Patent US4346169A successfully refreshed from Google Patents.
US4346169A
Expired / Lapsed
US
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Method for production of L-arginine by fermentation
Assignee: Ajinomoto Co Inc
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Filing: 1980-09-15
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Granted: 1982-08-24
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Expires: 2000-09-15
Abstract
Abstract Mutants of the genus Bervibacterium or Corynebacterium are given resistance to keto-malonic acid, fluoro-malonic acid, monofluoro-acetic acid or aspartate antagonist and used to produce L-arginine by aerobic fermentation.
Inventors
- 👤 Kunihiko Akashi
- 👤 Yayoi Nakamura
- 👤 Takayasu Tsuchida
- 👤 Hiroe Yoshii
- 👤 Shigeho Ikeda
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 09:46
CPC Classifications
C; C12; C12P; C12P13/00; C12P13/04; C12P13/10; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/84; Y10S435/843
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
125 words
A method for the production of L-arginine by fermentation which comprises: (1) culturing aerobically in a culture medium a mutant capable of producing L-arginine of the genus Brevibacterium or Corynebacterium, and (2) recovering the L-arginine accumulated in the culture medium; said mutant being resistant to Keto-malonic acid, fluoro-malonic acid, monofluoro-acetic acid or aspartate-antagonist, wherein said mutant belongs to the species Brevibacterium flavum or Corynebacterium acetoacidophilum selected from the class consisting of ______________________________________
Brevibacterium flavum
NRRL B-12235
Brevibacterium flavum
NRRL B-12236
Brevibacterium flavum
NRRL B-12237
Brevibacterium flavum
NRRL B-12240
Brevibacterium flavum
NRRL B-12242
Brevibacterium flavum
NRRL B-12243
Brevibacterium flavum
NRRL B-12244
Brevibacterium flavum
NRRL B-12241
Brevibacterium flavum
NRRL B-12247
Corynebacterium acetoacidophilum
NRRL B-12238
Corynebacterium acetoacidophilum
NRRL B-12239
Corynebacterium acetoacidophilum
NRRL B-12245
Corynebacterium acetoacidophilum
NRRL B-12246
______________________________________
Claim 2
Independent Claim
125 words
A method for the production of L-arginine by fermentation which comprises: (1) culturing aerobically in a culture medium a mutant capable of producing L-arginine of the genus Brevibacterium or Corynebacterium, and (2) recovering the L-arginine accumulated in the culture medium; said mutant being resistant to Keto-malonic acid, fluoro-malonic acid, monofluoro-acetic acid or aspartate-antagonist, wherein said mutant belongs to the species Brevibacterium flavum or Corynebacterium acetoacidophilum selected from the class consisting of ______________________________________
Brevibacterium flavum
NRRL B-12235
Brevibacterium flavum
NRRL B-12236
Brevibacterium flavum
NRRL B-12237
Brevibacterium flavum
NRRL B-12240
Brevibacterium flavum
NRRL B-12242
Brevibacterium flavum
NRRL B-12243
Brevibacterium flavum
NRRL B-12244
Brevibacterium flavum
NRRL B-12241
Brevibacterium flavum
NRRL B-12247
Corynebacterium acetoacidophilum
NRRL B-12238
Corynebacterium acetoacidophilum
NRRL B-12239
Corynebacterium acetoacidophilum
NRRL B-12245
Corynebacterium acetoacidophilum
NRRL B-12246
______________________________________
Claim 3
Dependent Claim
23 words
The method according to claim 1 where the mutant resistant to keto-malonic acid is Brevibacterium flavum NRRL B-12242 or Brevibacterium flavum NRRL B-12245.
Claim 4
Dependent Claim
18 words
The method according to claim 1 where the mutant resistant to fluoro-malonic acid is Brevibacterium flavum NRRL B-12243.
Claim 5
Dependent Claim
28 words
The method according to claim 1 where the mutant resistant to monofluoro-acetic acid is Brevibacterium flavum NRRL B-12244 or Brevibacterium flavum NRRL B-12247, or Corynebacterium acetoacidophilum NRRL B-12246.
Claim 6
Dependent Claim
27 words
The method according to claim 1 where the mutant resistant to aspartate-antagonist is Brevibacterium flavum NRRL B-12235 or Corynebacterium acetoacidophilum NRRL B-12238 or Brevibacterium flavum NRRL B-12240.
References Cited (Backward Citations)
JPS5238088A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-08-23 | 1986-02-22 | 1986-08-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-08-23 | 1990-02-22 | 1990-08-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-08-23 | 1994-02-22 | 1994-08-24 | $7700.00 | Window Closed / Lapsed |