US4131607A
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US
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Process for obtaining purified protein isolates
Assignee: Institut National de la Recherche Agronomique
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Filing: 1975-10-23
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Granted: 1978-12-26
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Expires: 1995-10-23
Abstract
Abstract The invention relates to the production of purified protein isolates. The process of the invention comprises: (1) making a suspension or a slurry of cake of a protein substance containing compounds having a mucilaginous consistency with a volume in 1. of 10 to 15 times and preferably 3 to 5 times, the weight in kg of press cake of a solution of one or more alkaline-earth metal comprising a magnesium salt and further containing Na sulphite, and adding to the suspension or slurry thus obtained a concentrated solution of an alkali in an amount sufficient to permit solubilization of the proteins, (2) separating in a known manner the protein extract from the insoluble residue, (3) acidifying the resulting clarified protein extract with an acid solution to a pH of between 4.5 and 5.5 to precipitate the proteins, and (4) separating in a known manner from the mother-liquors the protein precipitate formed, the said protein precipitate being subsequently subjected to washing. The protein isolates so obtained are light colored and have a nitrogen content of at least 16%.
Inventors
- 👤 Leon Petit
- 👤 Andre Davin
- 👤 Jacques Gueguen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 14:07
CPC Classifications
A; A23; A23J; A23J1/00; A23J1/14
Claims Analysis
Total Claims: 29
Claim 1
Independent Claim
129 words
A process for obtaining purified protein isolates from protein substances containing compounds having a mucilaginous consistency which comprises: (a) making a suspension of cake of such protein substance with a solution of a calcium salt and a magnesium salt of volume in 1. of 10 to 15 times the weight in Kg. of press cake which solution further contains sodium sulphite, (b) adding thereto a concentrated solution of alkali in amount sufficient to solubilize the proteins in said press cake, (c) separating the protein extract from the insoluble residue, (d) acidifying the protein extract obtained with an acid solution at a pH between 4.5 and 5.5 to precipitate the proteins, (e) separating the protein precipitate from the mother liquor, (f) washing the protein precipitate and recovering the protein isolate.
Claim 2
Dependent Claim
129 words
A process for obtaining purified protein isolates from protein substances containing compounds having a mucilaginous consistency which comprises: (a) making a suspension of cake of such protein substance with a solution of a calcium salt and a magnesium salt of volume in 1. of 10 to 15 times the weight in Kg. of press cake which solution further contains sodium sulphite, (b) adding thereto a concentrated solution of alkali in amount sufficient to solubilize the proteins in said press cake, (c) separating the protein extract from the insoluble residue, (d) acidifying the protein extract obtained with an acid solution at a pH between 4.5 and 5.5 to precipitate the proteins, (e) separating the protein precipitate from the mother liquor, (f) washing the protein precipitate and recovering the protein isolate.
Claim 3
Dependent Claim
47 words
A process according to claim 1 wherein the alkaline earth metal salt solution comprises a calcium salt selected from the group consisting of calcium acetate, calcium lactate, and calcium chloride and a magnesium salt selected from the group consisting of magnesium acetate, magnesium lactate and magnesium chloride.
Claim 4
Dependent Claim
19 words
A process according to claim 1, wherein the protein substance containing compounds having a mucilaginous consistency is sunflower seed.
Claim 5
Dependent Claim
24 words
A process according to claim 1, wherein the alkali is selected from the group consisting of sodium hydroxide, potassium hydroxide and an ammoniacal solution.
Claim 6
Dependent Claim
58 words
A process according to claim 1, wherein, to acidify the protein extract, there is used a solution selected from the group consisting of a normal sulphuric acid solution, a normal hydrochloric acid solution and a solution of another acid making it possible to lower the pH of the protein extract to a value of between 4.5 and 5.5.
Claim 7
Dependent Claim
34 words
A process according to claim 5, wherein the said solution is selected from the group consisting of a phosphoric acid solution, an acetic acid solution, a lactic acid solution or a citric acid solution.
Claim 8
Dependent Claim
14 words
A process according to claim 1, which further comprises drying the washed protein precipitate.
Claim 9
Dependent Claim
30 words
A process according to claim 1, wherein the sodium sulphite is used in amounts such that the concentration of Na sulphite in the mixture is between 1 and 2 g/l.
Claim 10
Dependent Claim
18 words
A process according to claim 1, wherein the Na sulphite is fed together with the concentrated alkali solution.
Claim 11
Dependent Claim
161 words
A process for producing purified sunflower seed protein isolates, which comprises: (a) forming a suspension in a solution of a Ca salt and a magnesium salt further containing a sodium sulphite with sunflower cake ground to a mesh size smaller than 1 mm, the volume in 1 of solution used being 10 to 15 times the weight in kg of the cake; (b) adding to the said suspension an alkali in an amount such that the pH of said suspension is between 7 and 8.5, and leaving the mixture to macerate for about 15 to 30 minutes with stirring; (c) adjusting the pH of the suspension to a value between 10.5 and 12.5, (d) leaving the solution obtained in (c) to macerate with continuous or intermittent stirring; (e) separating the insoluble residue from the solution, and acidifying the resulting solution to a pH of between 4.5 and 5.5 to precipitate the proteins, the proteins precipitated being then separated from the mother-liquor.
Claim 12
Dependent Claim
161 words
A process for producing purified sunflower seed protein isolates, which comprises: (a) forming a suspension in a solution of a Ca salt and a magnesium salt further containing a sodium sulphite with sunflower cake ground to a mesh size smaller than 1 mm, the volume in 1 of solution used being 10 to 15 times the weight in kg of the cake; (b) adding to the said suspension an alkali in an amount such that the pH of said suspension is between 7 and 8.5, and leaving the mixture to macerate for about 15 to 30 minutes with stirring; (c) adjusting the pH of the suspension to a value between 10.5 and 12.5, (d) leaving the solution obtained in (c) to macerate with continuous or intermittent stirring; (e) separating the insoluble residue from the solution, and acidifying the resulting solution to a pH of between 4.5 and 5.5 to precipitate the proteins, the proteins precipitated being then separated from the mother-liquor.
Claim 13
Dependent Claim
24 words
A process according to claim 10, wherein the calcium salt and/or magnesium salt is selected from the group consisting of lactate, acetate and chloride.
Claim 14
Dependent Claim
25 words
A process according to claim 1, wherein the said steps are carried out at a temperature in the range of about 15 to 50° C.
Claim 15
Dependent Claim
16 words
A process according to claim 1, wherein the said steps are carried out at ambient temperature.
Claim 16
Dependent Claim
16 words
A process according to claim 10, wherein the said steps are carried out at ambient temperature.
Claim 17
Dependent Claim
23 words
A process according to claim 3, wherein the sunflower seed cake is ground to a mean mesh size substantially smaller than 1 mm.
Claim 18
Dependent Claim
24 words
A process according to claim 10, wherein the alkali is selected from the group consisting of sodium hydroxide, potassium hydroxide and an ammoniacal solution.
Claim 19
Dependent Claim
58 words
A process according to claim 10, wherein, to acidify the protein extract, there is used a solution selected from the group consisting of a normal sulphuric acid solution, a normal hydrochloric acid solution and a solution of another acid making it possible to lower the pH of the protein extract to a value of between 4.5 and 5.5.
Claim 20
Dependent Claim
34 words
A process according to claim 17, wherein the said solution is selected from the group consisting of a phosphoric acid solution, an acetic acid solution, a lactic acid solution or a citric acid solution.
Claim 21
Dependent Claim
13 words
A process according to claim 10, which further comprises drying the precipitated proteins.
Claim 22
Dependent Claim
30 words
A process according to claim 10, wherein the sodium sulphite is used in amounts such that the concentration of Na sulphite in the mixture is between 1 and 2 g/l.
Claim 23
Dependent Claim
18 words
A process according to claim 10, wherein the Na sulphite is fed together with the concentrated alkali solution.
Claim 24
Dependent Claim
160 words
A process for obtaining purified sunflower seed protein isolates, which comprises: (a) forming a slurry in a solution of a calcium salt and a magnesium salt with sunflower cake ground to a mesh size smaller than 1 mm, the volume of solution in 1. being 3 to 5 times the weight in kg of the cake; (b) adding to said slurry sodium sulphite and a concentrated alkali in an amount such that the normality of said alkali be between 0.10 and 0.15 N, and leaving the mixture to macerate for about 15 to 30 minutes with stirring; (c) adjusting the pH of the solution to a value between 10.5 and 12.5; (d) leaving the solution obtained in (c) to macerate with continuous or intermittent mixing; (e) separating the insoluble residue from the solution and acidifying the resulting solution to a pH of between 4.5 and 5.5 to precipitate the proteins and then separating the precipitated proteins from the mother liquor.
Claim 25
Dependent Claim
160 words
A process for obtaining purified sunflower seed protein isolates, which comprises: (a) forming a slurry in a solution of a calcium salt and a magnesium salt with sunflower cake ground to a mesh size smaller than 1 mm, the volume of solution in 1. being 3 to 5 times the weight in kg of the cake; (b) adding to said slurry sodium sulphite and a concentrated alkali in an amount such that the normality of said alkali be between 0.10 and 0.15 N, and leaving the mixture to macerate for about 15 to 30 minutes with stirring; (c) adjusting the pH of the solution to a value between 10.5 and 12.5; (d) leaving the solution obtained in (c) to macerate with continuous or intermittent mixing; (e) separating the insoluble residue from the solution and acidifying the resulting solution to a pH of between 4.5 and 5.5 to precipitate the proteins and then separating the precipitated proteins from the mother liquor.
Claim 26
Dependent Claim
22 words
A process according to claim 22 wherein the pH of the alkali suspension is adjusted to a pH between 11 and 12.
Claim 27
Dependent Claim
24 words
A process according to claim 22, wherein the alkali is selected from the group consisting of sodium hydroxide, potassium hydroxide and an ammoniacal solution.
Claim 28
Dependent Claim
59 words
A process according to claim 22, wherein the acidification is carried out with a solution of acid selected from the group consisting of normal hydrochloric acid solution, normal sulphuric acid solution, phosphoric acid solution, acetic acid solution, lactic acid solution and citric acid solution to lower the pH of the protein extract to a value between 4.5 and 5.5.
Claim 29
Dependent Claim
15 words
A process according to claim 22, wherein the separated protein precipitate is washed and dried.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-12-25 | 1982-06-26 | 1982-12-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-12-25 | 1986-06-26 | 1986-12-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-12-25 | 1990-06-26 | 1990-12-26 | $7700.00 | Window Closed / Lapsed |