US4262022A
Expired / Lapsed
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Method for preparing a food material from blood
Assignee: Danish Technological Institute
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Filing: 1979-02-05
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Granted: 1981-04-14
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Expires: 1999-02-05
Abstract
Abstract A material based on blood, in which the red corpuscle fraction has been partially proteolytically hydrolyzed and thereby decoloured, to be used as an additive to food products, especially meat, due to the nutritional and functional properties of this material.
Inventors
- 👤 Vilhelm Hald-Christensen
- 👤 Jens L. Adler-Nissen
- 👤 Hans S. Olsen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 10:27
CPC Classifications
A; A23; A23J; A23J3/00; A23J3/30; A23J3/32; A23J3/34; A23J3/341; A23J3/345; A23J3/04; A23J3/12
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
74 words
A method for producing a decolorized edible material from blood which comprises: (a) haemolyzing a red blood corpuscle containing blood fraction; (b) then partially hydrolyzing the haemolyzed blood fraction enzymatically with the proteinase of B. licheniformis to a degree of hydrolysis of at least 10, forming thereby a hydrolysate constituted of a decolorized supernatant containing partially hydrolyzed edible protein dissolved therein and a sludge; and, (c) thereafter deactivating the proteinase and recovering the supernatant.
Claim 2
Independent Claim
74 words
A method for producing a decolorized edible material from blood which comprises: (a) haemolyzing a red blood corpuscle containing blood fraction; (b) then partially hydrolyzing the haemolyzed blood fraction enzymatically with the proteinase of B. licheniformis to a degree of hydrolysis of at least 10, forming thereby a hydrolysate constituted of a decolorized supernatant containing partially hydrolyzed edible protein dissolved therein and a sludge; and, (c) thereafter deactivating the proteinase and recovering the supernatant.
Claim 3
Dependent Claim
23 words
The method of claim 1 wherein the method commences by separating whole blood into a plasma fraction and a red blood corpuscle fraction.
Claim 4
Dependent Claim
23 words
The method according to claim 2 wherein the supernatant from the treated red blood corpuscle fraction is thereafter combined with the plasma fraction.
Claim 5
Dependent Claim
28 words
Method according to claim 1 wherein the haemolysis is performed by addition of water with a volume of 2-5 times the volume of the red blood corpuscle fraction.
Claim 6
Dependent Claim
18 words
Method according to claim 1, wherein the hydrolysis is carried out to a DH between 14 and 22.
Claim 7
Dependent Claim
29 words
Method according to claim 1, wherein the hydrolysis is performed at pH 7-10 and with an enzyme activity of between 6 and 60 Anson units per kg of protein.
Claim 8
Dependent Claim
20 words
Method according to claim 6, wherein the pH is kept approximately constant during the hydrolysis by addition of a base.
Claim 9
Dependent Claim
35 words
A method according to claim 1, wherein the hydrolyzing mixture is maintained at a constant temperature level during the first half of the hydrolysis and thereafter is raised during the last half of the hydrolysis.
Claim 10
Dependent Claim
40 words
Method according to claim 8, wherein the temperature of the hydrolyzing mixture is around 55° C. during the first half of the hydrolysis, whereafter the temperature gradually is raised to about 65° C. during the last half of the hydrolysis.
Claim 11
Dependent Claim
23 words
The method according to claim 1 wherein the inactivation of the proteolytic enzyme is carried out by addition of acid to the hydrolysate.
Claim 12
Dependent Claim
20 words
A method according to claim 1, wherein the inactivation of the proteolytic enzyme is carried out by heating the hydrolyzate.
Claim 13
Dependent Claim
44 words
A method according to claim 1 which further comprises intimately mixing the supernatant with an amount of activated carbon of between 8 and 33% of the weight of the protein present to further treat said supernatant and subsequently removing the carbon from the supernatant.
Claim 14
Dependent Claim
19 words
Method according to claim 12, wherein the carbon treatment is performed at a pH value between 2.5 and 4.5.
Claim 15
Dependent Claim
15 words
A material for addition to food products, prepared by the process according to claim 1.
Claim 16
Dependent Claim
31 words
The process of claim 6 wherein the supernatant recovery process comprises adjusting the pH of the hydrolysate to the range of pH 4-5, and thereafter separating the sludge from the supernatant.
Claim 17
Independent Claim
92 words
A method for producing a decolorized edible material from blood which comprises: (a) haemolyzing a red blood corpuscle containing blood fraction; (b) then hydrolyzing the haemolyzed blood fraction to a degree of hydrolysis in the range of 14-22 at pH 7-10 with the proteinase of B. licheniformis, forming thereby a hydrolysate constituted of a decolorized supernatant containing partially hydrolyzed edible protein dissolved therein and a sludge; (c) thereafter deactivating the proteinase; (d) followed by subjecting the hydrolysate to a pH 4-5, and then separating the sludge from supernatant to recover the supernatant.
Claim 18
Independent Claim
92 words
A method for producing a decolorized edible material from blood which comprises: (a) haemolyzing a red blood corpuscle containing blood fraction; (b) then hydrolyzing the haemolyzed blood fraction to a degree of hydrolysis in the range of 14-22 at pH 7-10 with the proteinase of B. licheniformis, forming thereby a hydrolysate constituted of a decolorized supernatant containing partially hydrolyzed edible protein dissolved therein and a sludge; (c) thereafter deactivating the proteinase; (d) followed by subjecting the hydrolysate to a pH 4-5, and then separating the sludge from supernatant to recover the supernatant.
Claim 19
Dependent Claim
16 words
The method of claim 16 wherein the proteinase is deactivated by adding acid to the hydrolysate.
Claim 20
Dependent Claim
22 words
The method of claim 16 wherein hydrolysis is carried out with a proteinase concentration of 6-60 Anson units per kg of protein.
Claim 21
Dependent Claim
27 words
The method of claim 16 wherein the supernatant is then further treated contacting the supernatant at pH 2.5-3.5 with activated carbon, followed by removing the carbon therefrom.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-04-13 | 1984-10-13 | 1985-04-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-04-13 | 1988-10-13 | 1989-04-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-04-13 | 1992-10-13 | 1993-04-14 | $7700.00 | Window Closed / Lapsed |