US4391905A
Expired / Lapsed
US
⏱️ 0.0 years remaining
System for the determination of glucose in fluids
Assignee: Bayer Corp
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Filing: 1982-03-15
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Granted: 1983-07-05
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Expires: 2002-03-15
Abstract
Abstract The present invention involves an improved test system for the determination of glucose in aqueous fluids of the type which comprises glucose oxidase, a peroxidatively active substance and chromogen. The improvement involves the use of m-anisidine as a chromogen which permits the semi-quantitative determination of glucose at higher concentrations than is possible with previously used chromogens.
Inventors
- 👤 Robert Bauer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 04:16
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/54; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/81; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333; Y10T436/144444
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
107 words
A method for the semi-quantitative determination of glucose in urine which comprises contacting the urine with a test strip comprising a bibulous carrier matrix bearing the residue left by impregenating it with a liquid reagent solution comprised of a solvent, glucose oxidase, a peroxidatively active substance and m-anisidine with subsequent evaporation of the solvent to leave the dry reagent with the reagent components being present in the carrier matrix in amounts and proportions such that the carrier matrix is capable of differentiating between concentrations of 2,000 and 5,000 milligrams of glucose per deciliter, and determining the amount of glucose present from the color change in the reagent.
Claim 2
Independent Claim
107 words
A method for the semi-quantitative determination of glucose in urine which comprises contacting the urine with a test strip comprising a bibulous carrier matrix bearing the residue left by impregenating it with a liquid reagent solution comprised of a solvent, glucose oxidase, a peroxidatively active substance and m-anisidine with subsequent evaporation of the solvent to leave the dry reagent with the reagent components being present in the carrier matrix in amounts and proportions such that the carrier matrix is capable of differentiating between concentrations of 2,000 and 5,000 milligrams of glucose per deciliter, and determining the amount of glucose present from the color change in the reagent.
Claim 3
Dependent Claim
42 words
The method of claim 1 wherein the liquid reagent composition contains from 1,000 to 1,600 international units of glucose oxidase per milliliter, 600 to 1,300 international units of horseradish peroxidase per milliliter and from 10 to 15 milligrams per milliliter of m-anisidine.
Claim 4
Dependent Claim
25 words
The method of claim 2 wherein the solution contains from 0.5 to 1.0 milligram per milliliter of a second chromogen having a high extinction coefficient.
Claim 5
Dependent Claim
15 words
The method of claim 3 wherein the second chromogen is benzidine, syringaldazine, diaminofluorine or o-tolidine.
Claim 6
Dependent Claim
11 words
The method of claim 3 wherein the second chromogen is tetramethylbenzidine.
References Cited (Backward Citations)
US3008879A
Cited
US3411887A
Cited
US3630847A
Cited
US3814668A
Cited
US3964870A
Cited
GB1464359A
Cited
US4148611A
Cited
US4260679A
Cited
US4279993A
Cited
US4278439A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-07-04 | 1987-01-03 | 1987-07-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-07-04 | 1991-01-03 | 1991-07-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-07-04 | 1995-01-03 | 1995-07-05 | $7700.00 | Window Closed / Lapsed |