Patent US4387092A successfully refreshed from Google Patents.
US4387092A
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Process for the preparation of factor VIII concentrate
Assignee: Institut Merieux SA
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Filing: 1980-06-30
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Granted: 1983-06-07
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Expires: 2000-06-30
Abstract
Abstract An improved process for preparing factor VIII concentrate which comprises a first stage wherein fibrinogen is precipitated from a buffered plasma or plasma cryoprecipitate solution by the addition of a polyol and a second stage where factor VIII is precipitated from the resulting supernatant also by the addition of a polyol. The improvement in the first stage resides in adding the polyol in an amount of 1-3% and maintaining the temperature of the buffered solution between about 2°-4° C.
Inventors
- 👤 Jacques Liautaud
- 👤 Edward Shanbrom
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 16:59
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/745; C07K14/755; A; A61; A61K; A61K38/00; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
155 words
In a process for preparing Factor VIII concentrate where in a first stage fibrinogen is precipitated from a solution of plasma or plasma cryoprecipitate from a buffered solution having a pH ranging between 6 and 7 by the addition of a polyol and the precipitate comprising fibrinogen is removed to provide a supernatant comprising Factor VIII and in a second stage said Factor VIII is precipitated from said supernatant by increasing the polyol concentration to at least about 6% and holding the solution containing said increased amounts of polyol at from about 0° to about 5° until precipitation of said Factor VIII occurs, the improvement comprising the steps of in said first stage of precipitating fibrinogen from a solution of plasma or plasma cryoprecipitate from said buffered solution by the addition of a polyol in an amount of about 1 to 3% while cooling said solution to a temperature between about 2° and 4° C.
Claim 2
Dependent Claim
155 words
In a process for preparing Factor VIII concentrate where in a first stage fibrinogen is precipitated from a solution of plasma or plasma cryoprecipitate from a buffered solution having a pH ranging between 6 and 7 by the addition of a polyol and the precipitate comprising fibrinogen is removed to provide a supernatant comprising Factor VIII and in a second stage said Factor VIII is precipitated from said supernatant by increasing the polyol concentration to at least about 6% and holding the solution containing said increased amounts of polyol at from about 0° to about 5° until precipitation of said Factor VIII occurs, the improvement comprising the steps of in said first stage of precipitating fibrinogen from a solution of plasma or plasma cryoprecipitate from said buffered solution by the addition of a polyol in an amount of about 1 to 3% while cooling said solution to a temperature between about 2° and 4° C.
Claim 3
Dependent Claim
13 words
The process of claim 1 wherein said pH ranges from 6.0 to 6.5.
Claim 4
Dependent Claim
36 words
The process of claim 1 where in said first stage said buffered solution is stirred for a period of time ranging from 5 minutes to 3 hours while maintaining the temperature thereof at the selected temperature.
Claim 5
Dependent Claim
11 words
The process of claim 1 wherein the polyol is polyethylene glycol.
Claim 6
Dependent Claim
11 words
The process of claim 4 wherein said polyethyleneglycol is polyethyleneglycol 400.
Claim 7
Dependent Claim
16 words
The process of claim 1 wherein said polyol is a sequenced polymer of the polyoxyethylene-polyoxypropylene-polyoxyethylene type.
Claim 8
Dependent Claim
10 words
The process of claim 6 wherein said polyol is Pluronic-68.
References Cited (Backward Citations)
FR1350895A
Cited
FR1589414A
Cited
US3631018A
Cited
US3770631A
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US4069216A
Cited
US4085095A
Cited
FR2348702B1
Cited
FR2363577B1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-06-06 | 1986-12-06 | 1987-06-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-06-06 | 1990-12-06 | 1991-06-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-06-06 | 1994-12-06 | 1995-06-07 | $7700.00 | Window Closed / Lapsed |