US4340673A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process of producing modified glucans as anti-caries agent
Assignee: Merck and Co Inc
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Filing: 1980-11-07
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Granted: 1982-07-20
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Expires: 2000-11-07
Abstract
Abstract Certain glucans modify the biosynthetic route of extracellular polysaccharides involved in dental plaque development causing critical loss of adhesiveness. They interfere with the biosynthesis of cell-bound polysaccharides reducing and preventing agglutination of cells. They thus aid in the prevention of dental plaque formation or concomitant dental caries and periodontal disease.
Inventors
- 👤 Thomas H. Stoudt
- 👤 Karl H. Nollstadt
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 15:16
CPC Classifications
A; A61; A61K; A61K31/00; A61K31/70; A61K31/715; A61K8/00; A61K8/18; A61K8/72; A61K8/73; A61Q; A61Q11/00; C; C12; C12P; C12P19/00; C12P19/18; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/885
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
78 words
A process for the preparation of a glucan with a molecular weight in excess of 500,000, consisting predominantly of α-(1→3)glucosidic bonds and inert to enzymatic attack by an endo-α-(1→3)glucan-3-glucanohydrolase which comprises cell-free biosynthesis of plaque polysaccharides in a biosynthetic medium containing glucosyl transferase derived from Streptococcus mutans, sucrose, and from 5-40 units/ml of the endo-α-(1→3)glucan-3-glucanohydrolase for 24-48 hours at 37° C., inactivating the enzymes, removing insoluble polysaccharides and adding the miscible organic solvent to precipitate the desired glucan.
Claim 2
Dependent Claim
78 words
A process for the preparation of a glucan with a molecular weight in excess of 500,000, consisting predominantly of α-(1→3)glucosidic bonds and inert to enzymatic attack by an endo-α-(1→3)glucan-3-glucanohydrolase which comprises cell-free biosynthesis of plaque polysaccharides in a biosynthetic medium containing glucosyl transferase derived from Streptococcus mutans, sucrose, and from 5-40 units/ml of the endo-α-(1→3)glucan-3-glucanohydrolase for 24-48 hours at 37° C., inactivating the enzymes, removing insoluble polysaccharides and adding the miscible organic solvent to precipitate the desired glucan.
Claim 3
Dependent Claim
78 words
A process for the preparation of a glucan with a molecular weight in excess of 500,000, consisting predominantly of α-(1→6)glucosidic bonds and inert to enzymatic attack by an endo-α-(1→6)glucan-6-glucanohydrolase which comprises cell-free biosynthesis of plaque polysaccharides in a biosynthetic medium containing glucosyl transferase derived from Streptococcus mutans, sucrose, and from 5-40 units/ml of the endo-α-(1→6)glucan-6-glucanohydrolase for 24-48 hours at 37° C., inactivating the enzymes, removing insoluble polysaccharides and adding a miscible organic solvent to precipitate the desired glucan.
Claim 4
Dependent Claim
78 words
A process for the preparation of a glucan with a molecular weight in excess of 500,000, consisting predominantly of α-(1→6)glucosidic bonds and inert to enzymatic attack by an endo-α-(1→6)glucan-6-glucanohydrolase which comprises cell-free biosynthesis of plaque polysaccharides in a biosynthetic medium containing glucosyl transferase derived from Streptococcus mutans, sucrose, and from 5-40 units/ml of the endo-α-(1→6)glucan-6-glucanohydrolase for 24-48 hours at 37° C., inactivating the enzymes, removing insoluble polysaccharides and adding a miscible organic solvent to precipitate the desired glucan.
Claim 5
Dependent Claim
10 words
The process of claim 1 wherein the endo-α-(1→3)glucan-3-glucanohydrolase is cariogenanase.
Claim 6
Dependent Claim
10 words
The process of claim 1 wherein the endo-α-(1→6)glucan-6-glucanohydrolase is dextranase.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-07-19 | 1986-01-18 | 1986-07-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-07-19 | 1990-01-18 | 1990-07-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-07-19 | 1994-01-18 | 1994-07-20 | $7700.00 | Window Closed / Lapsed |