Patent US4347318A successfully refreshed from Google Patents.
US4347318A
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Method for producing L-threonine by fermentation
Assignee: Ajinomoto Co Inc
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Filing: 1980-04-02
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Granted: 1982-08-31
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Expires: 2000-04-02
Abstract
Abstract L-threonine is produced by incorporating into a recipient microorganism of the genus Escherichia which does not require L-threonine for growth, a plasmid, in which a deoxyribonucleic acid fragment which possesses genetic information relating to L-threonine synthesis obtained from a mutant resistant to alpha -amino- beta -hydroxy valeric acid of the genus Escherichia, has been inserted.
Inventors
- 👤 Kiyoshi Miwa
- 👤 Takayasu Tsuchida
- 👤 Osamu Kurahashi
- 👤 Shigeru Nakamori
- 👤 Konosuke Sano
- 👤 Haruo Momose
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 19:26
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/52; C12N1/00; C12N1/20; C12N1/205; C12P; C12P13/00; C12P13/04; C12P13/08; C12R; C12R2001/00; C12R2001/01; C12R2001/185; C12R2001/19; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/848; Y10S435/849
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
72 words
A method for producing L-threonine by fermentation which comprises culturing in a culture medium an L-threonine producing microorganism wherein said microorganism is produced by incorporating into a recipient microorganism of the genus Escherichia which does not require L-threonine for growth, a plasmid, in which a deoxyribonucleic acid fragment which possesses genetic information relating to L-threonine synthesis obtained from a mutant resistant to α-amino-β-hydroxy valeric acid of the genus Escherichia, has been inserted.
Claim 2
Independent Claim
72 words
A method for producing L-threonine by fermentation which comprises culturing in a culture medium an L-threonine producing microorganism wherein said microorganism is produced by incorporating into a recipient microorganism of the genus Escherichia which does not require L-threonine for growth, a plasmid, in which a deoxyribonucleic acid fragment which possesses genetic information relating to L-threonine synthesis obtained from a mutant resistant to α-amino-β-hydroxy valeric acid of the genus Escherichia, has been inserted.
Claim 3
Dependent Claim
15 words
The method of claim 1 wherein said L-threonine producing microorganism is Escherichia coli NRRL B-12100.
Claim 4
Dependent Claim
12 words
The method of claim 1, wherein the culture medium contains L-aspartic acid.
Claim 5
Dependent Claim
12 words
A bacterium of the genus Escherichia which is Escherichia coli NRRL B-12100.
Claim 6
Dependent Claim
12 words
A bacterium of the genus Escherichia which is Escherichia coli NRRL B-12100.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-08-30 | 1986-03-01 | 1986-08-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-08-30 | 1990-03-01 | 1990-08-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-08-30 | 1994-03-01 | 1994-08-31 | $7700.00 | Window Closed / Lapsed |