Patent US4371615A successfully refreshed from Google Patents.
US4371615A
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US
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Method for stabilizing the characteristics of microorganism containing a plasmid
Assignee: Ajinomoto Co Inc
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Filing: 1980-05-23
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Granted: 1983-02-01
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Expires: 2000-05-23
Abstract
Abstract A streptomycin dependent mutant of a microorganism of the genus Escherichia which contains a plasmid containing genetic information controlling streptomycin independence maintains its properties when cultured in a medium devoid of streptomycin. The plasmid may also contain genetic information controlling the production of a chemical compound by the microorganism. Fermentation cultures of such microorganisms in media devoid of streptomycin do not lose their industrially desirable ability to synthesize useful compounds.
Inventors
- 👤 Kiyoshi Miwa
- 👤 Haruo Momose
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 17:09
CPC Classifications
C; C12; C12P; C12P13/00; C12P13/04; C12P13/08; C12N; C12N1/00; C12N1/20; C12N1/205; C12N15/00; C12N15/09; C12N15/11; C12N15/52; C12N15/63; C12N15/67; C12R; C12R2001/00; C12R2001/01; C12R2001/185; C12R2001/19; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/848; Y10S435/849
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
73 words
A method for stabilizing the characteristics of a microorganism containing a plasmid, which comprises: introducing a plasmid obtained from a microorganism of the genus Escherichia into a mutant of the genus Escherichia, said plasmid having incorporated therein a chromosomal DNA fragment expressing independence of streptomycin, and the growth of said mutant being dependent on streptomycin; and cultivating and maintaining the stability of said plasmid containing microorganism in a growth medium free of streptomycin.
Claim 2
Independent Claim
73 words
A method for stabilizing the characteristics of a microorganism containing a plasmid, which comprises: introducing a plasmid obtained from a microorganism of the genus Escherichia into a mutant of the genus Escherichia, said plasmid having incorporated therein a chromosomal DNA fragment expressing independence of streptomycin, and the growth of said mutant being dependent on streptomycin; and cultivating and maintaining the stability of said plasmid containing microorganism in a growth medium free of streptomycin.
Claim 3
Dependent Claim
14 words
The method of claim 1, wherein said mutant belongs to the species Escherichia coli.
Claim 4
Dependent Claim
14 words
The method of claim 1, wherein said microorganism belongs to the species Escherichia coli.
Claim 5
Dependent Claim
11 words
The method of claim 1, wherein said plasmid is pBR 322.
Claim 6
Dependent Claim
22 words
The method of claim 1, wherein said plasmid further contains genetic information controlling the production of a chemical compound by said microorganism.
Claim 7
Dependent Claim
10 words
The method of claim 5, wherein said compound is threonine.
Claim 8
Dependent Claim
25 words
The method of claim 5, wherein said genetic information is in the form of a chromosomal fragment obtained from a microorganism of the genus Escherichia.
Claim 9
Independent Claim
81 words
A method for stabilizing the characteristics of a microorganism containing a plasmid, which comprises: introducing a plasmid obtained from a microorganism of the genus Escherichia into a mutant of the genus Escherichia, said plasmid having incorporated therein a chromosomal DNA fragment expressing independence of streptomycin and a chromosomal DNA fragment expressing threonine production, and the growth of said mutant being dependent on streptomycin; and cultivating and maintaining the stability of said plasmid containing microorganism in a growth medium free of streptomycin.
Claim 10
Independent Claim
81 words
A method for stabilizing the characteristics of a microorganism containing a plasmid, which comprises: introducing a plasmid obtained from a microorganism of the genus Escherichia into a mutant of the genus Escherichia, said plasmid having incorporated therein a chromosomal DNA fragment expressing independence of streptomycin and a chromosomal DNA fragment expressing threonine production, and the growth of said mutant being dependent on streptomycin; and cultivating and maintaining the stability of said plasmid containing microorganism in a growth medium free of streptomycin.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-01-31 | 1986-08-02 | 1987-02-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-01-31 | 1990-08-02 | 1991-02-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-01-31 | 1994-08-02 | 1995-02-01 | $7700.00 | Window Closed / Lapsed |