Patent US4390633A successfully refreshed from Google Patents.
US4390633A
Expired / Lapsed
US
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Determining reproductive status of mammals with NMR of cervical mucus
Assignee: University of Missouri-Columbia
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Filing: 1981-07-24
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Granted: 1983-06-28
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Expires: 2001-07-24
Abstract
Abstract A method for determining the reproductive status of mammals involves the steps of obtaining at periodic time intervals mucus samples from the cervix of a mammal, obtaining nuclear magnetic resonance spectra of the samples using a narrow line nuclear magnetic resonance spectrometer, determining the nuclear magnetic resonance ratios from the spectra on the basis of the relationship <IMAGE> and thereafter evaluating the reproductive status of the mammal from such ratios.
Inventors
- 👤 Charles P. Merilan
- 👤 Jerald R. Sears
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 03:51
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01R; G01R33/00; G01R33/20; G01R33/44; G01R33/46; G01R33/465; Y; Y10; Y10S; Y10S436/00; Y10S436/906; Y10T; Y10T436/00; Y10T436/24
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
75 words
The method for determining pregnancy or reproductive status in a mammal which comprises the steps of: a. obtaining at periodic time intervals mucus samples from the cervix of a mammal whose reproductive status is to be determined; b. obtaining nuclear magnetic resonance spectra of said samples; c. determining the nuclear magnetic resonance ratios from said spectra on the basis of the relationship ##EQU6## and d. evaluating the reproductive status of said mammal from said ratios.
Claim 2
Independent Claim
75 words
The method for determining pregnancy or reproductive status in a mammal which comprises the steps of: a. obtaining at periodic time intervals mucus samples from the cervix of a mammal whose reproductive status is to be determined; b. obtaining nuclear magnetic resonance spectra of said samples; c. determining the nuclear magnetic resonance ratios from said spectra on the basis of the relationship ##EQU6## and d. evaluating the reproductive status of said mammal from said ratios.
Claim 3
Dependent Claim
31 words
The method of claim 1 wherein the nuclear magnetic resonance spectra are obtained utilizing a nuclear magnetic resonance spectrometer operated at a frequency of between approximately 60 MHz and 200 MHz.
Claim 4
Dependent Claim
20 words
The method of claim 2 wherein the nuclear magnetic resonance spectrometer is operated at a frequency of approximately 60 MHz.
Claim 5
Dependent Claim
18 words
The method of claim 1 wherein the samples obtained from the cervix of said mammal approximate 0.1 ml.
Claim 6
Dependent Claim
29 words
The method of claim 1 wherein said mucus samples are at a temperature of from approximately 4° C. to 40° C. when the nuclear magnetic resonance spectra are obtained.
Claim 7
Dependent Claim
23 words
The method of claim 5 wherein the temperature of said samples is approximately 20° C. when the nuclear magnetic resonance spectra are obtained.
Claim 8
Dependent Claim
30 words
The method of claim 1 wherein the ratio of peak width at half amplitude to peak height of said spectra is at a higher value indicating pregnancy of said mammal.
Claim 9
Dependent Claim
38 words
The method of claim 1 wherein the ratio of peak width at half amplitude to peak height of said spectra is at a low value indicating the onset of estrus or impending termination of pregnancy in said mammal.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-06-27 | 1986-12-27 | 1987-06-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-06-27 | 1990-12-27 | 1991-06-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-06-27 | 1994-12-27 | 1995-06-28 | $7700.00 | Window Closed / Lapsed |