US4401122A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Cutaneous methods of measuring body substances
Assignee: Cincinnati Children's Hospital Medical Center
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Filing: 1979-08-02
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Granted: 1983-08-30
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Expires: 1999-08-02
Abstract
Abstract Cutaneous methods for measurement of substrates in mammalian subjects are disclosed. A condition of the skin is used to measure a number of important substances which diffuse through the skin or are present underneath the skin in the blood or tissue. According to the technique, an enzyme whose activity is specific for a particular substance or substrate is placed on, in or under the skin for reaction. The condition of the skin is then detected by suitable means as a measure of the amount of the substrate in the body. For instance, the enzymatic reaction product or by-product of the reaction is detected directly through the skin as a measure of the amount of substrate. Polarographic electrodes or enzyme electrodes are employed as skin-contact analyzers in the transcutaneous measurement of oxygen or hydrogen peroxide to quantitatively determine blood substances such as glucose and alcohol. In a preferred quantitative technique, the skin is arterialized, i.e., heated or otherwise treated to arterialize the skin capillaries when the measurements are made. Colorimetric detection methods are also employed.
Inventors
- 👤 Leland C. Clark, Jr.
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 20:16
CPC Classifications
A; A61; A61B; A61B5/00; A61B5/145; A61B5/14532; A61B5/1468; A61B5/1477; A61B5/1486; C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/005
Claims Analysis
Total Claims: 41
Claim 1
Independent Claim
64 words
A non-invasive cutaneous method of analyzing body substrates in the body which comprises contacting in the body of non-gaseous body substrate with an enzyme selected for the substrate being analyzed, reacting the substrate with the enzyme, and detecting a condition of the skin without invading the body at the time of the detection as a measure of the amount of substrate in the body.
Claim 2
Dependent Claim
64 words
A non-invasive cutaneous method of analyzing body substrates in the body which comprises contacting in the body of non-gaseous body substrate with an enzyme selected for the substrate being analyzed, reacting the substrate with the enzyme, and detecting a condition of the skin without invading the body at the time of the detection as a measure of the amount of substrate in the body.
Claim 3
Dependent Claim
44 words
The method of claim 1 comprising placing an oxygen sensitive electrode in contact with the skin, reacting the substrate with an enzyme to produce a decrease in oxygen and detecting said oxygen decrease at the skin as a measure of the amount of substrate.
Claim 4
Dependent Claim
32 words
The method of claim 1 comprising locating the enzyme under the skin surface and detecting a condition of the enzymatic reaction at the skin as a measure of the amount of substrate.
Claim 5
Dependent Claim
9 words
The method of claim 1 comprising arterializing the skin.
Claim 6
Dependent Claim
12 words
The method of claim 4 wherein the skin is arterialized by heating.
Claim 7
Dependent Claim
17 words
The method of claim 5 wherein the skin is warmed to a temperature of about 38°-44° C.
Claim 8
Dependent Claim
13 words
The method of claim 4 wherein the skin is arterialized by chemical treatment.
Claim 9
Dependent Claim
10 words
The method of claim 1 comprising injecting the enzyme subcutaneously.
Claim 10
Dependent Claim
28 words
The method of claim 8 comprising mixing the enzyme with a material selected from the group consisting of silicone oil and fluorocarbon oil and injecting the mixture subcutaneously.
Claim 11
Dependent Claim
10 words
The method of claim 1 comprising subcutaneously implanting the enzyme.
Claim 12
Dependent Claim
22 words
The method of claim 10 comprising embedding the enzyme in a thin polymeric sheet and implanting through an incision in the skin.
Claim 13
Dependent Claim
13 words
The method of claim 10 comprising implanting a film containing an immobilized enzyme.
Claim 14
Dependent Claim
19 words
The method of claim 12 wherein the enzyme is immobilized by entrapment between two layers of a plastic film.
Claim 15
Dependent Claim
23 words
The method of claim 12 wherein the film comprises a polymer selected from the group consisting of cellophane, cupraphane, collagen and silicone polymer.
Claim 16
Dependent Claim
23 words
The method of claim 12 wherein enzyme is immobilized on the surface of the film by treating with gluteraldehyde solution and then drying.
Claim 17
Dependent Claim
19 words
The method of claim 1 comprising immobilizing the enzyme in a container which is selectively permeable for the substrate.
Claim 18
Dependent Claim
70 words
A non-invasive cutaneous method of extracorporeally analyzing blood substrates in the body of mammalian subjects which comprises transcutaneously contacting in the body a non-gaseous substrate with an enzyme selected for the subtrate being analyzed, reacting the substrate with the enzyme, arterializing the skin, and detecting the condition of the skin without invading the body at the time of detection as a measure of the amount of substrate in the blood.
Claim 19
Dependent Claim
70 words
A non-invasive cutaneous method of extracorporeally analyzing blood substrates in the body of mammalian subjects which comprises transcutaneously contacting in the body a non-gaseous substrate with an enzyme selected for the subtrate being analyzed, reacting the substrate with the enzyme, arterializing the skin, and detecting the condition of the skin without invading the body at the time of detection as a measure of the amount of substrate in the blood.
Claim 20
Dependent Claim
22 words
The method of claim 17 comprising placing the enzyme in a container under the skin which is selectively permeable for the substrate.
Claim 21
Dependent Claim
22 words
The method of claim 17 comprising detecting either oxygen decrease with an extracorporeal electrode as a measure of the amount of substrate.
Claim 22
Independent Claim
61 words
An apparatus for non-invasively measuring body substrates in the body which comprises means for contacting in the body a non-gaseous body substrate with an enzyme selected for the substrate being analyzed and means for detecting a condition of the skin without invading the body at the time of the detection as a measure of the amount of substrate in the body.
Claim 23
Independent Claim
61 words
An apparatus for non-invasively measuring body substrates in the body which comprises means for contacting in the body a non-gaseous body substrate with an enzyme selected for the substrate being analyzed and means for detecting a condition of the skin without invading the body at the time of the detection as a measure of the amount of substrate in the body.
Claim 24
Dependent Claim
14 words
The apparatus of claim 20 wherein the detecting means is a oxygen sensitive electrode.
Claim 25
Dependent Claim
16 words
The apparatus of claim 20 wherein said detection means is for direct contact with the skin.
Claim 26
Dependent Claim
18 words
The apparatus of claim 20 wherein the contacting means is for location under the surface of the skin.
Claim 27
Dependent Claim
11 words
The apparatus of claim 20 including means for arterializing the skin.
Claim 28
Dependent Claim
9 words
The apparatus of claim 24 comprising a heating element.
Claim 29
Dependent Claim
17 words
The apparatus of claim 25 wherein said element is operated at a temperature of about 38°-44° C.
Claim 30
Dependent Claim
13 words
The apparatus of claim 23 wherein the contacting means comprises an enzyme implant.
Claim 31
Dependent Claim
18 words
The apparatus of claim 27 wherein the enzyme implant comprises an enzyme embedded in a thin polymeric sheet.
Claim 32
Dependent Claim
16 words
The apparatus of claim 27 wherein the enzyme implant comprises a film containing an immobilized enzyme.
Claim 33
Dependent Claim
17 words
The apparatus of claim 29 wherein the enzyme is immobilized between two layers of a plastic film.
Claim 34
Dependent Claim
23 words
The apparatus of claim 30 wherein the film comprises a polymer selected from the group consisting of cellophane, cuprophane, collagen and silicone polymer.
Claim 35
Dependent Claim
26 words
The apparatus of claim 20 wherein the contacting means is a container having an immobilized enzyme therein and said container is selectively permeable for the substrate.
Claim 36
Dependent Claim
21 words
The apparatus of claim 20 comprising further means for vaporizing the substrate and wherein the detecting means is an extracorporeal device.
Claim 37
Dependent Claim
47 words
A detection device for analyzing body substrates in the body which comprises an enzyme immobilized in a layer for implantation in the body and for measurement of body substrates in the body without invading the body at the time of measurement while said enzyme layer is implanted.
Claim 38
Dependent Claim
47 words
A detection device for analyzing body substrates in the body which comprises an enzyme immobilized in a layer for implantation in the body and for measurement of body substrates in the body without invading the body at the time of measurement while said enzyme layer is implanted.
Claim 39
Dependent Claim
18 words
The device of claim 34 wherein said enzyme is immobilized by being embedded in a thin polymeric sheet.
Claim 40
Dependent Claim
16 words
The device of claim 34 wherein the enzyme is immobilized between two layers of plastic film.
Claim 41
Dependent Claim
22 words
The device of claim 34 wherein the enzyme is immobilized within a container and said container being selectively permeable for the substrate.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-08-29 | 1987-02-28 | 1987-08-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-08-29 | 1991-02-28 | 1991-08-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-08-29 | 1995-02-28 | 1995-08-30 | $7700.00 | Window Closed / Lapsed |