Imidazo-rifamycin derivatives with antibacterial utility

Claim 2

A compound of formula ##STR7## wherein: A is ##STR8## --x-- is a covalent chemical bond or nil; R is hydrogen or acetyl; R 1 and R 2 independently represent hydrogen, (C 1-4 ) alkyl, benzyloxy, mono- and di-(C 1-3 ) alkylamino-(C 1-4 ) alkyl, (C 1-3 )alkoxy- (C 1-4 )alkyl, hydroxymethyl, hydroxy-(C 2-4 )-alkyl, nitro or R 1 and R 2 taken together with two consecutive carbon atoms of the pyridine nucleus form a benzene ring unsubstituted or substituted by one or two methyl or ethyl groups; R 3 is a hydrogen atom or nil; with the proviso that, when A is A 1 , --x-- is nil and R 3 is a hydrogen atom; with the further proviso that, when A is A 2 , --x-- is a covalent chemical bond and R 3 is nil.

Claim 3

A compound as defined in claim 1, wherein A is A 1 or A 2 as above indicated, --x-- is a covalent chemical bond or nil, R is hydrogen or acetyl, R 1 and R 2 independently represent hydrogen, (C 1-4 )alkyl, benzyloxy, hydroxy-(C 2-4 ) alkyl, di-(C 1-3 ) alkylamino-(C 1-4 ) alkyl, nitro or R 1 and R 2 taken together with two consecutive carbon atoms of the pyridine nucleus form a benzene ring and R 3 is a hydrogen atom or nil; with the proviso that, when A is A 1 , --x-- is nil and R 3 is a hydrogen atom; with the further proviso that, when A is A 2 , --x-- is a covalent chemical bond and R 3 is nil.

Claim 4

A compound as defined in claim 1, wherein A is A 1 or A 2 as above indicated, --x-- is a covalent chemical bond or nil, R is acetyl, R 1 and R 2 independently represent hydrogen, (C 1-4 ) alkyl or R 1 and R 2 taken together with two consecutive carbon atoms of the pyridine nucleus form a benzene ring and R 3 is a hydrogen atom or nil; with the proviso that, when A is A 1 , --x-- is nil and R 3 is a hydrogen atom; with the further proviso that, when A is A 2 , --x-- is a covalent chemical bond and R 3 is nil.

Claim 5

A compound as defined in claim 1, which is 4-deoxy-4'-methyl-pyrido[1',2'-1,2]imidazo [5,4-c]rifamycin SV.

Claim 6

A compound as defined in claim 1, which is 4-deoxy-pyrido [1',2':1,2]imidazo [5,4-c] rifamycin SV.

Claim 7

A process for preparing a compound of formula ##STR9## wherein: A is ##STR10## --x-- is a covalent chemical bond or nil; R is hydrogen or acetyl; R 1 and R 2 independently represent hydrogen, (C 1-4 ) alkyl, benzyloxy, mono- and di-(C 1-3 )alkylamino(C 1-4 )alkyl, (C 1-3 )alkoxy- (C 1-4 )alkyl, hydroxymethyl, hydroxy-(C 2-4 )-alkyl, nitro or R 1 and R 2 taken together with two consecutive carbon atoms of the pyridine nucleus form a benzene ring unsubstituted or substituted by one or two methyl or ethyl groups; R 3 is a hydrogen atom or nil; with the proviso that, when A is A 1 , --x-- is nil and R 3 is a hydrogen atom; with the further proviso that, when A is A 2 , --x-- is a covalent chemical bond and R 3 is nil, which consists of reacting a molar amount of a 3-halorifamycin S of formula: ##STR11## wherein hal stands for a halogen atom and R is hydrogen or acetyl, with a molar excess of a compound of formula ##STR12## where R 1 and R 2 are as above defined, in the presence of an inert organic solvent, at room temperature, for a period of time comprised between about 1 and about 4 hours, whereby a compound of formula 1 is obtained in which A is A 2 , R, R 1 , and R 2 are as above defined, --x-- is a covalent chemical bond and R 3 is nil, which can be transformed into the corresponding compounds of formula 1 wherein A is A 1 , R, R 1 and R 2 are as above defined, --x-- is nil and R 3 stands for a hydrogen atom, by treatment with a suitable reducing agent, in an inert organic solvent; said process being further characterized in that: (a) the compound of formula 1 wherein A identifies the structure A 1 , R, R 1 and R 2 are as above defined, --x-- is nil and R 3 stands for a hydrogen atom, is transformed into the corresponding compounds of formula 1 wherein A is A 2 , R, R 1 and R 2 are as above defined, --x-- is a covalent chemical bond and R 3 represents nil, by treatment with a suitable oxidizing agent selected from manganese dioxide, lead tetracetate, dichloro-dicuano-benzoquinone, 2,3,4,5-tetrachloro-benzoquinone, 2,3,5,6-tetrachloro-benzoquinone and analogs; (b) the compound of formula 1 wherein R is acetyl and is converted into the corresponding compound of formula 1 wherein R is hydrogen, by mild alkaline hydrolysis.

Claim 8

A process for preparing a compound of formula ##STR9## wherein: A is ##STR10## --x-- is a covalent chemical bond or nil; R is hydrogen or acetyl; R 1 and R 2 independently represent hydrogen, (C 1-4 ) alkyl, benzyloxy, mono- and di-(C 1-3 )alkylamino(C 1-4 )alkyl, (C 1-3 )alkoxy- (C 1-4 )alkyl, hydroxymethyl, hydroxy-(C 2-4 )-alkyl, nitro or R 1 and R 2 taken together with two consecutive carbon atoms of the pyridine nucleus form a benzene ring unsubstituted or substituted by one or two methyl or ethyl groups; R 3 is a hydrogen atom or nil; with the proviso that, when A is A 1 , --x-- is nil and R 3 is a hydrogen atom; with the further proviso that, when A is A 2 , --x-- is a covalent chemical bond and R 3 is nil, which consists of reacting a molar amount of a 3-halorifamycin S of formula: ##STR11## wherein hal stands for a halogen atom and R is hydrogen or acetyl, with a molar excess of a compound of formula ##STR12## where R 1 and R 2 are as above defined, in the presence of an inert organic solvent, at room temperature, for a period of time comprised between about 1 and about 4 hours, whereby a compound of formula 1 is obtained in which A is A 2 , R, R 1 , and R 2 are as above defined, --x-- is a covalent chemical bond and R 3 is nil, which can be transformed into the corresponding compounds of formula 1 wherein A is A 1 , R, R 1 and R 2 are as above defined, --x-- is nil and R 3 stands for a hydrogen atom, by treatment with a suitable reducing agent, in an inert organic solvent; said process being further characterized in that: (a) the compound of formula 1 wherein A identifies the structure A 1 , R, R 1 and R 2 are as above defined, --x-- is nil and R 3 stands for a hydrogen atom, is transformed into the corresponding compounds of formula 1 wherein A is A 2 , R, R 1 and R 2 are as above defined, --x-- is a covalent chemical bond and R 3 represents nil, by treatment with a suitable oxidizing agent selected from manganese dioxide, lead tetracetate, dichloro-dicuano-benzoquinone, 2,3,4,5-tetrachloro-benzoquinone, 2,3,5,6-tetrachloro-benzoquinone and analogs; (b) the compound of formula 1 wherein R is acetyl and is converted into the corresponding compound of formula 1 wherein R is hydrogen, by mild alkaline hydrolysis.

Claim 9

A process as defined in claim 6, wherein from about 1 to about 8 molar equivalents of the compound of formula III are used for each molar equivalent of the rifamycin derivative of formula II.

Claim 10

A process as defined in claim 6, wherein hal represents bromine or iodine.

Claim 11

A process as defined in claim 6, wherein the organic solvent is selected from the group consisting of aliphatic alkanols from 1 to 4 carbon atoms and halogenated hydrocarbons from 1 to 2 carbon atoms.

Claim 12

A pharmaceutical antibacterial composition containing, as the active ingredient, an antibacterial effective amount of a compound as defined in claim 1 in admixture with one or more solid or liquid pharmaceutically acceptable carriers.

Claim 13

A pharmaceutical antibacterial composition as defined in claim 10, wherein the active ingredient is 4-deoxy-4'-methyl-pyrido[1',2':1,2]imidazo[4,5-c]rifamycin SV.

Claim 14

A pharmaceutical antibacterial composition as defined in claim 10, wherein the active ingredient is 4-deoxy-pyrido[1',2':1,2]imidazo[5,4-c]rifamycin SV.

Claim 15

The method of treating antibacterial diseases, which comprises administering to mammals suffering from said disease an antibacterial effective amount of a compound as defined in claim 1.