US4411915A
Expired / Lapsed
US
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Heme-iron-enriched amino acid and a process for the preparation of heme-iron-enriched amino acid
Assignee: Japan Tobacco Inc
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Filing: 1981-04-18
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Granted: 1983-10-25
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Expires: 2001-04-18
Abstract
Abstract There is disclosed heme-iron-enriched amino acid preparation consisting of aggregates of heme-protein fragments with heme bonded to histidine groups present in the fragment and a method for preparing this amino acid preparation from heme-proteins, especially hemoglobin. The heme-protein in a manner known per se at first is hemolyzed and denaturated and subsequently split with the aid of proteolytic enzymes to the formation of one heme-iron-enriched and one fraction which does not contain heme-iron and separation of these fractions from each other. The amino acid preparation is suitable for iron enrichment of for instance foodstuffs and for use in pharmaceutical iron preparations.
Inventors
- 👤 Caj Eriksson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 23:16
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/795; C07K14/805; A; A23; A23J; A23J3/00; A23J3/30; A23J3/32; A23J3/34; A23J3/341; A23J3/345; A23L; A23L33/00; A23L33/10; A23L33/17; A23L33/18; A61; A61K; A61K38/00; A61K38/01; A61K38/012; A61K38/017; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
62 words
Heme-iron-enriched amino acid product prepared from heme-proteins, wherein the iron content of the preparation consists solely of the porphyrin iron of the original heme-protein which is bonded as heme to amino acid chains which are shorter than the amino acid chains in the original heme-protein, but which are aggregated into units having larger dimensions than the separate, short, heme-iron-containing amino acid chains.
Claim 2
Dependent Claim
62 words
Heme-iron-enriched amino acid product prepared from heme-proteins, wherein the iron content of the preparation consists solely of the porphyrin iron of the original heme-protein which is bonded as heme to amino acid chains which are shorter than the amino acid chains in the original heme-protein, but which are aggregated into units having larger dimensions than the separate, short, heme-iron-containing amino acid chains.
Claim 3
Dependent Claim
42 words
Amino acid preparation according to claim 1, which is comprised by a constant ratio between heme and iron and by a porphyrin heme-iron content of at least 0.5% and at the most 5%, calculated as the iron which is bonded to porphyrin.
Claim 4
Dependent Claim
58 words
A process for the preparation of heme-iron-enriched amino acid preparations from heme-proteins, wherein the heme-protein is first hemolyzed and denaturated and subsequently split up with the aid of proteolytic enzymes and forming one heme-iron-enriched fraction and one fraction which does not contain heme-iron and recovering the heme-iron-enriched fraction by separation and stabilizing it by heat treatment and dewatering.
Claim 5
Dependent Claim
58 words
A process for the preparation of heme-iron-enriched amino acid preparations from heme-proteins, wherein the heme-protein is first hemolyzed and denaturated and subsequently split up with the aid of proteolytic enzymes and forming one heme-iron-enriched fraction and one fraction which does not contain heme-iron and recovering the heme-iron-enriched fraction by separation and stabilizing it by heat treatment and dewatering.
Claim 6
Dependent Claim
27 words
A process according to claim 3, wherein the enzyme is obtained from preparations containing proteolytic enzymes selected from the group consisting of animals, plants, microorganisms or fungi.
Claim 7
Dependent Claim
19 words
A process according to claim 3 or 4, wherein the hydrolysis is conducted at the pH range of 4-11.
Claim 8
Dependent Claim
17 words
A process according to claim 5 wherein the hydrolysis is performed at a temperature of 10°-80° C.
Claim 9
Dependent Claim
28 words
A process according to claim 6, wherein the heme-iron-enriched fraction is separated from the amino acid-enriched fraction by precipitation means at a pH of 4-5 or by ultrafiltration.
Claim 10
Dependent Claim
16 words
A process according to claim 7, which comprises conducting the ultrafiltration at a pH of 5-9.
Claim 11
Dependent Claim
38 words
A process according to claim 7 wherein the separation is performed with the use of a membrane which has a separation degree of 10000-50,000 dalton or with a combination of membranes with a separation degree of 1000-50,000 dalton.
Claim 12
Dependent Claim
24 words
A process according to claim 7 which comprises separating the heme-iron-enriched fraction from the amino acid-enriched fraction by a combination of ultrafiltration and precipitation.
Claim 13
Dependent Claim
24 words
A process according to claim 9 which comprises separating the heme-iron-enriched fraction from the amino acid-enriched fraction by a combination of ultrafiltration and precipitation.
Claim 14
Dependent Claim
38 words
A process according to claim 8, wherein the separation is performed with the use of a membrane which has a separation degree of 1000-50,000 dalton or with a combination of membranes with a separation degree of 1000-50,000 dalton.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-10-24 | 1987-04-25 | 1987-10-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-10-24 | 1991-04-25 | 1991-10-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-10-24 | 1995-04-25 | 1995-10-25 | $7700.00 | Window Closed / Lapsed |