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US4383036A
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Process for the production of human chorionic gonadotropin
Assignee: Hayashibara Seibutsu Kagaku Kenkyujo KK
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Filing: 1981-08-07
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Granted: 1983-05-10
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Expires: 2001-08-07
Abstract
Abstract The present invention relates to a process for the production of human chorionic gonadotropin (hCG). More precisely, the invention relates to a process for the mass production of hCG, comprising in vivo multiplication of human lymphoblastoid cells capable of producing hCG, and hCG production by the multiplied human lymphoblastoid cells. The hCG production according to the invention is extremely higher, in terms of hCG production per cell, than that attained by conventional process using in vitro tissue culture; thus, hCG can be used in a sufficient amount in the prevention and treatment of human diseases.
Inventors
- 👤 Kaname Sugimoto
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 14:57
CPC Classifications
C; C12; C12N; C12N15/00; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/59; C12P; C12P21/00; A; A61; A61K; A61K38/00
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
174 words
A process for producing human chorionic gonadotropin (hCG), which comprises: (1) implanting human lymphoblastoid cells capable of producing hCG into a non-human warm-blooded animal; feeding the animal to cause the human cells therein to multiply; extracting and disaggregating the resultant multiplied human cells formed in the animal; culturing the human cells in a nutrient medium for a period sufficient to accumulate a significant amount of hCG; and harvesting the accumulated hCG from the culture, or alternatively, (2) placing human lymphoblastoid cells capable of producing hCG in suspension in a diffusion chamber; embedding the chamber in or placing the chamber on a non-human warm-blooded animal in a manner such that the nutrient body fluid of the non-human warm-blooded animal is supplied to the cells within the chamber; feeding the animal to cause the human cells in the chamber to multiply; collecting the multiplied human cells from the chamber; culturing the human cells in a nutrient medium for a period sufficient to accumulate a significant amount of hCG; and harvesting the accumulated hCG from the culture.
Claim 2
Dependent Claim
174 words
A process for producing human chorionic gonadotropin (hCG), which comprises: (1) implanting human lymphoblastoid cells capable of producing hCG into a non-human warm-blooded animal; feeding the animal to cause the human cells therein to multiply; extracting and disaggregating the resultant multiplied human cells formed in the animal; culturing the human cells in a nutrient medium for a period sufficient to accumulate a significant amount of hCG; and harvesting the accumulated hCG from the culture, or alternatively, (2) placing human lymphoblastoid cells capable of producing hCG in suspension in a diffusion chamber; embedding the chamber in or placing the chamber on a non-human warm-blooded animal in a manner such that the nutrient body fluid of the non-human warm-blooded animal is supplied to the cells within the chamber; feeding the animal to cause the human cells in the chamber to multiply; collecting the multiplied human cells from the chamber; culturing the human cells in a nutrient medium for a period sufficient to accumulate a significant amount of hCG; and harvesting the accumulated hCG from the culture.
Claim 3
Dependent Claim
37 words
A process as set forth in claim 1, wherein said human lymphoblastoid cells capable of producing hCG are hybridoma cells derived by cell fusion of an established human lymphoblastoid line with human cells capable of producing hCG.
Claim 4
Dependent Claim
32 words
A process as set forth in claim 2, wherein said human cells capable of producing hCG which are fused to the established human lymphoblastoid line are human syncytiotrophoblast cells of chorionic villi.
Claim 5
Dependent Claim
29 words
A process as set forth in claim 2, wherein said human cells capable of producing hCG which are fused to the established human lymphoblastoid line are human chorioepithelioma cells.
Claim 6
Dependent Claim
33 words
A process as set forth in claim 2, wherein said human cells capable of producing hCG which are fused to the established human lymphoblastoid line are human chromophobe cells of the pituitary gland.
Claim 7
Dependent Claim
19 words
A process as set forth in claim 1, wherein said non-human warm-blooded animal is a poultry or a mammalian.
Claim 8
Dependent Claim
34 words
A process as set forth in claim 1, wherein said culturing steps are carried out in the presence of one or more members of the group consisting of amino acids, inorganic salts, and hormones.
Claim 9
Dependent Claim
20 words
A process as set forth in claim 2, wherein said cell fusion is accomplished using Sendai virus or polyethylene glycol.
Claim 10
Dependent Claim
21 words
A process as set forth in claim 2, wherein said established human lymphoblastoid line is Namalwa, BALL-1, NALL-1, TALL-1, or JBL.
Claim 11
Dependent Claim
33 words
A process as set forth in claim 1, wherein said non-human warm-blooded animal is a chicken, pigeon, dog, cat, monkey, goat, pig, cow, horse, rabbit, guinea pig, rat, hamster, mouse or nude mouse.
Claim 12
Dependent Claim
46 words
A process as set forth in claim 1, wherein said culturing steps are carried out in the presence of one or more members selected from the group consisting of leucine, lysine, arginine, cysteine, sodium chloride, potassium chloride, calcium chloride, magnesium sulfate, and luteinizing hormone releasing hormone.
References Cited (Backward Citations)
US4288546A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-05-09 | 1986-11-08 | 1987-05-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-05-09 | 1990-11-08 | 1991-05-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-05-09 | 1994-11-08 | 1995-05-10 | $7700.00 | Window Closed / Lapsed |