Patent US3415425A successfully refreshed from Google Patents.
Patent US4384957A successfully refreshed from Google Patents.
US4384957A
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Molecular separation column and use thereof
Assignee: AMF INCORPORATED A CORP OF
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Filing: 1981-07-28
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Granted: 1983-05-24
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Expires: 2001-07-28
Abstract
Abstract A molecular separation chromotography column for effecting the differential distribution, between two phases, of the components of a sample flowing therethrough. The column contains a substantially homogeneous solid stationary phase which comprises a porous matrix of fiber having particulate immobilized therein, wherein at least one of said fiber or particulate is effective for molecular separation. The column is characterized by a reduced pressure drop, increased axial dispersion, more uniform peak shapes and better separations at high sample loading.
Inventors
- 👤 Alvin L. Crowder, III
- 👤 Nils L. Dailey
- 👤 Joseph V. Fiore
- 👤 Kenneth C. Hou
- 👤 Paul J. Marinaccio
- 👤 Eugene A. Ostreicher
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 07:31
CPC Classifications
B; B01; B01J; B01J20/00; B01J20/281; B01J20/282; B01D; B01D15/00; B01D15/08; B01D15/10; B01D15/22; B01J20/28; B01J20/28002; B01J20/28004; B01J20/28014; B01J20/28028; B01J20/28033; B01J20/28052; G; G01; G01N; G01N30/00; G01N30/02; G01N30/50; G01N30/56; B01J2220/00; B01J2220/50; B01J2220/54; G01N30/52; G01N2030/524; G01N2030/527
Claims Analysis
Total Claims: 32
Claim 1
Independent Claim
113 words
A chromatography column for effecting differential distribution, between two phases, of the components of a sample flowing therethrough, said column containing a solid stationary phase substantially homogeneous with respect to each component thereof, which comprises a porous matrix of fiber having particulate immobilized therein, at least one of said fiber or particulate therein having chromatographic functionality and being effective for chromatographic separation, wherein said solid stationary phase comprises a plurality of sheets of said solid stationary phase elements, the edges of said elements cooperating with the interior wall of the column to form a substantially fluid tight seal therewith, preventing any appreciable skewing or by-pass of fluid around the edges of the elements.
Claim 2
Dependent Claim
113 words
A chromatography column for effecting differential distribution, between two phases, of the components of a sample flowing therethrough, said column containing a solid stationary phase substantially homogeneous with respect to each component thereof, which comprises a porous matrix of fiber having particulate immobilized therein, at least one of said fiber or particulate therein having chromatographic functionality and being effective for chromatographic separation, wherein said solid stationary phase comprises a plurality of sheets of said solid stationary phase elements, the edges of said elements cooperating with the interior wall of the column to form a substantially fluid tight seal therewith, preventing any appreciable skewing or by-pass of fluid around the edges of the elements.
Claim 3
Dependent Claim
18 words
The chromatography column of claim 1, wherein the particulate has chromatographic functionality and is effective for chromatographic separation.
Claim 4
Dependent Claim
11 words
The chromatography column of claim 2, wherein the fibers are cellulose.
Claim 5
Dependent Claim
41 words
The chromatography column of claim 3, wherein the matrix comprises a major amount of long self bonding structural fiber and a minor amount of refined pulp fiber whose Canadian Standard Freeness is in the range of about +100 to -600 ml.
Claim 6
Dependent Claim
20 words
The chromatography column of claim 4, wherein the ratio of structural fiber to refined pulp fiber is 2:1 to 10:1.
Claim 7
Dependent Claim
13 words
The chromatography column of claim 5, wherein the ratio is 3:1 to 5:1.
Claim 8
Dependent Claim
28 words
The chromatography column of claims 1, 2, 3, 4, 5 or 6, wherein the amount of particulate is at least 10 weight percent of said solid stationary phase.
Claim 9
Dependent Claim
23 words
The chromatography column of claim 7, wherein the amount of particulate is from 10 to 80 weight percent of said solid stationary phase.
Claim 10
Dependent Claim
24 words
The chromatography column of claims 1, 2, 3, 4, 5 or 6, wherein said particulate has a mean particle size of about 5-100 microns.
Claim 11
Dependent Claim
22 words
The chromatography column of claim 4, wherein said structural fiber is cellulose having a Canadian Standard Freeness of +400 to +800 ml.
Claim 12
Dependent Claim
22 words
The chromatography column of claim 1, 2, 3, 4, 5 or 6, wherein said column is cylindrical and said sheets are discs.
Claim 13
Dependent Claim
14 words
The chromatography column of claim 11, wherein said solid stationary phase is hydrophilic swellable.
Claim 14
Dependent Claim
25 words
The chromatography column of claim 12, wherein said elements form a fluid-tight seal with the interior wall of the cylinder by water swellable fit therewith.
Claim 15
Dependent Claim
62 words
The chromatography column of claim 13, wherein said particulate has a mean particle size of from about 5 to 100 microns and is about 10 to 80 weight percent of said solid stationary phase, said structural fiber is cellulose having a Canadian Standard Freeness of +400 to +800 ml and a ratio of said structural fiber and refined pulp fiber is 3:1-5:1.
Claim 16
Dependent Claim
29 words
The chromatography column of claim 1, 2, 3 or 4, wherein said elements form a fluid-tight seal with the interior wall of the cylinder by compression friction fit therewith.
Claim 17
Dependent Claim
133 words
In a method of effecting a chromatographic separation by effecting a differential distribution of a sample's components between two phases by passing a mobile phase through a chromatography column containing a solid stationary phase, the improvement which comprises employing as said stationary phase a porous matrix of fiber having particulate immobilized therein, at least one of said fiber or particulate having chromatographic functionality and being effective for chromatographic separation, said solid stationary phase being substantially homogeneous with respect to each component thereof, wherein said solid stationary phase comprises a plurality of sheets of said solid stationary phase elements, the edges of said elements cooperating with the interior wall of the column to form a substantially fluid tight seal therewith, preventing any appreciable skewing or bypass of fluid around the edges of the elements.
Claim 18
Dependent Claim
133 words
In a method of effecting a chromatographic separation by effecting a differential distribution of a sample's components between two phases by passing a mobile phase through a chromatography column containing a solid stationary phase, the improvement which comprises employing as said stationary phase a porous matrix of fiber having particulate immobilized therein, at least one of said fiber or particulate having chromatographic functionality and being effective for chromatographic separation, said solid stationary phase being substantially homogeneous with respect to each component thereof, wherein said solid stationary phase comprises a plurality of sheets of said solid stationary phase elements, the edges of said elements cooperating with the interior wall of the column to form a substantially fluid tight seal therewith, preventing any appreciable skewing or bypass of fluid around the edges of the elements.
Claim 19
Dependent Claim
16 words
The method claim 16, wherein the particulate has chromatographic functionality and is effective for chromatographic separation.
Claim 20
Dependent Claim
11 words
The method of claim 16, wherein the mobile phase is liquid.
Claim 21
Dependent Claim
10 words
The method of claim 17, wherein the fibers are cellulose.
Claim 22
Dependent Claim
40 words
The method of claim 19, wherein the matrix comprises a major amount of long self bonding structural fiber and a minor amount of refined pulp fiber whose Canadian Standard Freeness is in the range of about +100 to -600 ml.
Claim 23
Dependent Claim
19 words
The method of claim 20, wherein the ratio of structural fiber to refined pulp fiber is 2:1 to 10:1.
Claim 24
Dependent Claim
12 words
The method of claim 21, wherein the ratio is 3:1 to 5:1.
Claim 25
Independent Claim
25 words
The method of claims 16, 17, 18, 19 or 20 wherein the amount of particulate is at least 10 percent of said solid stationary phase.
Claim 26
Dependent Claim
22 words
The method of claim 23, wherein the amount of particulate is from 10 to 80 weight percent of said solid stationary phase.
Claim 27
Independent Claim
24 words
The method of claims 16, 17, 18, 19 or 20, wherein said particulate has a mean particle size of about 5 to 100 microns.
Claim 28
Dependent Claim
21 words
The method of claim 20, wherein said structural fiber is cellulose having a Canadian Standard Freeness of +400 to +800 ml.
Claim 29
Dependent Claim
22 words
The method of claim 16, 17, 18, 19, 20, 21 or 22, wherein said column is cylindrical and said sheets are discs.
Claim 30
Dependent Claim
13 words
The method of claim 27, wherein said solid stationary phase is hydrophilic swellable.
Claim 31
Dependent Claim
24 words
The method of claim 28, wherein said elements form a fluid-tight seal with the interior wall of the cylinder by water swellable fit therewith.
Claim 32
Dependent Claim
61 words
The method of claim 29 wherein said particulate has a mean particle size of from about 5 to 100 microns and is about 10 to 80 weight percent of said solid stationary phase, said structural fiber is cellulose having a Canadian Standard Freeness of +400 to +800 ml and the ratio of said structural fiber and refined pulp fiber is 3:1-5:1.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-05-23 | 1986-11-22 | 1987-05-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-05-23 | 1990-11-22 | 1991-05-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-05-23 | 1994-11-22 | 1995-05-24 | $7700.00 | Window Closed / Lapsed |