Patent US3729451A successfully refreshed from Google Patents.
US4157431A
Expired / Lapsed
US
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Separation of blood coagulation factors with non-activating polyelectrolytes
Assignee: Monsanto Co
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Filing: 1978-08-15
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Granted: 1979-06-05
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Expires: 1998-08-15
Abstract
Abstract {PG,1 Blood coagulation factors such as Factor VIII are separated from admixture with other blood proteins without producing activation of said coagulation factors by contacting with a water-insoluble, cross-linked polyelectrolyte copolymer of (a) C{HD 2{14 18 {L unsaturated monomer and (b) C{HD 4{14 12 {L unsaturated polycarboxylic acid or anhydride which is partially substituted at its free carboxyl or anhydride sites with amine-imides and in which substantially all the remaining free carboxyl or anhydride sites are blocked with alkoxyalkylamine to form alkoxyalkylimide units.
Inventors
- 👤 Joseph E. Fields
- 👤 Robert J. Slocombe
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 20:56
CPC Classifications
C; C08; C08L; C08L35/00; C07; C07K; C07K14/00; C07K14/435; C07K14/745; C07K14/755; C08F; C08F8/00; C08F8/44; A; A61; A61K; A61K38/00; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
127 words
A method for separating specific blood coagulation factor from admixture with other blood proteins in a fluid medium comprising the step of contacting said medium with a water-insoluble, cross-linked polyelectrolyte copolymer of (a) unsaturated monomer having from 2 to about 18 carbon atoms and (b) a monomer selected from the group consisting of unsaturated polycarboxylic acid or anhydride having from about 4 to about 12 carbon atoms which is partially substituted at its free carboxyl or anhydride sites with amine-imides and which is further characterized in that substantially all the remaining free carboxyl or anhydride sites are blocked with alkoxyalkylamine, to thereby selectively adsorb said specific coagulation factor to the substantial exclusion of said other blood proteins without producing any substantial preactivation of said specific coagulation factor.
Claim 2
Independent Claim
127 words
A method for separating specific blood coagulation factor from admixture with other blood proteins in a fluid medium comprising the step of contacting said medium with a water-insoluble, cross-linked polyelectrolyte copolymer of (a) unsaturated monomer having from 2 to about 18 carbon atoms and (b) a monomer selected from the group consisting of unsaturated polycarboxylic acid or anhydride having from about 4 to about 12 carbon atoms which is partially substituted at its free carboxyl or anhydride sites with amine-imides and which is further characterized in that substantially all the remaining free carboxyl or anhydride sites are blocked with alkoxyalkylamine, to thereby selectively adsorb said specific coagulation factor to the substantial exclusion of said other blood proteins without producing any substantial preactivation of said specific coagulation factor.
Claim 3
Dependent Claim
29 words
The method of claim 1 in which the copolymer is a copolymer of maleic acid or anhydride and monomer selected from the group consisting of ethylene, styrene and isobutylene.
Claim 4
Dependent Claim
19 words
The method of claim 2 in which the copolymer is a copolymer of ethylene and maleic acid or anhydride.
Claim 5
Dependent Claim
19 words
The method of claim 1 in which the amine-imide is selected from the group consisting of diloweralkylaminoloweralkylimide and loweralkyliminodi(loweralkylimide).
Claim 6
Dependent Claim
18 words
The method of claim 1 in which the copolymer is cross-linked with methyliminobispropylamine and the amine-imide is dimethylaminopropylimide.
Claim 7
Dependent Claim
19 words
The method of claim 1 in which the alkoxyalkylamine is selected from the group consisting of methoxyethylamine and methoxypropylamine.
Claim 8
Dependent Claim
26 words
The method of claim 1 in which the partial substitution with amine-imide ranges from about 2% to about 90% of the free carboxyl or anhydride sites.
Claim 9
Dependent Claim
33 words
The method of claim 1 in which the specific coagulation factor is Factor VIII and the contacting with the polyelectrolyte is carried out at a pH ranging from about 5.5 to about 9.5.
Claim 10
Dependent Claim
120 words
In the method of making a water-insoluble, cross-linked polyelectrolyte of the type suitable for the factionation of blood components and comprising a copolymer of (a) unsaturated monomer having from 2 to about 18 carbon atoms and (b) a monomer selected from the group consisting of unsaturated polycarboxylic acid or anhydride having from about 4 to about 12 carbon atoms which is partially substituted at its free carboxyl or anhydride sites with amine-imides, the improvement comprising blocking substantially all the remaining free carboxyl or anhydride sites with alkoxyalkylamine whereby said polyelectrolyte can be used for the separation of specific blood coagulation factor from other blood proteins in a fluid medium without thereby causing any substantial preactivation of said specific coagulation factor.
Claim 11
Dependent Claim
120 words
In the method of making a water-insoluble, cross-linked polyelectrolyte of the type suitable for the factionation of blood components and comprising a copolymer of (a) unsaturated monomer having from 2 to about 18 carbon atoms and (b) a monomer selected from the group consisting of unsaturated polycarboxylic acid or anhydride having from about 4 to about 12 carbon atoms which is partially substituted at its free carboxyl or anhydride sites with amine-imides, the improvement comprising blocking substantially all the remaining free carboxyl or anhydride sites with alkoxyalkylamine whereby said polyelectrolyte can be used for the separation of specific blood coagulation factor from other blood proteins in a fluid medium without thereby causing any substantial preactivation of said specific coagulation factor.
Claim 12
Dependent Claim
29 words
The method of claim 9 in which the copolymer is a copolymer of maleic acid or anhydride and monomer selected from the group consisting of ethylene, styrene and isobutylene.
Claim 13
Dependent Claim
19 words
The method of claim 10 in which the copolymer is a copolymer of ethylene and maleic acid or anhydride.
Claim 14
Dependent Claim
19 words
The method of claim 9 in which the amine-imide is selected from the group consisting of diloweralkylaminoloweralkylimide and loweralkyliminodi(loweralkylimide).
Claim 15
Dependent Claim
18 words
The method of claim 9 in which the copolymer is cross-linked with methyliminobispropylamine and the amine-imide is dimethylaminopropylimide.
Claim 16
Dependent Claim
19 words
The method of claim 9 in which the alkoxyalkylamine is selected from the group consisting of methoxyethylamine and methoxypropylamine.
Claim 17
Dependent Claim
26 words
The method of claim 9 in which the partial substitution with amine-imide ranges from about 2% to about 90% of the free carboxyl or anhydride sites.
Claim 18
Dependent Claim
119 words
A water-insoluble, cross-linked polyelectrolyte of the type suitable for the fractionation of blood components comprising a copolymer of (a) unsaturated monomer having from 2 to about 18 carbon atoms and (b) a monomer selected from the group consisting of unsaturated polycarboxylic acid or anhydride having from about 4 to about 12 carbon atoms which is partially substituted at its free carboxyl or anhydride sites with amine-imides, and which is further characterized in that substantially all the remaining free carboxyl or anhydride sites are blocked with alkoxyalkylamine whereby said polyelectrolyte can be used for the separation of specific blood coagulation factor from other blood proteins in a fluid medium without thereby causing any substantial pre-activation of said specific coagulation factor.
Claim 19
Dependent Claim
119 words
A water-insoluble, cross-linked polyelectrolyte of the type suitable for the fractionation of blood components comprising a copolymer of (a) unsaturated monomer having from 2 to about 18 carbon atoms and (b) a monomer selected from the group consisting of unsaturated polycarboxylic acid or anhydride having from about 4 to about 12 carbon atoms which is partially substituted at its free carboxyl or anhydride sites with amine-imides, and which is further characterized in that substantially all the remaining free carboxyl or anhydride sites are blocked with alkoxyalkylamine whereby said polyelectrolyte can be used for the separation of specific blood coagulation factor from other blood proteins in a fluid medium without thereby causing any substantial pre-activation of said specific coagulation factor.
Claim 20
Dependent Claim
29 words
The polyelectrolyte of claim 16 in which the copolymer is a copolymer of maleic acid or anhydride and monomer selected from the group consisting of ethylene, styrene and isobutylene.
Claim 21
Dependent Claim
19 words
The polyelectrolyte of claim 17 in which the copolymer is a copolymer of ethylene and maleic acid or anhydride.
Claim 22
Dependent Claim
19 words
The polyelectrolyte of claim 16 in which the amine-imide is selected from the group consisting of diloweralkylaminoloweralkylimide and loweralkyliminodi(loweralkylimide).
Claim 23
Dependent Claim
18 words
The polyelectrolyte of claim 16 in which the copolymer is cross-linked with methyliminobispropylamine and the amine-imide is dimethylaminopropylimide.
Claim 24
Dependent Claim
19 words
The polyelectrolyte of claim 16 in which the alkoxyalkylamine is selected from the group consisting of methoxyethylamine and methoxypropylamine.
Claim 25
Dependent Claim
26 words
The polyelectrolyte of claim 16 in which the partial substitution with amine-imide ranges from about 2% to about 90% of the free carboxyl or anhydride sites.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-06-04 | 1982-12-04 | 1983-06-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-06-04 | 1986-12-04 | 1987-06-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-06-04 | 1990-12-04 | 1991-06-05 | $7700.00 | Window Closed / Lapsed |