US4416784A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Filling composition for use in liquid chromatography
Assignee: Kureha Corp
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Filing: 1982-02-23
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Granted: 1983-11-22
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Expires: 2002-02-23
Abstract
Abstract Disclosed herein is a bead-like substance comprising a hydroxymethylated copolymer of styrene and divinylbenzene having a serum protein adsorbed or adhering thereonto, suitable for filling the column used in liquid chromatography in the field of analysis, particularly in clinical examinations, and a method for analytically examining a physiological specimen by liquid column chromatography.
Inventors
- 👤 Masaaki Nakao
- 👤 Fumio Kodama
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 01:46
CPC Classifications
B; B01; B01D; B01D15/00; B01D15/08; B01D15/26; B01D15/38; B01D15/3804; B01J; B01J20/00; B01J20/281; B01J20/282; B01J20/285; B01D15/10; B01D15/20; B01D15/206; B01J2220/00; B01J2220/50; B01J2220/54
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
131 words
A method for analytically examining a physiological specimen by liquid column chromatography comprising the steps of: (a) stabilizing a column comprised of bead-like hydroxymethylated copolymer of styrene and divinylbenzene by passing a phosphate buffer solution or citrate buffer solution in the range of pH 6.5 to 8.0 through the column at a temperature of 5° to 70° C.; (b) further stabilizing the column by passing an aqueous solution of serum protein containing 0.2 to 5.0% by weight of said serum protein through the column at a temperature of 5° to 70° C. wherein the serum protein is absorbed onto the column; and (c) passing the physiological specimen to be examined through the buffer and protein stabilized column at a temperature of 20° to 40° C. in order to examine the specimen.
Claim 2
Independent Claim
131 words
A method for analytically examining a physiological specimen by liquid column chromatography comprising the steps of: (a) stabilizing a column comprised of bead-like hydroxymethylated copolymer of styrene and divinylbenzene by passing a phosphate buffer solution or citrate buffer solution in the range of pH 6.5 to 8.0 through the column at a temperature of 5° to 70° C.; (b) further stabilizing the column by passing an aqueous solution of serum protein containing 0.2 to 5.0% by weight of said serum protein through the column at a temperature of 5° to 70° C. wherein the serum protein is absorbed onto the column; and (c) passing the physiological specimen to be examined through the buffer and protein stabilized column at a temperature of 20° to 40° C. in order to examine the specimen.
Claim 3
Dependent Claim
45 words
The method according to claim 1, wherein said solution of a serum protein is prepared by dissolving said serum protein in a solvent selected from the group consisting of water, a buffer solution, a buffer solution containing a salt dissolved therein, and an organic solvent.
Claim 4
Dependent Claim
16 words
The method according to claim 1, wherein said liquid column chromatography is high-speed liquid column chromatography.
Claim 5
Dependent Claim
24 words
The method according to claim 1, wherein said physiological specimen is a blood component, cerebrospinal fluid, lymph, ascites fluid, urine or other body fluid.
Claim 6
Dependent Claim
24 words
The method according to claim 1, wherein the pH of said aqueous solution of serum protein is in the range of 5 to 9.
Claim 7
Dependent Claim
24 words
The method according to claim 1, wherein the pH of said phosphate buffer or citrate buffer is in the range of 7.0 to 7.7.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-11-21 | 1987-05-23 | 1987-11-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-11-21 | 1991-05-23 | 1991-11-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-11-21 | 1995-05-23 | 1995-11-22 | $7700.00 | Window Closed / Lapsed |