Patent US4411994A successfully refreshed from Google Patents.
US4411994A
Expired / Lapsed
US
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Protein synthesis
Assignee: Biogen Inc
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Filing: 1978-06-08
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Granted: 1983-10-25
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Expires: 1998-06-08
Abstract
Abstract A plasmid or phage gene for a periplasmic or extracellular bacterial protein is cleaved, a double-stranded DNA sequence coding for a selected protein or portion thereof from a eukaryotic cell such as insulin is inserted in that cleaved gene by recombinant DNA techniques and used to transform a bacterium, and the excreted selected protein is collected.
Inventors
- 👤 Walter Gilbert
- 👤 Stephanie A. Broome
- 👤 Lydia J. Villa-Komaroff
- 👤 Argiris A. Efstratiadis
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 22:40
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/62; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/66; C12N9/00; C12N9/14; C12N9/78; C12N9/86; C07K2319/00; C07K2319/01; C07K2319/02; C07K2319/034; C07K2319/70; C07K2319/74; C07K2319/75; Y; Y10; Y10S; Y10S930/00; Y10S930/01; Y10S930/30
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
95 words
The method of making within a bacterial host and excreting through the membrane of the host cell a selected protein or polypeptide which comprises cleaving a bacterial gene for an extracellular or periplasmic carrier protein, within a cloning vehicle selected from the group comprising plasmids or phage genes, forming a hybrid gene by inserting into that cleavage site a non-bacterial DNA fragment which codes for the selected protein or polypeptide, transforming said bacterial host with the hybrid gene, and culturing the transformed bacteria to express the hybrid gene and excrete the selected protein or polypeptide.
Claim 2
Independent Claim
95 words
The method of making within a bacterial host and excreting through the membrane of the host cell a selected protein or polypeptide which comprises cleaving a bacterial gene for an extracellular or periplasmic carrier protein, within a cloning vehicle selected from the group comprising plasmids or phage genes, forming a hybrid gene by inserting into that cleavage site a non-bacterial DNA fragment which codes for the selected protein or polypeptide, transforming said bacterial host with the hybrid gene, and culturing the transformed bacteria to express the hybrid gene and excrete the selected protein or polypeptide.
Claim 3
Dependent Claim
32 words
The method as claimed in claim 1 in which the selected protein is a eukaryotic cell protein which contains a hydrophobic leader which is normally cleaved during excretion from the eukaryotic cell.
Claim 4
Independent Claim
18 words
The method as claimed in claims 1 or 2 in which the carrier protein is E. coli penicillinase.
Claim 5
Independent Claim
19 words
The method as claimed in any of claims 1 to 3 inclusive in which the selected protein is insulin.
Claim 6
Dependent Claim
27 words
The method as claimed in claim 1 in which the carrier protein is E. coli penicillinase and the bacterial gene is cleaved at the Pst restriction site.
References Cited (Backward Citations)
GB1521032A
Cited
US4082613A
Cited
GB1516458A
Cited
EP0001929A2
Cited
GB2031434B
Cited
GB2033905B
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-10-24 | 1987-04-25 | 1987-10-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-10-24 | 1991-04-25 | 1991-10-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-10-24 | 1995-04-25 | 1995-10-25 | $7700.00 | Window Closed / Lapsed |