Patent US4418149A successfully refreshed from Google Patents.
US4418149A
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US
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Fused hybrid gene
Assignee: Harvard University
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Filing: 1982-02-05
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Granted: 1983-11-29
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Expires: 2002-02-05
Abstract
Abstract This invention is a process to produce specific proteins coded for by eukaryotic (or prokaryotic) DNA in bacteria. The invention, which uses recombinant DNA techniques, produces proteins in their natural, functional state unencumbered by extraneous peptides.
Inventors
- 👤 Mark Ptashne
- 👤 Gail D. Lauer
- 👤 Thomas M. Roberts
- 👤 Keith C. Backman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 16:21
CPC Classifications
C; C12; C12P; C12P21/00; C12N; C12N15/00
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
77 words
A fused hybrid gene capable of expressing native unfused prokaryotic or eukaryotic protein consisting essentially of (1) a portable promoter including a portion of a bacterial gene having a Shine-Dalgarno sequence and a transcription initiation site recognized by RNA polymerase and containing no protein translational start site, and (2) fused thereto a gene for a native unfused prokaryotic or eukaryotic protein including its translational start site, said portable promoter being located upstream from said translational start site.
Claim 2
Dependent Claim
77 words
A fused hybrid gene capable of expressing native unfused prokaryotic or eukaryotic protein consisting essentially of (1) a portable promoter including a portion of a bacterial gene having a Shine-Dalgarno sequence and a transcription initiation site recognized by RNA polymerase and containing no protein translational start site, and (2) fused thereto a gene for a native unfused prokaryotic or eukaryotic protein including its translational start site, said portable promoter being located upstream from said translational start site.
Claim 3
Dependent Claim
22 words
A fused gene as claimed in claim 1 in which said promoter is the product of restriction endonuclease digestion of an operon.
Claim 4
Dependent Claim
35 words
A fused gene as claimed in claim 2 in which said bacterial gene is that of E. coli and said promoter is the product of restriction endonuclease digestion of the lac operon of E. coli.
Claim 5
Dependent Claim
26 words
A fused gene as claimed in claim 1 wherein said translational start site of said gene for said prokaryotic or eukaryotic protein is the sequence ATG.
Claim 6
Dependent Claim
30 words
A fused gene as claimed in claim 1 wherein said portable promoter and said translational start site of said prokaryotic or eukaryotic gene together comprise a hybrid ribosomal binding site.
Claim 7
Dependent Claim
24 words
A fused gene as claimed in claim 1 wherein said gene for a prokaryotic or eukaryotic protein is a gene for a eukaryotic protein.
Claim 8
Dependent Claim
21 words
A bacterium containing the fused gene of claim 1, said bacterium being capable of producing said prokaryotic or said eukaryotic protein.
Claim 9
Dependent Claim
18 words
A bacterium containing the fused gene of claim 6, said bacterium being capable of producing said eukaryotic protein.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-11-28 | 1987-05-30 | 1987-11-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-11-28 | 1991-05-30 | 1991-11-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-11-28 | 1995-05-30 | 1995-11-29 | $7700.00 | Window Closed / Lapsed |