US4366250A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Preparation process of optically active α-aminated acids by biological hydrolysis of nitriles
Assignee: Bpifrance Financement SA
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Filing: 1980-01-24
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Granted: 1982-12-28
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Expires: 2000-01-24
Abstract
Abstract This process for preparing L α-amino-acid is characterized in that the corresponding racemic α-amino-acid is hydrolysed in liquid phase in a free form or in the form of a salt, by an agent containing an L-stereospecific amidase and in that the L α-amino-acid obtained is separated from the D α-amino-amid unhydrolysed. This process is appropriate for the preparation of optically active natural amino-acids.
Inventors
- 👤 Jean C. Jallageas
- 👤 Alain Arnaud
- 👤 Pierre Galzy
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 18:30
CPC Classifications
C; C12; C12P; C12P13/00; C12P13/04; C12N; C12N9/00; C12N9/14; C12N9/78; C12N9/80
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
58 words
A process for the preparation of L-alpha-amino acid from the corresponding racemic alpha-amino-nitrile in free form or as salt comprising hydrolizing in a single step in liquid medium racemic alpha-amino-nitrile in free form or as a salt with a bacteria having a general nitrilase and a L-stereospecific amidase and isolating the L-alpha amino acid from the D-alpha-amino amide.
Claim 2
Dependent Claim
58 words
A process for the preparation of L-alpha-amino acid from the corresponding racemic alpha-amino-nitrile in free form or as salt comprising hydrolizing in a single step in liquid medium racemic alpha-amino-nitrile in free form or as a salt with a bacteria having a general nitrilase and a L-stereospecific amidase and isolating the L-alpha amino acid from the D-alpha-amino amide.
Claim 3
Dependent Claim
41 words
Process pursuant to claim 1 wherein said bacteria is derived from a strain or mutant of a strain which possesses a L-sterospecific amidase, said strain being strain A 4 deposited under No. LMD 79.2, which is free from a general amidase.
Claim 4
Dependent Claim
22 words
Process pursuant to claim 2, characterized by the fact, that the hydrolysis is conducted at a pH, between 6 and 9 inclusive.
Claim 5
Dependent Claim
17 words
Process pursuant to claim 3 characterized by the fact, that said liquid medium is an aqueous medium.
Claim 6
Dependent Claim
49 words
A process pursuant to claim 1 wherein said bacteria is derived from a strain or mutant of a strain which possesses a general amidase, the process including the steps of growing said bacteria on a medium containing monofluoro-acetamide and selecting mutants with a general nitrilase and a L-stereospecific amidase.
Claim 7
Dependent Claim
27 words
Process pursuant to claim 5, characterized by the fact, that the strain, which possess a general amidase, are chosen from the types Bacillus, Bacteridium, Micrococcus and Brevibacterium.
Claim 8
Dependent Claim
73 words
Process pursuant to claim 5, characterized by the fact, that the strains, which possess a general amidase are chosen among the strain No. R 332, R 340, R 341, A 111, B 222, A 112, A 13, A 141, A 142, B 211, B 212, B 221, C 211, R 21, R 22, R 311, R 312, R 331, deposited at the Professorship for Genetics of the National Superior Agronomic School of Montpellier.
References Cited (Backward Citations)
US2511867A
Cited
US3635795A
Cited
FR2245585B1
Cited
US3971700A
Cited
FR2294999B1
Cited
FR2337761A1
Cited
US4080259A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-12-27 | 1986-06-28 | 1986-12-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-12-27 | 1990-06-28 | 1990-12-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-12-27 | 1994-06-28 | 1994-12-28 | $7700.00 | Window Closed / Lapsed |