US4384957A — Molecular separation column and use thereof

Claim 1

Independent Claim 113 words

A chromatography column for effecting differential distribution, between two phases, of the components of a sample flowing therethrough, said column containing a solid stationary phase substantially homogeneous with respect to each component thereof, which comprises a porous matrix of fiber having particulate immobilized therein, at least one of said fiber or particulate therein having chromatographic functionality and being effective for chromatographic separation, wherein said solid stationary phase comprises a plurality of sheets of said solid stationary phase elements, the edges of said elements cooperating with the interior wall of the column to form a substantially fluid tight seal therewith, preventing any appreciable skewing or by-pass of fluid around the edges of the elements.

Claim 2

Dependent Claim 113 words

A chromatography column for effecting differential distribution, between two phases, of the components of a sample flowing therethrough, said column containing a solid stationary phase substantially homogeneous with respect to each component thereof, which comprises a porous matrix of fiber having particulate immobilized therein, at least one of said fiber or particulate therein having chromatographic functionality and being effective for chromatographic separation, wherein said solid stationary phase comprises a plurality of sheets of said solid stationary phase elements, the edges of said elements cooperating with the interior wall of the column to form a substantially fluid tight seal therewith, preventing any appreciable skewing or by-pass of fluid around the edges of the elements.

Claim 3

Dependent Claim 18 words

The chromatography column of claim 1, wherein the particulate has chromatographic functionality and is effective for chromatographic separation.

Claim 4

Dependent Claim 11 words

The chromatography column of claim 2, wherein the fibers are cellulose.

Claim 5

Dependent Claim 41 words

The chromatography column of claim 3, wherein the matrix comprises a major amount of long self bonding structural fiber and a minor amount of refined pulp fiber whose Canadian Standard Freeness is in the range of about +100 to -600 ml.

Claim 6

Dependent Claim 20 words

The chromatography column of claim 4, wherein the ratio of structural fiber to refined pulp fiber is 2:1 to 10:1.

Claim 7

Dependent Claim 13 words

The chromatography column of claim 5, wherein the ratio is 3:1 to 5:1.

Claim 8

Dependent Claim 28 words

The chromatography column of claims 1, 2, 3, 4, 5 or 6, wherein the amount of particulate is at least 10 weight percent of said solid stationary phase.

Claim 9

Dependent Claim 23 words

The chromatography column of claim 7, wherein the amount of particulate is from 10 to 80 weight percent of said solid stationary phase.

Claim 10

Dependent Claim 24 words

The chromatography column of claims 1, 2, 3, 4, 5 or 6, wherein said particulate has a mean particle size of about 5-100 microns.

Claim 11

Dependent Claim 22 words

The chromatography column of claim 4, wherein said structural fiber is cellulose having a Canadian Standard Freeness of +400 to +800 ml.

Claim 12

Dependent Claim 22 words

The chromatography column of claim 1, 2, 3, 4, 5 or 6, wherein said column is cylindrical and said sheets are discs.

Claim 13

Dependent Claim 14 words

The chromatography column of claim 11, wherein said solid stationary phase is hydrophilic swellable.

Claim 14

Dependent Claim 25 words

The chromatography column of claim 12, wherein said elements form a fluid-tight seal with the interior wall of the cylinder by water swellable fit therewith.

Claim 15

Dependent Claim 62 words

The chromatography column of claim 13, wherein said particulate has a mean particle size of from about 5 to 100 microns and is about 10 to 80 weight percent of said solid stationary phase, said structural fiber is cellulose having a Canadian Standard Freeness of +400 to +800 ml and a ratio of said structural fiber and refined pulp fiber is 3:1-5:1.

Claim 16

Dependent Claim 29 words

The chromatography column of claim 1, 2, 3 or 4, wherein said elements form a fluid-tight seal with the interior wall of the cylinder by compression friction fit therewith.

Claim 17

Dependent Claim 133 words

In a method of effecting a chromatographic separation by effecting a differential distribution of a sample's components between two phases by passing a mobile phase through a chromatography column containing a solid stationary phase, the improvement which comprises employing as said stationary phase a porous matrix of fiber having particulate immobilized therein, at least one of said fiber or particulate having chromatographic functionality and being effective for chromatographic separation, said solid stationary phase being substantially homogeneous with respect to each component thereof, wherein said solid stationary phase comprises a plurality of sheets of said solid stationary phase elements, the edges of said elements cooperating with the interior wall of the column to form a substantially fluid tight seal therewith, preventing any appreciable skewing or bypass of fluid around the edges of the elements.

Claim 18

Dependent Claim 133 words

In a method of effecting a chromatographic separation by effecting a differential distribution of a sample's components between two phases by passing a mobile phase through a chromatography column containing a solid stationary phase, the improvement which comprises employing as said stationary phase a porous matrix of fiber having particulate immobilized therein, at least one of said fiber or particulate having chromatographic functionality and being effective for chromatographic separation, said solid stationary phase being substantially homogeneous with respect to each component thereof, wherein said solid stationary phase comprises a plurality of sheets of said solid stationary phase elements, the edges of said elements cooperating with the interior wall of the column to form a substantially fluid tight seal therewith, preventing any appreciable skewing or bypass of fluid around the edges of the elements.

Claim 19

Dependent Claim 16 words

The method claim 16, wherein the particulate has chromatographic functionality and is effective for chromatographic separation.

Claim 20

Dependent Claim 11 words

The method of claim 16, wherein the mobile phase is liquid.

Claim 21

Dependent Claim 10 words

The method of claim 17, wherein the fibers are cellulose.

Claim 22

Dependent Claim 40 words

The method of claim 19, wherein the matrix comprises a major amount of long self bonding structural fiber and a minor amount of refined pulp fiber whose Canadian Standard Freeness is in the range of about +100 to -600 ml.

Claim 23

Dependent Claim 19 words

The method of claim 20, wherein the ratio of structural fiber to refined pulp fiber is 2:1 to 10:1.

Claim 24

Dependent Claim 12 words

The method of claim 21, wherein the ratio is 3:1 to 5:1.

Claim 25

Independent Claim 25 words

The method of claims 16, 17, 18, 19 or 20 wherein the amount of particulate is at least 10 percent of said solid stationary phase.

Claim 26

Dependent Claim 22 words

The method of claim 23, wherein the amount of particulate is from 10 to 80 weight percent of said solid stationary phase.

Claim 27

Independent Claim 24 words

The method of claims 16, 17, 18, 19 or 20, wherein said particulate has a mean particle size of about 5 to 100 microns.

Claim 28

Dependent Claim 21 words

The method of claim 20, wherein said structural fiber is cellulose having a Canadian Standard Freeness of +400 to +800 ml.

Claim 29

Dependent Claim 22 words

The method of claim 16, 17, 18, 19, 20, 21 or 22, wherein said column is cylindrical and said sheets are discs.

Claim 30

Dependent Claim 13 words

The method of claim 27, wherein said solid stationary phase is hydrophilic swellable.

Claim 31

Dependent Claim 24 words

The method of claim 28, wherein said elements form a fluid-tight seal with the interior wall of the cylinder by water swellable fit therewith.

Claim 32

Dependent Claim 61 words

The method of claim 29 wherein said particulate has a mean particle size of from about 5 to 100 microns and is about 10 to 80 weight percent of said solid stationary phase, said structural fiber is cellulose having a Canadian Standard Freeness of +400 to +800 ml and the ratio of said structural fiber and refined pulp fiber is 3:1-5:1.

Annuity Stage	Window Opens	Payment Deadline	Surcharge Period Until	Estimated Fee	Status
3.5 Years (Year 4)	1986-05-23	1986-11-22	1987-05-24	$2000.00	Window Closed / Lapsed
7.5 Years (Year 8)	1990-05-23	1990-11-22	1991-05-24	$3760.00	Window Closed / Lapsed
11.5 Years (Year 12)	1994-05-23	1994-11-22	1995-05-24	$7700.00	Window Closed / Lapsed

Molecular separation column and use thereof

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