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US4396477A
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Separation of proteins using electrodialysis-isoelectric focusing combination
Assignee: Veolia WTS Systems USA Inc
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Filing: 1981-06-29
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Granted: 1983-08-02
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Expires: 2001-06-29
Abstract
Abstract A process and apparatus for treating a liquid protein mixture is disclosed in which the protein mixture is first determined by electrodialysis (ED) and then subjected to isoelectric focusing (IEF) to effect the separation of proteins based on differences in their isoelectric points (pI). This combination of ED-IEF results in an improved separation of the protein components of a mixture than without the ED step.
Inventors
- 👤 Surendar M. Jain
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 02:28
CPC Classifications
B; B01; B01D; B01D61/00; B01D61/42; B01D61/44; B01D57/00; B01D57/02; G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44756; G01N27/44773; G01N27/44795
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
107 words
A process for the treatment of a liquid mixture of proteins comprising the steps of: first demineralizing said protein mixture by subjecting the same to electrodialysis at a temperature of between 0°-40° C. to effect a substantial reduction in the salt content of said mixture to a conductivity in the range between about 0.25-2.0 milli-Siemen/cm; removing from said demineralized mixture any turbidity present, and thereafter subjecting said resulting demineralized protein mixture to isoelectric focusing to effect therefrom a seperation and a simultaneous increase in the relative percentage of at least one of said proteins in at least one channel or separation path of said isoelectric focusing apparatus.
Claim 2
Dependent Claim
107 words
A process for the treatment of a liquid mixture of proteins comprising the steps of: first demineralizing said protein mixture by subjecting the same to electrodialysis at a temperature of between 0°-40° C. to effect a substantial reduction in the salt content of said mixture to a conductivity in the range between about 0.25-2.0 milli-Siemen/cm; removing from said demineralized mixture any turbidity present, and thereafter subjecting said resulting demineralized protein mixture to isoelectric focusing to effect therefrom a seperation and a simultaneous increase in the relative percentage of at least one of said proteins in at least one channel or separation path of said isoelectric focusing apparatus.
Claim 3
Dependent Claim
20 words
The process of claim 1 where the isoelectric focusing process employs recirculation of said protein mixture through said focusing apparatus.
Claim 4
Dependent Claim
18 words
The process of claim 1 whereby the isoelectric focusing is carried out with the use of carrier ampholytes.
Claim 5
Dependent Claim
33 words
The process of claim 1 whereby the isoelectric focusing is carried out by forming a pH gradient by non-mobile charged or chargeable groups immobilized in a matrix along the separation path or channel.
Claim 6
Dependent Claim
40 words
The process of claim 1 whereby the isoelectric focusing is carried out by forming a pH gradient by non-mobile charged or chargeable groups immobilized at least on the surface of the membranes forming the channels of the isoelectric focusing cell.
References Cited (Backward Citations)
US2694680A
Cited
US4204929A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-08-01 | 1987-01-31 | 1987-08-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-08-01 | 1991-01-31 | 1991-08-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-08-01 | 1995-01-31 | 1995-08-02 | $7700.00 | Window Closed / Lapsed |