US4385974A
Expired / Lapsed
US
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Electrophoretic system and method for multidimensional analysis
Assignee: Individual
•
Filing: 1982-06-24
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Granted: 1983-05-31
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Expires: 2002-06-24
Abstract
Abstract A system for electrophoretic analysis includes four tanks, each of which carries an electrolytic solution which accesses a different edge surface of a pair of parallel gel plates. The gel plates include an electrophoretic separation path formed of a selected medium, e.g. an isoelectric focusing gel, extending in a space between the plates and across opposite edges of the plates. A pair of nonpolar barrier webs extend between the plates parallel to and on each side of the path. A different medium, e.g. a running gel, occupies the areas between the gel plates from the opposite sides of the barriers to the remaining edges of the plates. A pair of gaskets are positioned along plate edges which are perpendicular to the path. The gaskets include access apertures for the path and the barriers. A specimen is positioned within a channel leading from an electrolytic solution tank to the path aperture of a gasket and between a dam and the aperture. Electrophoretic separation of the specimen constituents within the separation path is achieved by current flow between a pair of tanks which communicate with the path through the gasket apertures. The barriers are removed preferably by aspiration after specimen separation. An equilibrating medium and gel are infused into the spaces previously occupied by the barriers and electrophoretic separation into the different medium is commenced by current flow between the remaining tanks which access the edges of the gel plates in communication with the different medium.
Inventors
- 👤 Jerry Shevitz
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 17:39
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44756; G01N27/44773
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
261 words
An apparatus for conducting electrophoretic analysis, the apparatus comprising means for supporting an electrophoresis gel slab, the gel slab including a separation path comprising a first gel and defined by the space between a pair of parallel gel plates and a pair of barrier webs, the gel slab further including a second gel positioned in the space between the gel plates and adjacent at least one barrier web, the apparatus further comprising means forming a first pair of tanks for carrying an electrolytic solution and electrode means communicating with the electrolytic solution in each tank, means accessing the solution of each tank to opposite ends of the separation path, means for accessing the barrier webs for aspiration of the barrier webs from the gel slab after electrophoretic separation of a specimen in the separation path, the accessing means including means for permitting the infusion of an equilibrating medium into the spaces between the gel plates previously occupied by the barrier webs, the apparatus further including a second pair of tanks for carrying an electrolytic solution and electrode means communicating with the solution in each of the tanks of the second pair, means for accessing the solution of each tank of the second pair with the second gel, the specimen particles separated in the separation path being further separated by particle size in the second gel when an electrical potential is applied between the solutions in each of the tanks of the second pair, whereby two dimensional electrophoretic analysis is obtained without the necessity of manipulating the gel of the separation path.
Claim 2
Independent Claim
261 words
An apparatus for conducting electrophoretic analysis, the apparatus comprising means for supporting an electrophoresis gel slab, the gel slab including a separation path comprising a first gel and defined by the space between a pair of parallel gel plates and a pair of barrier webs, the gel slab further including a second gel positioned in the space between the gel plates and adjacent at least one barrier web, the apparatus further comprising means forming a first pair of tanks for carrying an electrolytic solution and electrode means communicating with the electrolytic solution in each tank, means accessing the solution of each tank to opposite ends of the separation path, means for accessing the barrier webs for aspiration of the barrier webs from the gel slab after electrophoretic separation of a specimen in the separation path, the accessing means including means for permitting the infusion of an equilibrating medium into the spaces between the gel plates previously occupied by the barrier webs, the apparatus further including a second pair of tanks for carrying an electrolytic solution and electrode means communicating with the solution in each of the tanks of the second pair, means for accessing the solution of each tank of the second pair with the second gel, the specimen particles separated in the separation path being further separated by particle size in the second gel when an electrical potential is applied between the solutions in each of the tanks of the second pair, whereby two dimensional electrophoretic analysis is obtained without the necessity of manipulating the gel of the separation path.
Claim 3
Dependent Claim
68 words
An apparatus for conducting electrophoretic analysis constructed in accordance with claim 1 wherein the means for supporting the gel slab includes a generally flat planar platen and a pair of parallel side walls, the means for accessing the barrier webs including means forming slots in the side wall in registry with the barrier webs whereby a syringe may be placed within the slots for aspirating the barrier webs.
Claim 4
Dependent Claim
41 words
An apparatus for conducting electrophoretic analysis constructed in accordance with claim 1 further including a monolithic base, the tanks being formed as depressions in the base and the means for supporting the gel slab comprising a platen formed in the base.
Claim 5
Dependent Claim
148 words
An apparatus for conducting electrophoretic analysis constructed in accordance with claim 1 wherein the means for supporting the electrophoresis gel slab comprises means forming a generally planar platen, the platen including a pair of parallel upstanding side walls, the apparatus further including a pair of elongate compressible gaskets, and means for compressing each gasket between the gel plates and one of the side walls of the platen, the compressing means comprising clamping means positioned adjacent one of the side walls for urging the gel plates toward the other side wall to compress one compressible gasket between the gel plates and the other side wall thereby providing an enlarged space between the gel plates and the one side wall, the space being of a width greater than the thickness of a compressible gasket, the other compressible gasket being positioned in such space and the compressing means being thereafter released.
Claim 6
Dependent Claim
126 words
An apparatus for conducting electrophoresis analysis constructed in accordance with claim 1 wherein the means accessing the solution of the first pair of tanks to opposite ends of the separation path includes means forming a channel between one of the first tanks and one end of the separation path, the apparatus further including means for introducing a specimen into the separation path, the specimen introducing means comprising means forming a dam in the channel, the dam extending to an elevation beneath the elevation of a solution in the channel, the dam being positioned adjacent one end of the separation path, the specimen being positioned between the one end of the separation path and the dam, the solution extending to an elevation above that of the specimen.
Claim 7
Dependent Claim
30 words
An apparatus for conducting electrophoretic analysis constructed in accordance with claim 5 wherein the dam extends to an elevation at least as high as the elevation of the separation path.
Claim 8
Dependent Claim
21 words
An apparatus for conducting electrophoretic analysis constructed in accordance with claim 5 wherein the specimen includes means for increasing its density.
Claim 9
Dependent Claim
154 words
An electrophoresis gel slab suitable for two dimensional electrophoretic analysis, the gel slab comprising a pair of substantially rectangular gel plates having parallel side edges and ends, means maintaining the gel plates uniformly spaced from one another in parallel planes, one gel positioned between the plates, and one gel extending substantially across the side edges of the plates from one end of the plates to a distance less than the distance between the end surfaces of the plates, a first barrier web of nonconducting material positioned between the plates, the first barrier web extending substantially across the side edges in juxtaposed relationship to the one gel, the further gel extending substantially across the side edges of the plates in juxtaposed relationship to the first barrier web, a second barrier web positioned between the plates, the second barrier web extending substantially across the side edges of the plates in juxtaposed relationship to the further gel.
Claim 10
Dependent Claim
154 words
An electrophoresis gel slab suitable for two dimensional electrophoretic analysis, the gel slab comprising a pair of substantially rectangular gel plates having parallel side edges and ends, means maintaining the gel plates uniformly spaced from one another in parallel planes, one gel positioned between the plates, and one gel extending substantially across the side edges of the plates from one end of the plates to a distance less than the distance between the end surfaces of the plates, a first barrier web of nonconducting material positioned between the plates, the first barrier web extending substantially across the side edges in juxtaposed relationship to the one gel, the further gel extending substantially across the side edges of the plates in juxtaposed relationship to the first barrier web, a second barrier web positioned between the plates, the second barrier web extending substantially across the side edges of the plates in juxtaposed relationship to the further gel.
Claim 11
Dependent Claim
41 words
An electrophoresis gel slab constructed in accordance with claim 8 further including an elongate gasket positioned adjacent each of the side edges, and means forming an opening in each of the gaskets in registration with the ends of the further gel.
Claim 12
Dependent Claim
28 words
An electrophoresis gel slab constructed in accordance with claim 9 further including means forming an opening in the gaskets adjacent each of the ends of the barrier webs.
Claim 13
Dependent Claim
34 words
An electrophoresis gel slab constructed in accordance with claim 10 wherein the means maintaining the gel plates uniformly spaced from one another comprises a spine projecting between the plates from each of the gaskets.
Claim 14
Dependent Claim
52 words
An electrophoresis gel slab constructed in accordance with claim 8 further including a third gel, the third gel being positioned in the space between the plates in juxtaposed relationship to the second barrier web and extending substantially across the side edges of the plates to the remaining end of the gel plates.
Claim 15
Dependent Claim
25 words
An electrophoresis gel slab constructed in accordance with claim 8 further including a layer of isobutanol intermediate the one gel and the first barrier web.
Claim 16
Dependent Claim
25 words
An electrophoresis gel slab constructed in accordance with claim 8 wherein the further gel comprises an isoelectric focusing gel and includes a bis-acrylamide supporting matrix.
Claim 17
Dependent Claim
19 words
An electrophoresis gel slab constructed in accordance with claim 8 wherein the further gel includes an agarose supporting matrix.
Claim 18
Dependent Claim
141 words
A method of obtaining a two dimensional electrophoretic analysis of a specimen with a gel slab constructed in accordance with claim 8 comprising the steps of: (a) accessing the opposite ends of the further gel with separate electrolytic solution; (b) accessing the further gel with a specimen; (c) causing the specimen to separate in the further gel by applying an electrical potential across the separate electrolytic solutions; (d) removing the barrier webs from the gel slab by aspiration; (e) infusing an equilibrating medium in the spaces occupied by the barrier webs; (f) forming a stacking gel in the spaces occupied in the barrier webs; (g) accessing the one gel with a buffer solution; and (h) causing the previously separated specimen to migrate into the one gel by applying an electrical potential between the one gel electrolytic solution and the further gel.
Claim 19
Dependent Claim
80 words
A method of obtaining an electrophoretic analysis of a specimen as set forth in claim 16 wherein the gel slab further includes a third gel positioned between the gel plates in juxtaposed relationship to the second barrier web, the method further including the step of accessing the third gel with an electrolytic solution, the electrical potential at the further gel being applied by applying an electrical potential across both the one gel electrolytic solution and the third gel electrolytic solution.
Claim 20
Dependent Claim
35 words
A method of obtaining a two dimensional electrophoretic analysis of a specimen in accordance with claim 16 wherein the barrier webs are aspirated with a syringe and the equilibrating medium is infused with a syringe.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-05-30 | 1986-11-29 | 1987-05-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-05-30 | 1990-11-29 | 1991-05-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-05-30 | 1994-11-29 | 1995-05-31 | $7700.00 | Window Closed / Lapsed |