Patent US4373931A successfully refreshed from Google Patents.
US4373931A
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Method of measuring agglutinating reaction and a reaction vessel therefor
Assignee: Olympus Corp
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Filing: 1980-10-03
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Granted: 1983-02-15
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Expires: 2000-10-03
Abstract
Abstract Method for measuring agglutinating reaction by checking an agglutinates pattern caused by immunological agglutinating reaction while using a reaction vessel having at least one smooth recess formed on an inner sidewall surface thereof. After reaction liquid is placed therein, the reaction vessel is rotated about an axis thereof, so as to accelerate the agglutinating reaction by collecting corpuscles of the liquid in the recess. If the agglutinating reaction occurs, agglutinates released from the recess quickly sediment on the bottom of the reaction vessel to form an agglutinates pattern to be detected.
Inventors
- 👤 Hiroshi Takekawa
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 15:51
CPC Classifications
B; B01; B01L; B01L3/00; B01L3/50; B01L3/502; B01L3/5021; B04; B04B; B04B5/00; B04B5/04; B04B5/0407; B04B7/00; B04B7/08; G; G01; G01N; G01N21/00; G01N21/01; G01N21/03; G01N21/07; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/5302; G01N33/5304
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
170 words
In a method of measuring an agglutination reaction by detecting an agglutination pattern due to an immunological agglutination reaction, the improvement comprising the steps of introducing a reaction liquid containing corpuscles into a reaction vessel having a bottom and at least one recess formed in an inner sidewall thereof and smoothly communicated with the bottom; rotating said reaction vessel about an axis thereof to collect at least a part of the reaction liquid in said recess for accelerating the agglutination reaction; stopping the rotation of the reaction vessel to release the reaction liquid including agglutinated or non-agglutinated corpuscles from said recess into the bottom of the vessel due to gravity; mixing the corpuscle-containing liquid with the reaction liquid contained in the bottom of the vessel so that, in case of agglutination, the agglutinated corpuscles settle on the bottom without being separated from each other, whereas in case of non-agglutination, the corpuscles are suspended in the reaction liquid; and detecting the agglutination pattern formed on the bottom by the settled corpuscles.
Claim 2
Dependent Claim
170 words
In a method of measuring an agglutination reaction by detecting an agglutination pattern due to an immunological agglutination reaction, the improvement comprising the steps of introducing a reaction liquid containing corpuscles into a reaction vessel having a bottom and at least one recess formed in an inner sidewall thereof and smoothly communicated with the bottom; rotating said reaction vessel about an axis thereof to collect at least a part of the reaction liquid in said recess for accelerating the agglutination reaction; stopping the rotation of the reaction vessel to release the reaction liquid including agglutinated or non-agglutinated corpuscles from said recess into the bottom of the vessel due to gravity; mixing the corpuscle-containing liquid with the reaction liquid contained in the bottom of the vessel so that, in case of agglutination, the agglutinated corpuscles settle on the bottom without being separated from each other, whereas in case of non-agglutination, the corpuscles are suspended in the reaction liquid; and detecting the agglutination pattern formed on the bottom by the settled corpuscles.
Claim 3
Dependent Claim
36 words
The method of claim 1, wherein a plurality of different reaction liquids are placed in corresponding reaction vessels and the reaction vessels are rotated simultaneously, each of said reaction vessels rotating about an axis passing therethrough.
Claim 4
Dependent Claim
15 words
The method of claim 1, wherein said reaction vessel has a substantially semi-spherical bottom surface.
Claim 5
Dependent Claim
12 words
The method of claim 1, wherein said recess is substantially semi-sphericallly shaped.
Claim 6
Dependent Claim
15 words
The method of claim 1, wherein said reaction vessel has a substantially conical bottom surface.
Claim 7
Dependent Claim
16 words
The method of claim 1, wherein said recess is substantially conically shaped with a rounded vertex.
Claim 8
Dependent Claim
20 words
The method of claim 1, wherein said reaction vessel has a permanent magnetic ring embedded at the bottom portion thereof.
Claim 9
Dependent Claim
107 words
A reaction vessel for measuring an agglutination reaction comprising: a substantially tubular member comprising a cylindrical body having a side wall and a longitudinal axis; an opening provided at an upper end of the cylindrical body; a bottom wall having a generally concave inner bottom surface; and a recess formed in said side wall, said recess having an inner surface which extends from the inner surface of the side wall of the cylindrical body, said recess having a central axis which is substantially perpendicular to the longitudinal axis of the cylindrical body for facilitating gravity flow of fluid from said recess back to said inner bottom surface.
Claim 10
Dependent Claim
107 words
A reaction vessel for measuring an agglutination reaction comprising: a substantially tubular member comprising a cylindrical body having a side wall and a longitudinal axis; an opening provided at an upper end of the cylindrical body; a bottom wall having a generally concave inner bottom surface; and a recess formed in said side wall, said recess having an inner surface which extends from the inner surface of the side wall of the cylindrical body, said recess having a central axis which is substantially perpendicular to the longitudinal axis of the cylindrical body for facilitating gravity flow of fluid from said recess back to said inner bottom surface.
Claim 11
Dependent Claim
19 words
A reaction vessel as set forth in claim 8, wherein said reaction vessel has a substantially semi-spherical bottom surface.
Claim 12
Dependent Claim
16 words
A reaction vessel as set forth in claim 8, wherein said recess is substantially semi-spherically shaped.
Claim 13
Dependent Claim
19 words
A reaction vessel as set forth in claim 8, wherein said reaction vessel has a substantially conical bottom surface.
Claim 14
Dependent Claim
20 words
A reaction vessel as set forth in claim 8, wherein said recess is substantially conically shaped with a rounded vertex.
Claim 15
Dependent Claim
24 words
A reaction vessel as set forth in claim 8, wherein said reaction vessel has a permanent magnetic ring embedded at the bottom portion thereof.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-02-14 | 1986-08-16 | 1987-02-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-02-14 | 1990-08-16 | 1991-02-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-02-14 | 1994-08-16 | 1995-02-15 | $7700.00 | Window Closed / Lapsed |