Patent US4409329A successfully refreshed from Google Patents.
US4409329A
Expired / Lapsed
US
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Saccharification method
Assignee: Chevron USA Inc
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Filing: 1982-07-15
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Granted: 1983-10-11
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Expires: 2002-07-15
Abstract
Abstract Enzymic conversion of cellulose under mechanical shear rate of from 50,000 to 200,000 feet/minute/foot as imparted to the cellulose by a series of rotors and stators mounted in the reaction vessel improves yield of simple sugars.
Inventors
- 👤 Richard S. Silver
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 18:47
CPC Classifications
C; C13; C13K; C13K1/00; C13K1/02; C12; C12P; C12P19/00; C12P19/14; C12P7/00; C12P7/02; C12P7/04; C12P7/06; C12P7/08; C12P7/10; Y; Y02; Y02E; Y02E50/00; Y02E50/10
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
103 words
A method for the hydrolysis of cellulose to simple sugars which comprises: contacting in a reaction zone an aqueous slurry comprising from three to 20 weight percent of a solid cellulose containing charge stock with a cellulase enzyme complex wherein the concentration of said enzyme complex is greater than 0.1 units per milliliter of said slurry and wherein said contacting occurs in the presence of a mechanically produced shear rate of from 50,000 to 200,000 feet/minute/foot substantially throughout said reaction zone and wherein the cellulose content of said solid cellulose-containing charge stock is from 30 to 80 weight percent of said charge stock.
Claim 2
Independent Claim
103 words
A method for the hydrolysis of cellulose to simple sugars which comprises: contacting in a reaction zone an aqueous slurry comprising from three to 20 weight percent of a solid cellulose containing charge stock with a cellulase enzyme complex wherein the concentration of said enzyme complex is greater than 0.1 units per milliliter of said slurry and wherein said contacting occurs in the presence of a mechanically produced shear rate of from 50,000 to 200,000 feet/minute/foot substantially throughout said reaction zone and wherein the cellulose content of said solid cellulose-containing charge stock is from 30 to 80 weight percent of said charge stock.
Claim 3
Dependent Claim
35 words
A method in accordance with claim 1 wherein said shear rate is generated by a series of spaced apart rotors and stators mounted in said reaction zone containing the enzyme complex in said aqueous slurry.
Claim 4
Dependent Claim
25 words
A method in accordance with claim 2 wherein said solid cellulose-containing charge stock has a mean particle size from 0.01 to 1 inch in diameter.
Claim 5
Dependent Claim
21 words
A method in accordance with claim 2 wherein the rotors and stators comprise less than 50 percent of the reactor volume.
Claim 6
Dependent Claim
21 words
A method in accordance with claim 2 wherein the spacing between the rotors and stators is from 0.1 to 50 mm.
Claim 7
Dependent Claim
33 words
A method in accordance with claim 5 wherein said solid cellulose-containing charge stock has a mean particle size such that substantially no particles are larger than the spacing between rotor and stator blades.
Claim 8
Dependent Claim
30 words
A process in accordance with claim 1 wherein said process is operated in a conjoint presence of a fermentation microorganism for the simultaneous hydrolysis and fermentation of cellulose to ethanol.
Claim 9
Dependent Claim
38 words
A process in accordance with claim 6 wherein said fermentation microorganism is selected from the group consisting of yeasts such as Saccharomyces cerevisiae or Candida brassicae or bacteria such as Zymomonas mobilis or molds such as Rhizopus javanicus.
Claim 10
Dependent Claim
25 words
A process in accordance with claim 7 wherein the concentration of said enzyme complex is from 0.1 to 5 units per milliliter of said slurry.
Claim 11
Dependent Claim
28 words
A process in accordance with claim 8 wherein the concentration of said fermentation microorganism is such that the concentration of produced sugar is at all times substantially zero.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-10-10 | 1987-04-11 | 1987-10-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-10-10 | 1991-04-11 | 1991-10-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-10-10 | 1995-04-11 | 1995-10-11 | $7700.00 | Window Closed / Lapsed |