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US4358288A
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US
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Fertility indicator system containing anthocyanin pigment
Assignee: Individual
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Filing: 1981-09-16
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Granted: 1982-11-09
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Expires: 2001-09-16
Abstract
Abstract Fertility in females, especially human females, is evaluated by contacting a mucin-containing body fluid such as saliva with a fertility evaluation medium comprising an anthocyanin pigment and a substrate which facilitates generation of a color response in the pigment, in the presence of vaginal fluid or substances similar to those found in the vaginal fluid, especially ascorbic acid and sugars. A resulting color change in the fertility evaluation medium can be used to determine whether the female is not fertile at that time, whether fertility is imminent in the female, or whether the female actually is fertile.
Inventors
- 👤 Dorothee F. E. Goldman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 17:59
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; Y; Y10; Y10S; Y10S436/00; Y10S436/906
Claims Analysis
Total Claims: 33
Claim 1
Independent Claim
98 words
Method for evaluating fertility in females, said method comprising: (a) providing a fertility evaluation medium comprising an anthocyanin pigment and a substrate for facilitating the generation of a color response in the pigment; (b) contacting a mucin-containing body fluid of a female with said fertility evaluation medium in the presence of vaginal fluid or substances found in vaginal fluid, including ascorbic acid and a sugar; and (c) determining from a resulting color change in said fertility evaluation medium whether said female is infertile, or whether fertility in said female is imminent, or whether said female is actually fertile.
Claim 2
Dependent Claim
98 words
Method for evaluating fertility in females, said method comprising: (a) providing a fertility evaluation medium comprising an anthocyanin pigment and a substrate for facilitating the generation of a color response in the pigment; (b) contacting a mucin-containing body fluid of a female with said fertility evaluation medium in the presence of vaginal fluid or substances found in vaginal fluid, including ascorbic acid and a sugar; and (c) determining from a resulting color change in said fertility evaluation medium whether said female is infertile, or whether fertility in said female is imminent, or whether said female is actually fertile.
Claim 3
Dependent Claim
98 words
Method according to claim 1, wherein said anthocyanin pigment is of the general formula: ##STR2## wherein R is selected from the group consisting of hydrogen, hydroxy, and C 1 -C 4 alkoxy; R' is selected from the group consisting of hydrogen, hydroxy, and C 1 -C 4 alkoxy; OR 3 and OR 5 are glycosides selected from the group consisting of glucosides, rutinosides, arabinosides, sophorosides, p-coumaroyl rutinosides and rhamnosides; n 1 and n 2 are each 0 or 1, provided that the sum of n 1 and n 2 is at least 1, and X' is an anion.
Claim 4
Dependent Claim
27 words
Method according to claim 1 wherein said mucin-containing body fluid is contacted with said fertility evaluation medium at a temperature of from 40° F. to 99° F.
Claim 5
Dependent Claim
14 words
Method according to claim 3 wherein said temperature is 40° F. to 90° F.
Claim 6
Dependent Claim
19 words
Method according to claim 1 wherein said fertility evaluation medium is maintained at pH of from 4 to 9.
Claim 7
Dependent Claim
12 words
Method according to claim 5 wherein said pH is 4.0 to 6.5.
Claim 8
Dependent Claim
12 words
Method according to claim 1 wherein said mucin-containing body fluid is saliva.
Claim 9
Dependent Claim
24 words
Method according to claim 1 wherein said substrate is a cellulose material selected from the group consisting of paper, cotton, hemp and powdered cellulose.
Claim 10
Dependent Claim
12 words
Method according to claim 1 wherein the female is a human female.
Claim 11
Dependent Claim
23 words
Method according to claim 1 wherein said sugar is selected from the group consisting of glucose, fructose, sucrose, galactose, sorbose, arabinose and dextrose.
Claim 12
Dependent Claim
22 words
Method according to claim 1 wherein said ascorbic acid is present in a concentration of from about 0.1 to 10% by weight.
Claim 13
Dependent Claim
18 words
Method according to claim 1 wherein said sugar is present in a concentration of about 1% by weight.
Claim 14
Dependent Claim
21 words
Method according to claim 1 wherein said anthocyanin pigment is derived from red cabbage, and includes cyanidin-3-p-coumaroyl sophoroside-5-glucoside, cyanidin-3-feruloylsophoroside-5-glucoside and cyanidin-3-diferuloylsophoroside-5-glucoside.
Claim 15
Dependent Claim
11 words
Method according to claim 1 wherein said anthocyanin pigment is cyanidin-3,5-diglucoside.
Claim 16
Dependent Claim
11 words
Method according to claim 1 wherein said anthocyanin pigment is delphinidin-3-glucoside.
Claim 17
Dependent Claim
53 words
Method according to claim 1 wherein said fertility evaluation medium comprises filter paper which has been impregnated with an anthocyanin pigment by applying to said filter paper a aqueous solution of said anthocyanin pigment in which said anthocyanin pigment is present in a concentration of from about 0.01 mg/ml to about 1 mg/ml.
Claim 18
Dependent Claim
22 words
Method according to claim 16 wherein said anthocyanin pigment is cyanidin, and the concentration of said aqueous solution is about 1 mg/ml.
Claim 19
Dependent Claim
22 words
Method according to claim 16 wherein said anthocyanin pigment is delphinidin and the concentration of said aqueous solution is about 0.01 mg/ml.
Claim 20
Dependent Claim
22 words
Method according to claim 16 wherein said anthocyanin pigment is petunidin, and the concentration of said aqueous solution is about 0.1 mg/ml.
Claim 21
Dependent Claim
23 words
Method according to claim 1 wherein said fertility evaluation medium comprises paper which has been impregnated with anthocyanin pigments present in red cabbage.
Claim 22
Dependent Claim
27 words
Method according to claim 1 wherein said substrate is selected from paper, cotton, and hemp, and said substrate is contacted with natural fruit source of ascorbic acid.
Claim 23
Dependent Claim
27 words
Method according to claim 21 wherein said natural fruit source of ascorbic acid is selected from the group consisting of lemon juice, tomato juice, and orange juice.
Claim 24
Dependent Claim
43 words
A composition suitable for use in evaluating fertility in females, said composition comprising an anthocyanin pigment, a substrate for facilitating generation of a color response in said pigment, and vaginal fluid or substances found in vaginal fluid, including ascorbic acid and a sugar.
Claim 25
Dependent Claim
43 words
A composition suitable for use in evaluating fertility in females, said composition comprising an anthocyanin pigment, a substrate for facilitating generation of a color response in said pigment, and vaginal fluid or substances found in vaginal fluid, including ascorbic acid and a sugar.
Claim 26
Dependent Claim
23 words
A composition according to claim 23 wherein said sugar is selected from the group consisting glucose, fructose, sucrose, galactose, sorbose, arabinose and dextrose.
Claim 27
Dependent Claim
25 words
A composition according to claim 23 wherein said substrate is a cellulose material selected from the group consisting of paper, cotton, hemp and powdered cellulose.
Claim 28
Dependent Claim
38 words
A composition according to claim 25 wherein said anthocyanin pigment is present in an amount of from about 0.01 mg/ml to about 1 mg/ml, and said cellulose substrate is present in an amount of about 10% by weight.
Claim 29
Dependent Claim
33 words
A composition according to claim 23 wherein said ascorbic acid is present in an amount of about 1% by weight and said sugar is present in an amount of about 1% by weight.
Claim 30
Dependent Claim
21 words
A composition according to claim 25 wherein said cellulose material is filter paper which has been impregnated with said anthocyanin pigment.
Claim 31
Dependent Claim
20 words
A composition according to claim 28 wherein said ascorbic acid is present in a natural fruit source of ascorbic acid.
Claim 32
Dependent Claim
88 words
A fertility evaluation kit suitable for use in the evaluation of fertility in females, said kit including a first, second and third component, said first component comprising an anthocyanin pigment and a substrate for facilitating the generation of a color response in said pigment, said second component comprising ascorbic acid and a sugar selected from the group consisting of glucose, fructose, sucrose, galactose, sorbose, arabinose and dextrose, said third component comprising a color comparison chart for comparing colors produced on said substrate with color displayed on the chart.
Claim 33
Dependent Claim
88 words
A fertility evaluation kit suitable for use in the evaluation of fertility in females, said kit including a first, second and third component, said first component comprising an anthocyanin pigment and a substrate for facilitating the generation of a color response in said pigment, said second component comprising ascorbic acid and a sugar selected from the group consisting of glucose, fructose, sucrose, galactose, sorbose, arabinose and dextrose, said third component comprising a color comparison chart for comparing colors produced on said substrate with color displayed on the chart.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-11-08 | 1986-05-10 | 1986-11-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-11-08 | 1990-05-10 | 1990-11-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-11-08 | 1994-05-10 | 1994-11-09 | $7700.00 | Window Closed / Lapsed |