Heat-gelling and foam-stabilizing enzymatically modified vegetable isolates

Claim 2

An enzymatically modified vegetable protein composition possessing improved heat-gelling properties, prepared from 7S glycoproteins which contains (on a total protein weight basis), enzymatically modified 7S glycoprotein as the major protein component of the composition, said composition comprising enzymatically modified 7S protein in which the glycoprotein aggregates have an average molecular weight within the 200,000 to 300,000 molecular weight range, said glycoprotein aggregates consist essentially of a plurality of glycoprotein subunits of an average molecular weight of less than the glycoprotein subunit precursor source material for said enzymatically modified protein composition and the average electrophoretic migration value for the enzymatically modified subunits is less than that of the 7S glycoprotein subunits precursor source material for said composition, said composition being further characterized by forming a solid, opaque gel when an aqueous solution adjusted to 0.1 M sodium chloride concentration and pH 5.75 and containing 15 protein grams of said composition for each 100 ml of water is heated at 85° C. for 15 minutes.

Claim 3

The composition according to claim 1 wherein the glycoprotein source material consists essentially of 7S glycoproteins of soy proteins.

Claim 4

The composition according to claim 2 wherein the aggregate consists essentially of six glycoprotein subunits of an average molecular weight less than 48,000.

Claim 5

The composition according to claim 2 wherein the enzymatically modified composition comprises an acid proteinase modified 7S glycoprotein.

Claim 6

The composition according to claim 2 wherein the electrophoretic migrating value of the enzymatically modified subunit has a value of about 25% to about 50% of the migrating value for the α' and α precursor subunits.

Claim 7

The composition according to claim 3 wherein the aqueous solution thereof is characterized as heat-setting into a white, heat-set gel of a penetrometer value of less than 5 when heated at 85° C. for 15 minutes.

Claim 8

The composition according to claim 6 wherein the gel obtained from the glycoprotein aggregate composition is characterized as having a penetrometer value of less than 3 mm.

Claim 9

The composition according to claim 6 wherein the aqueous solution containing the glycoprotein aggregate composition is characterized as heat-setting into a heat-set gel within five minutes.

Claim 10

The composition according to claim 3 wherein the glycoprotein aggregate composition is characteized as having an average molecular weight of about 265,000 and the subunits have an average molecular weight of about 47,000.

Claim 11

The composition according to claim 9 wherein the composition consists essentially of pepsin modified glycoprotein aggregate and the electrophoretic migrating value for the pepsin modified subunits range from about 35% to 45% of the migrating value for the α' and α subunit precursor of said aggregate composition.

Claim 12

The composition according to claim 3 wherein the glycoprotein aggregate composition is characterized as having an average molecular weight within the 250,000 to 280,000 M.W. range, and heat-setting into a white, opaque, gel when the aqueous solution thereof is heated at 85° C. for 5 minutes.

Claim 15

The method according to claim 12 wherein the source material consists essentially of soy protein.

Claim 16

The method according to claim 12 wherein the soy protein comprises an enriched 7S fraction containing (on a weight basis) at least 70% by weight of the enzymatically modified 7S glycoprotein.

Claim 17

The method according to claim 14 wherein the 7S glycoprotein is hydrolyzed with an acid proteinase for a period of time sufficient and under hydrolyzing conditions to provide a glycoprotein aggregate of an average molecular weight within the 250,000 to 280,000 M.W. range and the glycoprotein aggregate consisting essentially of six subunits in which the subunit average molecular weight is greater than 40,000 but less than 50,000.

Claim 18

The method according to claim 15 wherein the 7S glycoprotein is hydrolyzed with an acid proteinase.

Claim 19

The method according to claim 14 wherein the 7S glycoprotein is hydrolyzed with an effective amount of a proteinase under conditions sufficient to provide a glycoprotein aggregate composition characterized as having an average molecular weight within the 250,000 to 280,000 range and the aggregate composition consists essentially of six glycoprotein subunits.

Claim 20

The method according to claim 17 wherein the hydrolysis of the 7S glycoprotein provides an aggregate composition which upon dissolution in water containing 0.1 M sodium chloride at pH 5.75 and 15% aggregate composition concentration (protein dry solids weight/water volume basis) and heating at 85° C. for 5 minutes will provide a white, solid, opaque heat-set gel.

Claim 21

The method according to claim 17 wherein the hydrolysis provides an aggregate composition characterized as having a migrating value ranging from about 25% to about 60% of the migrating value for the α' and α subunit precursors of said aggregate composition.

Claim 22

The method according to claim 17 wherein the aggregate composition is characterized as having an average molecular weight of about 265,000 and the average subunit molecular weight is about 47,000.

Claim 23

In a method for preparing a heat-set gel from an aqueous solution containing a protein as a heat-gelling agent, the improvement which comprises replacing at least a portion of the heat-gelling protein agent with an enzymatically modified vegetable protein composition comprised of enzymatically modified 7S glycoprotein aggregates as the major vegetable protein component (total vegetable protein weight basis) of said composition, said aggregates being characterized as having an average molecular weight within the 200,000 to 300,000 molecular weight range with the aggregates consisting essentially of a plurality of glycoprotein subunits of an average molecular weight less than the glycoprotein subunits precursor source material for said enzymatically modified glycoprotein composition and the enzymatically modified subunits have an average electrophoretic migration value less than that of the non-enzymatically modified subunit precursor source material for said composition, and said composition is further characterized as forming a solid, opaque gel when an aqueous solution adjusted to 0.1 M sodium chloride concentration and pH 5.75 and containing 15 protein grams of said composition for each 100 ml of solution is heated at 85° C. for 15 minutes.

Claim 24

In a method for preparing a heat-set gel from an aqueous solution containing a protein as a heat-gelling agent, the improvement which comprises replacing at least a portion of the heat-gelling protein agent with an enzymatically modified vegetable protein composition comprised of enzymatically modified 7S glycoprotein aggregates as the major vegetable protein component (total vegetable protein weight basis) of said composition, said aggregates being characterized as having an average molecular weight within the 200,000 to 300,000 molecular weight range with the aggregates consisting essentially of a plurality of glycoprotein subunits of an average molecular weight less than the glycoprotein subunits precursor source material for said enzymatically modified glycoprotein composition and the enzymatically modified subunits have an average electrophoretic migration value less than that of the non-enzymatically modified subunit precursor source material for said composition, and said composition is further characterized as forming a solid, opaque gel when an aqueous solution adjusted to 0.1 M sodium chloride concentration and pH 5.75 and containing 15 protein grams of said composition for each 100 ml of solution is heated at 85° C. for 15 minutes.

Claim 25

The method according to claim 21 wherein the enzymatically modified glycoprotein consists essentially of enzymatically modified 7S soy protein.

Claim 26

The method according to claim 22 wherein the composition is characterized as heat-setting within 5 minutes into a solid, opaque gel when the aqueous solution is heated at 85° C.

Claim 27

The method according to claim 22 wherein the aggregate composition is characterized as having an average molecular weight of about 250,000 to about 280,000 and the glycoprotein aggregate consists essentially of six subunits in which the average molecular weight for the individual subunits of said aggregate ranges from about 40,000 to 50,000 M.W.

Claim 28

The method according to claim 24 wherein the composition is characterized as forming a solid, white, heat-set gel of a penetrometer value less than 5 mm when heated at 85° C. for 15 minutes.

Claim 29

A heat-set gel containing the aggregate composition of claim 21.

Claim 30

A heat-set gel containing the aggregate composition of claim 22.

Claim 31

A heat-set gel containing the aggregate composition of claim 23.

Claim 32

A heat-set gel containing the aggregate composition of claim 24.

Claim 33

A heat-set gel containing the aggregate composition of claim 25.

Claim 34

In a method for preparing foamed product from an aqueous foaming composition containing a hydrophilic foaming agent wherein gas is ingested into the foaming composition to provide a foamed product, the improvement which comprises incorporating an effective amount of an enzymatically modified glycoprotein 7S aggregate composition into the foaming composition to stabilize the foamed product against degasification, said 7S glycoprotein aggregate composition being characterized as comprising an enriched 7S vegetable protein isolate wherein the 7S glycoprotein aggregates have an average molecular weight within the 200,000 to 300,000 molecular weight range, the aggregates of the enzymatically modified protein consisting essentially of a plurality of glycoprotein subunits of an average molecular weight less than that of the unmodified glycoprotein subunit source material for said enzymatically modified glycoprotein composition and the enzymatically modified subunits have an average electrophoretic migration value less than that of the unmodified glycoprotein subunits, and the aggregate composition is further characterized as providing a solid, opaque gel when an aqueous solution adjusted to 0.1 M sodium chloride concentration and pH 5.75 and containing 15 protein grams of said aggregrate composition for each 100 ml of water is heated at 85° C. for 15 minutes.

Claim 35

In a method for preparing foamed product from an aqueous foaming composition containing a hydrophilic foaming agent wherein gas is ingested into the foaming composition to provide a foamed product, the improvement which comprises incorporating an effective amount of an enzymatically modified glycoprotein 7S aggregate composition into the foaming composition to stabilize the foamed product against degasification, said 7S glycoprotein aggregate composition being characterized as comprising an enriched 7S vegetable protein isolate wherein the 7S glycoprotein aggregates have an average molecular weight within the 200,000 to 300,000 molecular weight range, the aggregates of the enzymatically modified protein consisting essentially of a plurality of glycoprotein subunits of an average molecular weight less than that of the unmodified glycoprotein subunit source material for said enzymatically modified glycoprotein composition and the enzymatically modified subunits have an average electrophoretic migration value less than that of the unmodified glycoprotein subunits, and the aggregate composition is further characterized as providing a solid, opaque gel when an aqueous solution adjusted to 0.1 M sodium chloride concentration and pH 5.75 and containing 15 protein grams of said aggregrate composition for each 100 ml of water is heated at 85° C. for 15 minutes.

Claim 36

The method according to claim 31 wherein the enzymatically modified glycoprotein consists essentially of enzymatically modified 7S soy protein.

Claim 37

The method according to claim 32 wherein at least a major weight portion of the hydrophilic foaming agent of said foaming composition comprises a vegetable protein whipping agent.

Claim 38

The method according to claim 32 wherein the aggregate composition is characterized as having an average molecular weight of about 250,000 to about 280,000 and the glycoprotein aggregate consists essentially of six subunits in which the average molecular weight for the individual subunits of said aggregate ranges from about 40,000 to 50,000 M.W.

Claim 39

The method according to claim 34 wherein the glycoprotein is enzymatically modified with an acid proteinase to provide an aggregate composition which upon dissolution in 100 ml water of 0.1 M sodium chloride concentration at pH 5.75 and heating at 85° C. for 5 minutes will provide a white, solid, opaque heat-set gel.

Claim 40

The method according to claim 34 wherein the hydrophilic foaming agent consists essentially of a vegetable protein whipping agent.

Claim 41

The method according to claim 32 wherein the aggregate composition is characterized as having an average molecular weight of about 265,000 and the average subunit molecular weight is about 47,000.

Claim 42

The foamed product of claim 32.

Claim 43

The foamed product of claim 33.

Claim 44

The foamed product of claim 35.

Claim 45

The foamed product of claim 36.

Claim 46

The foamed product of claim 37.