US4279993A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Indicator composition and test device containing amine oxide, and method of use
Assignee: Individual
•
Filing: 1979-11-13
•
Granted: 1981-07-21
•
Expires: 1999-11-13
Abstract
Abstract An improved composition and test device for determining the presence of a constituent in a test sample are disclosed. The composition, which contains a benzidine-type indicator is improved by additionally comprising an amine oxide compound. The device comprises a carrier matrix incorporated with the improved composition. The method comprises contacting the test sample with the composition and observing any detectable response.
Inventors
- 👤 Thomas A. Magers
- 👤 David L. Tabb
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 13:20
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; C12Q1/54; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; C12Q2326/00; C12Q2326/10; C12Q2326/14; Y; Y10; Y10S; Y10S436/00; Y10S436/805; Y10S436/807; Y10S436/81; Y10S436/826
Claims Analysis
Total Claims: 48
Claim 1
Independent Claim
77 words
In a composition for detecting the presence of a constituent in a test sample wherein said composition comprises a benzidine-type indicator having the structure ##STR4## in which the R and R* substituents, same or different, are hydrogen, lower alkyl, lower alkyloxy, aryl, or aryloxy, or in which the R* substituents together comprise --CH 2 -- n in which n is 1 or 2, the improvement wherein said composition additionally comprises, as an enhancer, an amine oxide compound.
Claim 2
Dependent Claim
77 words
In a composition for detecting the presence of a constituent in a test sample wherein said composition comprises a benzidine-type indicator having the structure ##STR4## in which the R and R* substituents, same or different, are hydrogen, lower alkyl, lower alkyloxy, aryl, or aryloxy, or in which the R* substituents together comprise --CH 2 -- n in which n is 1 or 2, the improvement wherein said composition additionally comprises, as an enhancer, an amine oxide compound.
Claim 3
Dependent Claim
23 words
The improved composition of claim 1 wherein said amine oxide is trimethylamine oxide, triethanolamine oxide, pyridine-1-oxide, 3-hydroxypyridine-1-oxide, 4-methylpyridine-1-oxide, 2-chloropyridine-1-oxide, poly(4-vinylpyridine-1-oxide), poly(2-vinylpyridine-1-oxide) or 4,4'-azobis(pyridine-1-oxide).
Claim 4
Dependent Claim
12 words
The improved composition of claim 1 wherein said amine oxide is 2-chloropyridine-1-oxide.
Claim 5
Dependent Claim
12 words
The improved composition of claim 1 wherein said amine oxide is 3-hydroxypyridine-1-oxide.
Claim 6
Dependent Claim
15 words
The improved composition of any of claims 1-3 or 4 wherein said indicator is benzidine.
Claim 7
Dependent Claim
13 words
The improved composition of claim 5 which additionally comprises glucose oxidase and peroxidase.
Claim 8
Dependent Claim
12 words
The improved composition of claim 5 which additionally comprises an organic hydroperoxide.
Claim 9
Dependent Claim
15 words
The improved composition of any of claims 1-3 or 4 wherein said indicator is o-tolidine.
Claim 10
Dependent Claim
13 words
The improved composition of claim 8 which additionally comprises glucose oxidase and peroxidase.
Claim 11
Dependent Claim
12 words
The improved composition of claim 8 which additionally comprises an organic hydroperoxide.
Claim 12
Dependent Claim
15 words
The improved composition of any of claims 1-3 or 4 wherein said indicator is 3,3',5,5'-tetramethylbenzidine.
Claim 13
Dependent Claim
13 words
The improved composition of claim 11 which additionally comprises glucose oxidase and peroxidase.
Claim 14
Dependent Claim
12 words
The improved composition of claim 11 which additionally comprises an organic hydroperoxide.
Claim 15
Dependent Claim
17 words
The improved composition of any of claims 1-3 or 4 which additionally comprises glucose oxidase and peroxidase.
Claim 16
Dependent Claim
16 words
The improved composition of any of claims 1-3 or 4 which additionally comprises an organic hydroperoxide.
Claim 17
Dependent Claim
87 words
In a test device for detecting the presence of a constituent in a test sample wherein said device comprises a carrier matrix incorporated with a composition which comprises a benzidine-type indicator having the structure ##STR5## in which the R and R* substituents, same or different, are hydrogen, lower alkyl, lower alkyloxy, aryl, or aryloxy, or in which the R* substituents together comprise --CH 2 -- n in which n is 1 or 2, the improvement wherein said composition additionally comprises, as an enhancer, an amine oxide compound.
Claim 18
Dependent Claim
87 words
In a test device for detecting the presence of a constituent in a test sample wherein said device comprises a carrier matrix incorporated with a composition which comprises a benzidine-type indicator having the structure ##STR5## in which the R and R* substituents, same or different, are hydrogen, lower alkyl, lower alkyloxy, aryl, or aryloxy, or in which the R* substituents together comprise --CH 2 -- n in which n is 1 or 2, the improvement wherein said composition additionally comprises, as an enhancer, an amine oxide compound.
Claim 19
Dependent Claim
24 words
The improved test device of claim 16 wherein said amine oxide is trimethylamine oxide, triethanolamine oxide, pyridine-1-oxide, 3-hydroxypyridine-1-oxide, 4-methylpyridine-1-oxide, 2-chloropyridine-1-oxide, poly(4-vinylpyridine-1-oxide), poly(2-vinylpyridine-1-oxide) or 4,4'-azobis(pyridine-1-oxide).
Claim 20
Dependent Claim
13 words
The compound test device of claim 16 wherein said amine oxide is 2-chloropyridine-1-oxide.
Claim 21
Dependent Claim
13 words
The improved test device of claim 16 wherein said amine oxide is 3-hydroxypyridine-1-oxide.
Claim 22
Dependent Claim
16 words
The improved test device of any of claims 16-18 or 19 wherein said indicator is benzidine.
Claim 23
Dependent Claim
14 words
The improved test device of claim 20 which additionally comprises glucose oxidase and peroxidase.
Claim 24
Dependent Claim
13 words
The improved test device of claim 20 which additionally comprises an organic hydroperoxide.
Claim 25
Dependent Claim
16 words
The improved test device of any of claims 16-18 or 19 wherein said indicator is o-tolidine.
Claim 26
Dependent Claim
14 words
The improved test device of claim 23 which additionally comprises glucose oxidase and peroxidase.
Claim 27
Dependent Claim
13 words
The improved test device of claim 23 which additionally comprises an organic hydroperoxide.
Claim 28
Dependent Claim
16 words
The improved test device of any of claims 16-18 or 19 wherein said indicator is 3,3',5,5'-tetramethylbenzidine.
Claim 29
Dependent Claim
14 words
The improved test device of claim 26 which additionally comprises glucose oxidase and peroxidase.
Claim 30
Dependent Claim
13 words
The improved test device of claim 26 which additionally comprises an organic hydroperoxide.
Claim 31
Dependent Claim
18 words
The improved test device of any of claims 16-18 or 19 which additionally comprises glucose oxidase and peroxidase.
Claim 32
Dependent Claim
17 words
The improved test device of any of claims 16-18 or 19 which additionally comprises an organic hydroperoxide.
Claim 33
Independent Claim
82 words
A method for determining the presence of a constituent in a test sample, said method comprising the steps of contacting the sample with a composition which comprises a benzidene-type indicator having the structure ##STR6## in which the R and R* substituents, same or different, are hydrogen, lower alkyl, lower alkoxy, aryl, or aryloxy, or in which the R* substituents together comprise --CH 2 -- n in which n is 1 or 2, and an amine oxide compound, and observing any detectable response.
Claim 34
Independent Claim
82 words
A method for determining the presence of a constituent in a test sample, said method comprising the steps of contacting the sample with a composition which comprises a benzidene-type indicator having the structure ##STR6## in which the R and R* substituents, same or different, are hydrogen, lower alkyl, lower alkoxy, aryl, or aryloxy, or in which the R* substituents together comprise --CH 2 -- n in which n is 1 or 2, and an amine oxide compound, and observing any detectable response.
Claim 35
Dependent Claim
22 words
The method of claim 31 wherein said amine oxide is trimethylamine oxide, triethanolamine oxide, pyridine-1-oxide, 3-hydroxypyridine-1-oxide, 4-methylpyridine-1-oxide, 2-chloropyridine-1-oxide, poly(4-vinylpyridine-1-oxide), poly(2-vinylpyridine-1-oxide) or 4,4'-azobis(pyridine-1-oxide).
Claim 36
Dependent Claim
11 words
The method of claim 31 wherein said amine oxide is 2-chloropyridine-1-oxide.
Claim 37
Dependent Claim
11 words
The method of claim 31 wherein said amine oxide is 3-hydroxypridine-1-oxide.
Claim 38
Independent Claim
14 words
The method of any of claims 31-33 or 34 wherein said indicator is benzidine.
Claim 39
Dependent Claim
14 words
The method of claim 35 wherein said composition additionally comprises glucose oxidase and peroxidase.
Claim 40
Dependent Claim
13 words
The method of claim 35 wherein said composition addtionally comprises an organic hydroperoxide.
Claim 41
Independent Claim
14 words
The method of any of claims 31-33 or 34 wherein said indicator is o-tolidine.
Claim 42
Dependent Claim
14 words
The method of claim 38 wherein said composition additionally comprises glucose oxidase and peroxidase.
Claim 43
Dependent Claim
13 words
The method of claim 38 wherein said composition additionally comprises an organic hydroperoxide.
Claim 44
Independent Claim
15 words
The method of any of claims 31-33 or 34 wherein said indicator is 3,3',5,5' tetramethylbenzidine.
Claim 45
Dependent Claim
14 words
The method of claim 41 wherein said composition additionally comprises glucose oxidase and peroxidase.
Claim 46
Dependent Claim
13 words
The method of claim 41 wherein said composition additionally comprises an organic hydroperoxide.
Claim 47
Independent Claim
18 words
The method of any of claims 31-33 or 34 wherein said composition additionally comprises glucose oxidase and peroxidase.
Claim 48
Independent Claim
17 words
The method of any of claims 31-33 or 34 wherein said composition additionally comprises an organic hydroperoxide.
References Cited (Backward Citations)
US4071317A
Cited
US4071321A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-07-20 | 1985-01-19 | 1985-07-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-07-20 | 1989-01-19 | 1989-07-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-07-20 | 1993-01-19 | 1993-07-21 | $7700.00 | Window Closed / Lapsed |