Patent US4419509A successfully refreshed from Google Patents.
US4419509A
Expired / Lapsed
US
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Process for de-cyanoethylating blocked nucleotides
Assignee: Eli Lilly and Co
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Filing: 1981-08-24
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Granted: 1983-12-06
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Expires: 2001-08-24
Abstract
Abstract A β-cyanoethyl blocking group is selectively removed from the phosphate moiety of a nucleotide or polynucleotide by treatment with a primary amine having from 3 to 5 carbon atoms.
Inventors
- 👤 Hansen M. Hsiung
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 10:02
CPC Classifications
C; C07; C07H; C07H21/00
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
36 words
A process for selectively removing a β-cyanoethyl blocking group from the phosphate moiety of a nucleotide or polynucleotide, which comprises treating said nucleotide or polynucleotide with a primary amine having from 3 to 5 carbon atoms.
Claim 2
Dependent Claim
36 words
A process for selectively removing a β-cyanoethyl blocking group from the phosphate moiety of a nucleotide or polynucleotide, which comprises treating said nucleotide or polynucleotide with a primary amine having from 3 to 5 carbon atoms.
Claim 3
Dependent Claim
27 words
Process of claim 1, in which the removal of the β-cyanoethyl group is carried out at a temperature of from about 4° C. to about 30° C.
Claim 4
Dependent Claim
18 words
Process of claim 2, in which the removal of the β-cyanoethyl group is carried out at room temperature.
Claim 5
Dependent Claim
23 words
Process of claim 2, in which the removal of the β-cyanoethyl group is carried out in the presence of an inert organic solvent.
Claim 6
Dependent Claim
20 words
Process of claim 4, in which the removal of the β-cyanoethyl group is carried out in the presence of pyridine.
Claim 7
Dependent Claim
11 words
Process of claim 4, in which the primary amine is t-butylamine.
Claim 8
Dependent Claim
11 words
Process of claim 4, in which the primary amine is sec-butylamine.
Claim 9
Dependent Claim
11 words
Process of claim 4, in which the primary amine is n-propylamine.
References Cited (Backward Citations)
US3284440A
Cited
US4310662A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-12-05 | 1987-06-06 | 1987-12-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-12-05 | 1991-06-06 | 1991-12-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-12-05 | 1995-06-06 | 1995-12-06 | $7700.00 | Window Closed / Lapsed |