Process of microbial extraction of hydrocarbons from oil sands

Claim 2

A process for the separation of hydrocarbon residues from oil and tar sands which comprises contacting the sands under aerobic conditions with an aqueous medium containing hydrocarbon-metabolizing microorganisms capable of producing oxidase enzymes or oxygenase enzymes or both, in the presence of an added surfactant in an amount sufficient to provide surface tension of at most about 40 dynes/cm and in the presence of oxygen in an amount sufficient to ensure activity of said enzymes, whereby hydrocarbon residues are released from the sands.

Claim 3

The process according to claim 1, wherein terminal carbons of alkane molecules in the hydrocarbon residues are oxidized or aromatic compounds in the hydrocarbon residues are hydroxylated or both, whereby the hydrocarbon residues are released from the sands.

Claim 4

The process according to claim 1 or 2, wherein the sands are maintained in suspension in the medium by turbulence.

Claim 5

The process according to claim 1, when carried out in a reaction vessel.

Claim 6

The process according to claim 1, wherein molecular oxygen or air is introduced during the process.

Claim 7

A process for the extraction of hydrocarbon residues from oil and tar sands which comprises: culturing hydrocarbon-metabolizing microorganisms capable of producing oxidase enzymes or oxygenase enzymes or both, in an aqueous medium, contacting oil or tar sands with the microorganism-containing medium in the presence of an added surfactant in an amount sufficient to provide a surface tension of at most about 40 dynes/cm, agitating the mixture of sands and microorganism-containing medium under aerobic conditions to provide oxygen in an amount sufficient to ensure activity of said enzymes, whereby the hydrocarbon residues are released from the sands, and recovering the hydrocarbon residues.

Claim 8

A process for the extraction of hydrocarbon residues from oil and tar sands which comprises: culturing hydrocarbon-metabolizing microorganisms capable of producing oxidase enzymes or oxygenase enzymes or both, in an aqueous medium, contacting oil or tar sands with the microorganism-containing medium in the presence of an added surfactant in an amount sufficient to provide a surface tension of at most about 40 dynes/cm, agitating the mixture of sands and microorganism-containing medium under aerobic conditions to provide oxygen in an amount sufficient to ensure activity of said enzymes, whereby the hydrocarbon residues are released from the sands, and recovering the hydrocarbon residues.

Claim 9

The process according to claim 1, 2 or 6, wherein the concentration of microorganisms in the medium is about 1×10 9 microorganism/ml.

Claim 10

The process according to claim 1, 2 or 6, wherein the surfactant is anionic or nonionic and is biodegradable.

Claim 11

The process according to claim 1, 2 or 6, wherein the concentration of surfactant with respect to medium is in the range of about 0.01 to 0.0001%.

Claim 12

The process according to claim 1, 2 or 6, wherein the microorganisms comprise a mixed culture selected from the group consisting of microorganisms of the species Pseudomonas, Corynebacterium, Flavobacterium, Nocardia, Arthrobacter, Micrococcus, Mycobacterium, Streptomyces, Achromobacter and Bacillus.

Claim 13

The process according to claim 1, 2 or 6, wherein the microorganisms comprise a mixed culture of Bacillus subtilis, Pseudomonas fluorescens, Pseudomonas sp., Nocardia corallina, Corynebacterium sp. and Micrococcus sp.

Claim 14

The process according to claim 1, 2 or 6, wherein the microorganisms comprise a mixed culture of Arthrobacter sp., Corynebacterium sp. and Pseudomonas sp.

Claim 15

The process according to claim 1, 2 or 6, wherein the microorganisms comprise a mixed culture of Pseudomonas sp., Micrococcus sp. and Nocardia sp.

Claim 16

The process according to claim 1, 2 or 6, wherein the microorganisms comprise a mixed culture of Nocardia sp., Arthrobacter sp., Micrococcus sp., Corynebacterim sp., Pseudomonas sp. and Bacillis subtilis.

Claim 17

The process according to claim 1, 2 or 6, wherein the microorganisms comprise a pure culture selected from the group consisting of microorganisms of the species Pseudomonas, Corynebacterium, Flavobacterium, Nocardia, Arthrobacter, Micrococcus, Mycobacterium, Streptomcyes, Achrombacter, and Bacillus.

Claim 18

The process according to claim 1, 2 or 6, wherein the microorganisms comprise a pure culture selected from the group consisting of Pseudomonas arvilla, Pseudomonas dacunhae, Pseudomonas desmolytica, Pseudomonas sp. Arthrobacter oxydans, Arthrobacter simplex, Arthrobacter sp., Corynebacterium hydroxycarbooxydans, Corynebacterium dioxydans, Corynebacterium sp., Flavobacterium oxydans, Flavobacterium devorans, Flavobacterium sp., Nocardia corallina, Nocardia rubra, Nocardia opaca, Nocardia salmonicolor, Nocardia hydrocarbonoxydans, Nocardia sp., Micrococcus paraffinolyticus, Micrococcus sp., Mycobacterim rhodochrous, Mycobacterium sp., Achromobacter cycloclastes, Achromobacter pestifer and Achromobacter sp.