1 to 30% by weight based on said hydrophobic organic macromolecular polymer.