Patent US4357323A successfully refreshed from Google Patents.
US4357323A
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Polysaccharide RBS substance and antitumor agent containing same
Assignee: Daicel Corp
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Filing: 1980-08-01
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Granted: 1982-11-02
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Expires: 2000-08-01
Abstract
Abstract A polysaccharide RBS substance having the ultraviolet absorption spectrum depicted in FIG. 1 and the infrared absorption spectrum depicited in FIG. 2 is obtained from rice bran by extraction with hot water. These substances have been tested for anti-tumor activity against Sarcoma 180 ascites tumor and against Ehrlich ascites tumor and were determined to have anti-tumor activity against these tested tumors.
Inventors
- 👤 Eichi Soma
- 👤 Kohei Kobayashi
- 👤 Takuro Karakawa
- 👤 Shigeyoshi Kato
- 👤 Kiichi Uchida
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 18:12
CPC Classifications
C; C08; C08B; C08B37/00; C08B37/0006; C08B37/0009; A; A61; A61P; A61P35/00
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
160 words
A polysaccharide RBS substance and pharmaceutically acceptable addition salts thereof (1) having the following properties: it does not pass through a semipermeable membrane; it is insoluble in alcohol, acetone, hexane, benzene, ethyl acetate, dimethylsulfoxide, ligroin, carbon tetrachloride, chloroform and ether, and is soluble or highly soluble in water; a 1% aqueous solution or suspension is neutral or slightly acidic; it reacts positively in the following reactions: the Molisch reaction, the anthrone-sulfuric acid reaction, the tryptophane-sulfuric acid reaction, the crystein-sulfuric acid reaction, the chromotrope-sulfuric acid reaction, the phenol-sulfuric acid reaction, the carbazole-sulfuric acid reaction, the biuret reaction, the ninhydrin reaction and the Lowry-Folin reaction; and negatively in the Elson-Morgan reaction and the starch-iodine reaction; it has the ultraviolet absorption spectrum depicted in FIG. 1 and the infrared absorption spectrum depicted in FIG. 2; and (2) containing glucose units as the sugar component, said glucose units being bonded by α-1,6 glucoside bonds as the main chain and said main chain is branched.
Claim 2
Dependent Claim
160 words
A polysaccharide RBS substance and pharmaceutically acceptable addition salts thereof (1) having the following properties: it does not pass through a semipermeable membrane; it is insoluble in alcohol, acetone, hexane, benzene, ethyl acetate, dimethylsulfoxide, ligroin, carbon tetrachloride, chloroform and ether, and is soluble or highly soluble in water; a 1% aqueous solution or suspension is neutral or slightly acidic; it reacts positively in the following reactions: the Molisch reaction, the anthrone-sulfuric acid reaction, the tryptophane-sulfuric acid reaction, the crystein-sulfuric acid reaction, the chromotrope-sulfuric acid reaction, the phenol-sulfuric acid reaction, the carbazole-sulfuric acid reaction, the biuret reaction, the ninhydrin reaction and the Lowry-Folin reaction; and negatively in the Elson-Morgan reaction and the starch-iodine reaction; it has the ultraviolet absorption spectrum depicted in FIG. 1 and the infrared absorption spectrum depicted in FIG. 2; and (2) containing glucose units as the sugar component, said glucose units being bonded by α-1,6 glucoside bonds as the main chain and said main chain is branched.
Claim 3
Dependent Claim
62 words
A pharmaceutical composition effective in inhibiting the growth of Sarcoma 180 ascites tumors, Erlich ascites tumors, and tumor cells (SV 40-transformed C3H-2K cells) consisting essentially of polysaccharide RBS substance and pharmaceutically acceptable acid-addition salts thereof in a pharmaceutically effective amount, and a pharmaceutically acceptable vehicle, said polysaccharide RBS substance (1) having the following properties: it does not pass through a semipermeable membrane;
Claim 4
Dependent Claim
62 words
A pharmaceutical composition effective in inhibiting the growth of Sarcoma 180 ascites tumors, Erlich ascites tumors, and tumor cells (SV 40-transformed C3H-2K cells) consisting essentially of polysaccharide RBS substance and pharmaceutically acceptable acid-addition salts thereof in a pharmaceutically effective amount, and a pharmaceutically acceptable vehicle, said polysaccharide RBS substance (1) having the following properties: it does not pass through a semipermeable membrane;
Claim 5
Dependent Claim
136 words
it is insoluble in alcohol, acetone, hexane, benzene, ethyl acetate, dimethylsulfoxide, ligroin, carbon tetrachloride, chloroform and ether, and is soluble or highly soluble in water; a 1% aqueous solution or suspension is neutral or slightly acidic; it reacts positively in the following reactions: the Molisch reaction, the anthrone-sulfuric acid reaction, the tryptophane-sulfuric acid reaction, the crystein-sulfuric acid reaction, the chromotrope-sulfuric acid reaction, the phenol-sulfuric acid reaction, the carbazole-sulfuric acid reaction, the biuret reaction, the ninhydrin reaction and the Lowry-Folin reaction; and negatively in the Elson-Morgan reaction and the starch-iodine reaction; it has the ultraviolet absorption spectrum depicted in FIG. 1 and the infrared absorption spectrum depicted in FIG. 2; and (2) containing glucose units as the sugar component, said glucose units being bonded by α-1,6-glucoside bonds as the main chain and said main chain is branched.
Claim 6
Dependent Claim
136 words
it is insoluble in alcohol, acetone, hexane, benzene, ethyl acetate, dimethylsulfoxide, ligroin, carbon tetrachloride, chloroform and ether, and is soluble or highly soluble in water; a 1% aqueous solution or suspension is neutral or slightly acidic; it reacts positively in the following reactions: the Molisch reaction, the anthrone-sulfuric acid reaction, the tryptophane-sulfuric acid reaction, the crystein-sulfuric acid reaction, the chromotrope-sulfuric acid reaction, the phenol-sulfuric acid reaction, the carbazole-sulfuric acid reaction, the biuret reaction, the ninhydrin reaction and the Lowry-Folin reaction; and negatively in the Elson-Morgan reaction and the starch-iodine reaction; it has the ultraviolet absorption spectrum depicted in FIG. 1 and the infrared absorption spectrum depicted in FIG. 2; and (2) containing glucose units as the sugar component, said glucose units being bonded by α-1,6-glucoside bonds as the main chain and said main chain is branched.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-11-01 | 1986-05-03 | 1986-11-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-11-01 | 1990-05-03 | 1990-11-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-11-01 | 1994-05-03 | 1994-11-02 | $7700.00 | Window Closed / Lapsed |