US4305799A
Expired / Lapsed
US
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Method and apparatus for performing uni- and bi-dimensional micro-gel electrophoresis
Assignee: Max-Planck-Gesellschaft zur Förderung der Wissenschaften eV
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Filing: 1980-07-09
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Granted: 1981-12-15
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Expires: 2000-07-09
Abstract
Abstract The invention provides a method of performing a unidimensional and bidimensional microgel electrophoresis which method comprises first performing a unidimensional microgel electrophoresis in a capillary gel and then removing the capillary gel from its capillary tube, wherein a capillary gel rodlet obtained after said unidimensional microgel electrophoresis is disposed along one side edge of a capillary gel slab and bound to the latter and then performing the second dimension of the bidimensional microgel electrophoresis in the capillary gel slab.
Inventors
- 👤 Uli Schwarz
- 👤 Robert Neukirchen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 22:23
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44756; G01N27/44795; G01N27/44773
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
75 words
Method of performing a unidimensional and bidimensional microgel electrophoresis which method comprises first performing a unidimensional microgel electrophoresis in a capillary gel and then removing the capillary gel from its capillary tube, wherein a capillary gel rodlet obtained after said unidimensional microgel electrophoresis is disposed along one side edge of a capillary gel slab and bound to the latter and then performing the second dimension of the bidimensional microgel electrophoresis in the capillary gel slab.
Claim 2
Dependent Claim
75 words
Method of performing a unidimensional and bidimensional microgel electrophoresis which method comprises first performing a unidimensional microgel electrophoresis in a capillary gel and then removing the capillary gel from its capillary tube, wherein a capillary gel rodlet obtained after said unidimensional microgel electrophoresis is disposed along one side edge of a capillary gel slab and bound to the latter and then performing the second dimension of the bidimensional microgel electrophoresis in the capillary gel slab.
Claim 3
Dependent Claim
26 words
Method as claimed in claim 1 wherein the capillary gel rodlet is bound to the capillary gel slab by being laid directly into the collector gel.
Claim 4
Dependent Claim
33 words
Method as claimed in claim 1 wherein the capillary gel rodlet is laid upon a side edge of the capillary gel slab, which side edge is formed from separating gel and is thickened.
Claim 5
Dependent Claim
27 words
Method as claimed in claim 2 wherein the capillary gel rodlet is bound by means of a buffer solution as a wetting bond to the collector gel.
Claim 6
Dependent Claim
52 words
Method as claimed in claim 2 wherein a capillary gel rodlet of rounded cross section is used and the supporting surface of the collector gel or of the thickened side edge formed of separating gel of the capillary gel slab, on which the capillary gel rodlet is laid, is of concave shape.
Claim 7
Dependent Claim
37 words
Method as claimed in claim 1 wherein the unidimensional microgel electrophoresis is performed in a capillary gel slab on whose one side edge a plurality of samples are applied at intervals extending lengthwise of the side edge.
Claim 8
Dependent Claim
40 words
Method as claimed in claim 6 wherein pockets extending transversely of and preferably perpendicular to the side edge of the capillary gel slab are provided in the capillary gel slab and the individual samples are placed in these individual pockets.
Claim 9
Dependent Claim
23 words
Method as claimed in claim 1 wherein the unidimensional microgel electrophoresis is performed in a rod shaped capillary gel of rounded cross section.
Claim 10
Dependent Claim
20 words
Method as claimed in claim 1 wherein capillary gel slabs of a thickness of less than 500 micrometers are used.
Claim 11
Dependent Claim
20 words
Method as claimed in claim 9 wherein capillary gel slabs of a thickness of 200 to 300 micrometers are used.
Claim 12
Dependent Claim
16 words
Method as claimed in claim 1 wherein the unidimensional microgel electrophoresis is performed with iso-electrical focusing.
Claim 13
Dependent Claim
22 words
Method as claimed in claim 1 wherein the second dimension or bi-dimensional microgel electrophoresis is performed as a sodium dodecyl sulfate electrophoresis.
Claim 14
Dependent Claim
38 words
Method as claimed in claim 1 wherein the first unidimensional microgel electrophoresis is performed first for a predetermined length of time with constant current and then it is performed at constant voltage for the rest of the time.
Claim 15
Dependent Claim
41 words
Method as claimed in claim 13 wherein the second dimension of the bidimensional microgel electrophoresis is performed first for a predetermined length of time with constant current and then it is performed at constant voltage for the rest of the time.
Claim 16
Dependent Claim
26 words
Method as claimed in claim 13 wherein the predetermined length of time amounts to approximately 20 to 30% of the total duration of the microgel electrophoresis.
Claim 17
Dependent Claim
44 words
Method as claimed in claim 13 wherein the microgel electrophoresis is begun at a voltage of 30 to 40 volts and is performed at constant current until the voltage has rises to 90 to 100 volts, and thereafter it is continued at constant voltage.
Claim 18
Dependent Claim
17 words
Method as claimed in claim 1 wherein capillary gel slabs polymerized in the horizontal position are used.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-12-14 | 1985-06-15 | 1985-12-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-12-14 | 1989-06-15 | 1989-12-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-12-14 | 1993-06-15 | 1993-12-15 | $7700.00 | Window Closed / Lapsed |