Patent US4388296A successfully refreshed from Google Patents.
US4388296A
Expired / Lapsed
US
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Energy-emitting latex-particulates
Assignee: Individual
•
Filing: 1980-09-29
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Granted: 1983-06-14
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Expires: 2000-09-29
Abstract
Abstract This invention relates to improved energy-emitting latex-particulates for use in a non-destructive method of biological assay, wherein the biological material under assay links the distinct and different particulates in such a manner that their proximity results in a detectable signal which is indicative of the amount of such biological material present in the fluid. More particularly, this invention relates to improved energy-emitting latex-particulates which have improved stability, a greatly extended shelf-life, particularly in the fluid suspension state, and whose use in the quantitative assay of immunological material results in a reduced background signal and improved sensitivity and stability.
Inventors
- 👤 Hiram Hart
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 11:33
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/536; G01N33/542; Y; Y10; Y10S; Y10S436/00; Y10S436/804
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
124 words
Improved energy-emitting latex particulates for use in the non-destructive method of biological fluid assay wherein biological material being assayed links fluid suspensions of both a plurality of tritiated latex particulates and potentially scintillating latex particulates within activating range one to the other, each of said scintillating latex particulates emitting detectable light pulses upon activation responsive to bombardment of energy from each of said linked tritiated latex particulates within said range, said light pulses being quantitatively correlative to the concentration of said biological material in said fluid, the improvement wherein said tritiated latex particulates, having a polystyrene base with benzotriazole, coated covalently with an antibody, and said scintillating latex particulates having a polystyrene base with 2,5-diphenylfuran and p-bis (α-methylstyrl) benzene, coated covalently with an antibody.
Claim 2
Dependent Claim
124 words
Improved energy-emitting latex particulates for use in the non-destructive method of biological fluid assay wherein biological material being assayed links fluid suspensions of both a plurality of tritiated latex particulates and potentially scintillating latex particulates within activating range one to the other, each of said scintillating latex particulates emitting detectable light pulses upon activation responsive to bombardment of energy from each of said linked tritiated latex particulates within said range, said light pulses being quantitatively correlative to the concentration of said biological material in said fluid, the improvement wherein said tritiated latex particulates, having a polystyrene base with benzotriazole, coated covalently with an antibody, and said scintillating latex particulates having a polystyrene base with 2,5-diphenylfuran and p-bis (α-methylstyrl) benzene, coated covalently with an antibody.
Claim 3
Dependent Claim
125 words
Improved energy emitting latex particulates for use in the non-destructive method of biological fluid assay wherein biological material being assayed links fluid suspensions of both a plurality of tritiated latex particulates and potentially scintillating latex particulates within activating range one to the other, each of said scintillating latex particulates emitting detectable light pulses upon activation responsive to bombardment of energy from each of said linked tritiated latex particulates within said range, said light pulses being quantitatively correlative to the concentration of said biological material in said fluid, the improvement wherein said tritiated latex particulates, having a polystyrene base with benzotriazole, coated covalently with an antigen, and said scintillating latex particulates, having a polystyrene base with 2,5-diphenylfuran and p-bis (α-methylstyryl) benzene, coated covalently with an antigen.
Claim 4
Dependent Claim
125 words
Improved energy emitting latex particulates for use in the non-destructive method of biological fluid assay wherein biological material being assayed links fluid suspensions of both a plurality of tritiated latex particulates and potentially scintillating latex particulates within activating range one to the other, each of said scintillating latex particulates emitting detectable light pulses upon activation responsive to bombardment of energy from each of said linked tritiated latex particulates within said range, said light pulses being quantitatively correlative to the concentration of said biological material in said fluid, the improvement wherein said tritiated latex particulates, having a polystyrene base with benzotriazole, coated covalently with an antigen, and said scintillating latex particulates, having a polystyrene base with 2,5-diphenylfuran and p-bis (α-methylstyryl) benzene, coated covalently with an antigen.
Claim 5
Dependent Claim
22 words
Improved energy emitting latex particulates comprising tritiated latex particulates coated covalently with an antibody and having a polystyrene base and containing benzotriazole.
Claim 6
Dependent Claim
22 words
Improved energy emitting latex particulates comprising tritiated latex particulates coated covalently with an antibody and having a polystyrene base and containing benzotriazole.
Claim 7
Dependent Claim
26 words
Improved energy emitting latex particulates comprising scintillating latex particulates coated covalently with an antibody and having a polystyrene base and containing 2,5-diphenylfuran and p-bis (α-methylstyryl) benzene.
Claim 8
Dependent Claim
26 words
Improved energy emitting latex particulates comprising scintillating latex particulates coated covalently with an antibody and having a polystyrene base and containing 2,5-diphenylfuran and p-bis (α-methylstyryl) benzene.
Claim 9
Dependent Claim
22 words
Improved energy emitting latex particulates comprising tritiated latex particulates coated covalently with an antigen and having a polystyrene base and containing benzotriazole.
Claim 10
Dependent Claim
22 words
Improved energy emitting latex particulates comprising tritiated latex particulates coated covalently with an antigen and having a polystyrene base and containing benzotriazole.
Claim 11
Dependent Claim
26 words
Improved energy emitting latex particulates comprising scintillating latex particulates coated covalently with an antigen and having a polystyrene base and containing 2,5-diphenylfuran and p-bis (α-methylstyryl) benzene.
Claim 12
Dependent Claim
26 words
Improved energy emitting latex particulates comprising scintillating latex particulates coated covalently with an antigen and having a polystyrene base and containing 2,5-diphenylfuran and p-bis (α-methylstyryl) benzene.
Claim 13
Dependent Claim
58 words
An improved energy emitting latex particulates comprising a core of separable layers, one of said layers tritiated latex particulates coated covalently with an antibody and having a polystyrene base and containing benzotriazole, the other of said layers potentially scintillating latex particulates coated covalently with an antibody and having a polystryene base and containing 2,5-diphenylfuran and p-bis (α-methylstyryl) benzene.
Claim 14
Dependent Claim
58 words
An improved energy emitting latex particulates comprising a core of separable layers, one of said layers tritiated latex particulates coated covalently with an antibody and having a polystyrene base and containing benzotriazole, the other of said layers potentially scintillating latex particulates coated covalently with an antibody and having a polystryene base and containing 2,5-diphenylfuran and p-bis (α-methylstyryl) benzene.
Claim 15
Dependent Claim
58 words
An improved energy emitting latex particulates comprising a core of separable layers, one of said layers tritiated latex particulates coated covalently with an antigen and having a polystyrene base and containing benzotriazole, the other of said layers potentially scintillating latex particulates coated covalently with an antigen and having a polystryene base and containing 2,5-diphenylfuran and p-bis (α-methylstyryl) benzene.
Claim 16
Dependent Claim
58 words
An improved energy emitting latex particulates comprising a core of separable layers, one of said layers tritiated latex particulates coated covalently with an antigen and having a polystyrene base and containing benzotriazole, the other of said layers potentially scintillating latex particulates coated covalently with an antigen and having a polystryene base and containing 2,5-diphenylfuran and p-bis (α-methylstyryl) benzene.
References Cited (Backward Citations)
US4271139A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-06-13 | 1986-12-13 | 1987-06-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-06-13 | 1990-12-13 | 1991-06-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-06-13 | 1994-12-13 | 1995-06-14 | $7700.00 | Window Closed / Lapsed |