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US4359463A
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Stabilization of Factor VIII activity in whole blood or blood plasma
Assignee: Canadian Red Cross Society
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Filing: 1980-11-26
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Granted: 1982-11-16
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Expires: 2000-11-26
Abstract
Abstract A method whereby initial Factor VIII activity normally present in blood collected into an anticoagulant which functions by chelating calcium is maintained for twenty-four or more hours without the necessity of the usual immediate freezing in either the whole blood or blood plasma. The method comprises mixing freshly collected blood or blood plasma prepared from that blood or freshly obtained blood plasma obtained by plasmapheresis, which blood or blood plasma has been collected into a calcium chelating anticoagulant, with a calcium-heparin solution in sufficient quantities to restore calcium to substantially normal physiological levels.
Inventors
- 👤 Gail A. Rock
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 15:33
CPC Classifications
A; A61; A61K; A61K31/00; A61K31/70; A61K31/715; A01; A01N; A01N1/00; A01N1/10; A01N1/12; A01N1/122; A01N1/126; A61K31/13; A61K31/14; A61K35/00; A61K35/12; A61K35/14; A61K35/16; A61K38/00; A61K38/16; A61K38/17; A61K38/36; A61K38/37; A61L; A61L2/00; A61L2/02; A61L2/04; A61L2103/00; A61L2103/05; A61L2103/09; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
69 words
A method for maintaining Factor VIII activity in blood or blood plasma comprising: collecting freshly obtained whole blood or blood plasma prepared from that blood or freshly obtained blood plasma prepared by plasmapheresis into a calcium chelating anticoagulant; and then mixing the collected product with a calcium-heparin solution, said calcium being added in sufficient quantity to restore calcium in said blood or blood plasma to substantially normal physiological levels.
Claim 2
Independent Claim
69 words
A method for maintaining Factor VIII activity in blood or blood plasma comprising: collecting freshly obtained whole blood or blood plasma prepared from that blood or freshly obtained blood plasma prepared by plasmapheresis into a calcium chelating anticoagulant; and then mixing the collected product with a calcium-heparin solution, said calcium being added in sufficient quantity to restore calcium in said blood or blood plasma to substantially normal physiological levels.
Claim 3
Dependent Claim
22 words
A method as claimed in claim 1 wherein the heparin-calcium solution is added immediately following collection of the blood into the anticoagulant.
Claim 4
Dependent Claim
23 words
A method as claimed in claim 1 wherein the heparin-calcium solution is added immediately to the plasma obtained from the freshly collected blood.
Claim 5
Dependent Claim
20 words
A method as claimed in claim 1 wherein the heparin-calcium solution is added immediately to the plasma obtained by plasmapheresis.
Claim 6
Dependent Claim
30 words
A method as claimed in claim 1 wherein the heparin-calcium solution is added at a time not more than six hours subsequent to collection or obtaining the blood or plasma.
Claim 7
Independent Claim
19 words
A method as claimed in any one of claims 2-5 or 1 wherein the anticoagulant is a citrate anticoagulant.
Claim 8
Dependent Claim
18 words
A method as claimed in claim 6 wherein the anticoagulant is citrate phosphate dextrose or acid citrate dextrose.
Claim 9
Independent Claim
28 words
A method as claimed in any one of claims 2-5 or 1 wherein the blood or plasma is mixed with 4-8 units per ml of a heparin solution.
Claim 10
Dependent Claim
16 words
A method as claimed in claim 8 wherein the heparin solution is a sodium heparin solution.
Claim 11
Dependent Claim
17 words
A method as claimed in claim 1 wherein the calcium is added in any soluble salt form.
Claim 12
Dependent Claim
32 words
A method as claimed in claim 1 or 10 wherein the calcium is added in an amount sufficient to return the level of calcium in the blood to approximately 5 mg %.
Claim 13
Dependent Claim
49 words
A method as claimed in claim 7 wherein the blood or plasma is mixed with 4-8 units per ml of sodium heparin solution and the calcium is a soluble salt added in an amount sufficient to return the level of calcium in the blood to approximately 5 mg %.
References Cited (Backward Citations)
US4203891A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-11-15 | 1986-05-17 | 1986-11-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-11-15 | 1990-05-17 | 1990-11-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-11-15 | 1994-05-17 | 1994-11-16 | $7700.00 | Window Closed / Lapsed |