Separation of plasma proteins from cell culture systems

Claim 2

A process of separating a plasma protein component from its admixture in a cell culture system comprising adsorbing said plasma protein component therefrom by contact with a water-insoluble, cross-linked polyelectrolyte copolymer of olefinically unsaturated monomer having from 2 to about 4 carbon atoms and α,β-unsaturated polycarboxylic acid or anhydride having from 4 to about 6 carbon atoms and containing pendant diloweralkylaminoloweralkylimide functional groups.

Claim 3

The process of claim 1 in which the cell culture system is a microbial cell culture system.

Claim 4

The process of claim 1 in which the cell culture system is a mammalian cell culture system.

Claim 5

The process of claim 2 in which the microbial cell culture system is a fermentation broth.

Claim 6

The process of claim 3 in which the mammalian cell culture system is an expended cell culture medium.

Claim 7

The process of claim 2 in which the microbial cell culture system is an isolate of disrupted microbial cells.

Claim 8

The process of claim 3 in which the mammalian cell culture system is an isolate of disrupted mammalian cells.

Claim 9

The process of claim 2 in which the microbial cells are bacteria cells.

Claim 10

The process of claim 8 in which the bacteria cells are Escherichia coli cells.

Claim 11

The process of claim 2 in which the microbial cells are yeast cells.

Claim 12

The process of claim 10 in which the yeast cells are Saccharomyces cerevisiae cells.

Claim 13

The process of claim 13 in which the mammalian cells are human liver cells.

Claim 14

The process of claim 12 in which the human liver cells are SK-HEP-1 cells.

Claim 15

The process of claim 1 in which the olefinically unsaturated monomer is ethylene and the α,β-unsaturated polycarboxylic acid or anhydride is maleic acid or anhydride.

Claim 16

The process of claim 14 in which the diloweralkylaminoloweralkylimide is dimethylaminopropylimide.

Claim 17

The method of claim 1 in which the plasma protein is human plasma Factor VIII.

Claim 18

The method of claim 1 in which the plasma protein is human serum albumin.

Claim 19

The method of claim 16 in which the olefinically unsaturated monomer is ethylene, the α,β-unsaturated polycarboxylic acid or anhydride is maleic acid or anhydride, the pendant diloweralkylaminoloweralkylimide is dimethylaminopropylimide and in which the polyelectrolyte copolymer contains from about 3 mole % to about 7 mole % of said pendant dimethylaminopropylimide and all the remaining free carboxyl or anhydride sites are blocked with alkoxyalkylamine wherein said alkyl and alkoxy have from one to about four carbon atoms.

Claim 20

The method of claim 17 in which the olefinically unsaturated monomer is ethylene, the α,β-unsaturated polycarboxylic acid or anhydride is maleic acid or anhydride, the pendant diloweralkylaminoloweralkylimide is dimethylaminopropylimide and in which the polyelectrolyte copolymer contains from about 90 mole % to about 100 mole % of said pendant dimethylaminopropylimide.